We narrowed to 9,182 results for: Pol

1. pMXs-LN

   Plasmid

   #188231

   Purpose

   Polycistronic human LIN28 and NANOG were cloned into the pMXs vector

   Depositor

   Young Tang

   Article

   Wang et al Biol Open. 2019 Dec 5;8(12). pii: 8/12/b

   Insert

   LIN28A and NANOG separated by 2A sequence (LIN28A, NANOG Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

   Plasmid

   #169166

   Purpose

   Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

   Insert

   3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)
   

   Tags

   3xFlag-6His

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVD1 Multiplexing Edit E4 (GB2241)

   Plasmid

   #160563

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for position [4-5] of a polycistronic tRNA-gRNA.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   Multiplexing Edit (E4)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET45b-tsr(B/X) (6X-His-Dm-Cofilin)

   Plasmid

   #128278

   Purpose

   Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture

   Depositor

   Anna Sokac

   Article

   Figard et al Cell Rep. 2019 Mar 26;26(13):3493-3501.e

   Insert

   twinstar (tsr) (tsr Fly)
   

   Tags

   6X Histidine tag

   Expression

   Bacterial

   Mutation

   Met1 changed to Pro1; Cys77 silent basepair mutat…

   Promoter

   T7

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. vipr1_L (OZ559)

   Plasmid

   #28084

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting vipr1 (LOC100334247 Zebrafish)
   

   Use

   Zebrafish targeting

   Available Since

   March 14, 2011

   Availability

   Academic Institutions and Nonprofits only

6. vipr1_R (OZ560)

   Plasmid

   #28085

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting vipr1 (LOC100334247 Zebrafish)
   

   Use

   Zebrafish targeting

   Available Since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

7. CD44v-EC-His (pcDNA3)

   Plasmid

   #238418

   Purpose

   Expresses the polyhistidine-tagged extracellular region of CD44v (CD44v-EC-His)

   Depositor

   Amy Shiu Lee

   Article

   Tseng et al Life Sci Alliance. 2019 Aug 15;2(4):e201

   Insert

   CD44 containing extracellular region of CD44v (CD44 Human)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-LacI-Cyclin B1 ND

   Plasmid

   #234706

   Purpose

   allows tethering of non degradable (ND) cyclin B1 protein to lacO repeats in specific chromatin loci

   Depositor

   Lienhard Schmitz

   Article

   Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBacI-KDAC7 CD

   Plasmid

   #224345

   Purpose

   Expresses human KDAC7 (HDAC7) catalytic domain in insect cells

   Depositor

   Terry Watt

   Article

   Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

   Insert

   KDAC7 (HDAC7 Human)
   

   Tags

   TEV-cleavable His6

   Expression

   Insect

   Mutation

   Only includes residues 521-942 (catalytic domain).

   Promoter

   Polyhedrin

   Available Since

   Oct. 22, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pFB1.HMBP.PrS.CBX8ΔChromo

    Plasmid

    #224708

    Purpose

    Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 chromodomain truncation (CBX8 Human)
    

    Tags

    HMBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFBOH-MHL.mEGFP-CBX8

    Plasmid

    #224710

    Purpose

    Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 (CBX8 Human)
    

    Tags

    mEGFP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pFB1.HMBP.PrS.CBX8

    Plasmid

    #224707

    Purpose

    Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 (CBX8 Human)
    

    Tags

    HMBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXPR_003.gEZH2

    Plasmid

    #220318

    Purpose

    Expresses guide RNA for CRISPR/Cas9 knockout of EZH2 for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 gRNA (EZH2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

    Plasmid

    #216378

    Purpose

    Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

    Depositor

    William Weis

    Article

    Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    HA signal sequence-FLAG

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG416GAL10-POWV_NS2B-GS-NS3

    Plasmid

    #203471

    Purpose

    Expresses POWV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    POWV NS2B-GS-NS3 (POLY )
    

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG416GAL10-LGTV_NS2B-GS-NS3

    Plasmid

    #203468

    Purpose

    Expresses LGTV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    LGTV NS2B-GS-NS3 (POLY )
    

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG416GAL10-ZIKV_NS2B-GS-NS3

    Plasmid

    #203477

    Purpose

    Expresses ZIKV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    ZIKV NS2B-GS-NS3 (POLY )
    

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 2

    Plasmid

    #175816

    Purpose

    pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 2 (AT4G16143.1) without stop codon for C-terminal epitope tags

    Depositor

    Mark Banfield, Lennart Wirthmueller

    Article

    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

    Insert

    IMPORTIN ALPHA ISOFORM 2 (IMPA-2 Mustard Weed)
    

    Use

    Pentr plasmid for gateway cloning

    Tags

    none

    Mutation

    natural polymorphism P65S, annotated in Genbank f…

    Promoter

    none

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p35S_GFP-EVDinter-GUS

    Plasmid

    #167123

    Purpose

    Plant binary expression vector containing the intron and proximal polyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.

    Depositor

    Olivier Voinnet, Arturo Marí-Ordóñez

    Article

    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

    Insert

    EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)
    

    Tags

    GUS and mGFP5

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVD1 M3_3-pTRNA-scf 2.1 (GB2075)

    Plasmid

    #160560

    Purpose

    tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M3_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    M3_3-pTRNA-scf 2.1
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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