We narrowed to 19,203 results for: IRE

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EF1a_SPIC_P2A_Hygro_Barcode

Plasmid

#120484

Purpose

Barcoded lentiviral vector to express SPIC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPIC (SPIC Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK1(1-828)

Plasmid

#38198

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 829-1051

Available Since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
pL106P-LUC-ENTR1A(w451-1)

Plasmid

#17455

Purpose

Gateway entry vector, Degron N-term Luciferase

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

FKBP-Luciferase fusion (MTOR firefly)

Use

Luciferase; Entry vector

Tags

FKBP12DD

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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FN207

Plasmid

#50497

Depositor

Kenneth Yamada

Article

Danen et al J Biol Chem. 1995 Sep 15;270(37):21612-8

Insert

NM_001306129.1 (FN1 Human)

Expression

Bacterial

Mutation

repeats 6-10

Promoter

T7

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pRRG199-bmp4

Plasmid

#99076

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Bmp4 (also known as bmp)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Bmp4 (also known as bmp) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEE495

Plasmid

#149291

Purpose

T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAsCpf1GG

Plasmid

#131011

Purpose

Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of AsCas12a
GFP expression cassette

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop

Plasmid

#169369

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop

Use

CRISPR

Expression

Insect

Promoter

CPIJ039653

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-SpCas9_sgRNA-site2 (RTW448)

Plasmid

#160137

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-24

Plasmid

#172750

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-24

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-9

Plasmid

#172742

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-9

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSuper mouse DGCR8-3

Plasmid

#14774

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (Dgcr8 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pLenti CRISPR V2 sgMTFMT_1

Plasmid

#106318

Purpose

Express Cas9 and sgRNA targeting MTFMT

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTFMT

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-AD1-deletion

Plasmid

#75265

Purpose

Expresses a deletion mutant of human TTF-1 lacking the activation domain 1

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

TTF-1 (NKX2-1 Human)

Tags

none

Expression

Mammalian

Mutation

AD1 (activation domain 1) deleted

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-AD2-deletion

Plasmid

#75266

Purpose

Expresses a deletion mutant of human TTF-1 lacking the activation domain 2

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

TTF-1 (NKX2-1 Human)

Tags

none

Expression

Mammalian

Mutation

AD2 (activation domain 2) deleted

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJAF216

Plasmid

#52405

Purpose

Expresses Flag-tagged mouse Arf4

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf4 (Arf4 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pLENTI CMV GFP-TREX1 V235fs BLAST

Plasmid

#164236

Purpose

lentiviral plasmid for GFP-TREX1 V235fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

V235 frame shift mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shCUX1-5328

Plasmid

#100815

Purpose

Lentiviral vector expressing a doxycycline-inducible short hairpin RNA against CUX1 sequence starting at 5328 of M74099

Depositor

Alain Nepveu

Article

Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

Insert

TGCTGTTGACAGTGAGCGTCTTCTCGTTTGAAACTTTGAATAGTGAAGCCA

Use

Lentiviral

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-RH-mycStrep

Plasmid

#31839

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

R155H

Promoter

CMV

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 3xFLAG TPP1 88-249

Plasmid

#53549

Purpose

mammalian expression of TPP1 OB-fold domain

Depositor

Kathleen Collins

Article

Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

Insert

TPP1 (ACD Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

aa 88-249

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only