159,258 results

1. pLX317-QKI

   Plasmid

   #115441

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   QKI (QKI Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p-dCas9-JMJD2A-Hygro

   Plasmid

   #104410

   Purpose

   transient expression of dCas9-JMJD2A fusion protein

   Depositor

   Stephan Beck

   Insert

   JMJD2A (KDM4A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPGK-Cre-bpA

   Plasmid

   #11543

   Purpose

   Expresses Cre recombinase from mouse PGK promoter in mammalian cells.

   Depositor

   Klaus Rajewsky

   Insert

   Phosphoglycerate kinase 1 promoter
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse PGK

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas3cRh

   Plasmid

   #133773

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Joseph Bondy-Denomy

   Article

   Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   Cas7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF-GFP

   Plasmid

   #11154

   Purpose

   Mammalian expression vector for expression of GFP (EF1a promoter)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   EF1 alpha promoter (EEF1A1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLifeAct_mScarlet_N1

   Plasmid

   #85054

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   LifeAct
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Hs.cargo(Cas9)

   Plasmid

   #174864

   Purpose

   Expresses human Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by HsPeg10 UTRs
   

   Use

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-GRP78/BiP

   Plasmid

   #32701

   Depositor

   Richard C. Austin

   Article

   Werstuck et al J Clin Invest. 2001 May;107(10):1263-73.

   Insert

   GRP78/BiP (HSPA5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]

    Plasmid

    #84446

    Purpose

    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Has Service

    AAV1, AAV5, and AAV8

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CAG

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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