We narrowed to 18,591 results for: IRE

1. pMCSG7-10xHis-ecRNHI*

   Plasmid

   #219523

   Purpose

   bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

   Depositor

   Arthur Palmer

   Article

   Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

   Insert

   cysteine-free variant of the E. coli ribonuclease HI gene
   

   Use

   Tags

   N terminal 10xHis Tag

   Expression

   Bacterial

   Mutation

   Cysteine free variant

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cut190*_pET21b

   Plasmid

   #215404

   Purpose

   Expression construct for Cut190* gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   Cut190*
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S226P/R228S

   Promoter

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mFzd4CRDL(42-203)-6xHis_pAcGP67a

   Plasmid

   #216379

   Purpose

   Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Frizzled-4 (Fzd4 Mouse)
   

   Use

   Baculovirus

   Tags

   6xHis and GP64 signal sequence

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBa.Halo-KIF1A tail

   Plasmid

   #218800

   Purpose

   Mammalian expression of rat Kif 1A

   Depositor

   Marvin Bentley

   Article

   Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

   Insert

   Kif 1A (Kif1a Rat)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta actin

   Available since

   May 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. RoL10X_pET29b

   Plasmid

   #215427

   Purpose

   Expression construct for RoL10X gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   RoL10X
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. NylB-SCY_pET29b

   Plasmid

   #215415

   Purpose

   Expression construct for NylB-SCY gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB-SCY
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R187S/F264C/D370Y

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBDP

   Plasmid

   #217827

   Purpose

   Stuffer plasmid with golden gate compatible bidirectional promoter

   Depositor

   Timothy Whitehead

   Article

   Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

   Insert

   Gal1/Gal10 Bidirectional Promoter
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-h.Rhno1 Plk1mut7A-C-PAMmut-Myc-DDK-P2A-Puro

   Plasmid

   #211616

   Purpose

   Rhno1 Plk1mut7A

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   hRHNO1 (RHNO1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S51A, T52A, S141A, S162A, S163A, S164A, S178A

   Promoter

   CMV

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMBA337

   Plasmid

   #214750

   Purpose

   Medium copy thyA-infA plasmid containing bicistronic gfp-specR for use in mouse work validation

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23119-RiboJ-RBS(21992)-gfpmut3-RBS(4533)-specR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-PTK6-YF

    Plasmid

    #210715

    Purpose

    Construct for stable expression of constitutively active human PTK6 in mammalian cells.

    Depositor

    Angela Tyner

    Article

    Zheng et al Mol Cell Biol. 2010 Sep;30(17):4280-92.

    Insert

    Protein tyrosine kinase 6 (PTK6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Tyrosine 447 changed to Phenylalanine

    Promoter

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. hLig4 guide2 in pX458

    Plasmid

    #211536

    Purpose

    sgRNA-2 against Lig4

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    sgRNA hLig4 (LIG4 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. hLig4 guide1 in pX458

    Plasmid

    #211535

    Purpose

    sgRNA-1 against Lig4

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    sgRNA hLig4 (LIG4 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-h.Rhno1 delta TOPBP1 (T202A)-C-Myc-P2A-Puro

    Plasmid

    #211619

    Purpose

    Rhno1 delta TOPBP1 (T202A)

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    hRHNO1 (RHNO1 Human)
    

    Use

    Tags

    myc-flag

    Expression

    Mammalian

    Mutation

    T202A

    Promoter

    CMV

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TLNRD1_2T_eGFP

    Plasmid

    #208883

    Purpose

    Express eGFP-TLNRD1 with the L191T and A225T mutations in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    TLNRD1 (TLNRD1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutations L191T and A225T

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TLNRD1_2E_eGFP

    Plasmid

    #208884

    Purpose

    Express eGFP-TLNRD1 with the K192E and R233E mutations in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    TLNRD1 (TLNRD1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutations K192E and R233E

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmcb306-GFP-Rab12-mito

    Plasmid

    #207349

    Purpose

    Mammalian expression of Rab12-mito

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    Rab12-mito (RAB12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMX_mePOU

    Plasmid

    #206396

    Purpose

    Expresses mouse ePOU in mammalian cells, for retrovirus generation.

    Depositor

    Ralf Jauch

    Article

    Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

    Insert

    ePOU
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJAT58

    Plasmid

    #204304

    Purpose

    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJAT71

    Plasmid

    #204305

    Purpose

    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR- flag-Lag43-iCAM-tether

    Plasmid

    #205211

    Purpose

    This vector encodes of the synCAM tether (iCAM1) and GFP nanobody LAG43

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 tether and Lag43 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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