We narrowed to 63,772 results for: cell

1. pGL2-Mcl-1

   Plasmid

   #19132

   Depositor

   Wafik El-Deiry

   Article

   Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

   Insert

   Mcl-1 promoter (MCL1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. shRNA Caspase 1 (pSicoR mCherry)

   Plasmid

   #53575

   Purpose

   shRNA against human pro-caspase 1

   Depositor

   Warner Greene

   Article

   Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

   Insert

   shRNA Caspase 1 (CASP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 for shRNA; EF1a for mCherry

   Available Since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

   Plasmid

   #134925

   Purpose

   Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PMXS-GOT1

   Plasmid

   #72872

   Purpose

   The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   GOT1 (GOT1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   sgRNA resistant human GOT1, 7 silent mutations c1…

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

   Plasmid

   #134922

   Purpose

   Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHL-EF1a-SphcCas9-iP-A

   Plasmid

   #60599

   Purpose

   Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Insert

   CRISPR Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Codon usage optimized for human usage

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

   Plasmid

   #134924

   Purpose

   Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO/GFP-GIGYF1

   Plasmid

   #141188

   Purpose

   Expresses GFP-GIGYF1 in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

   Insert

   GIGYF1 (GIGYF1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

   Plasmid

   #118594

   Purpose

   Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   sgRNA/Puro-T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Gfp-zf-vasa

    Plasmid

    #167323

    Purpose

    Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

    Depositor

    Goro Yoshizaki

    Article

    Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

    Insert

    zf vasa 3'UTRs (ddx4 Zebrafish)
    

    Expression

    Bacterial

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. TAPBPL pmRFP-N2 (721)

    Plasmid

    #62000

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    TAPBPL (TAPBPL Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pDB-hPER2-Luciferase-hvTK-bla

    Plasmid

    #179450

    Purpose

    Donor vector to knock in firefly Luciferase C-terminal to human PER2 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    Luciferase
    

    Use

    CRISPR

    Tags

    His/Flag

    Promoter

    None

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDB-hPER2-mClover3-CD4-bla

    Plasmid

    #179448

    Purpose

    Donor vector to knock in mClover3 N-terminal to human PER2 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    mClover3
    

    Use

    CRISPR

    Tags

    His/Flag

    Promoter

    None

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid

    #134923

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57.1 _3xHA-Ago2

    Plasmid

    #73538

    Purpose

    Inducible lentiviral expression of Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    Ago2 (Ago2 Mouse)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. mRFP-FKBP12-5ptpase domain

    Plasmid

    #67516

    Purpose

    Recruitable human type IV 5-phosphatase domain (214-644)

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)
    

    Tags

    mRFP

    Mutation

    INPP5E has C641A to destroy the C-terminal CAAX d…

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pLX317 YAP1

    Plasmid

    #193683

    Purpose

    Constitutive lentiviral expression of YAP1

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. TetO-FUW-Ezh2

    Plasmid

    #118587

    Purpose

    Expresses Ezh2 from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Ezh2 (Ezh2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMIG-hNFATc1/bC

    Plasmid

    #74049

    Purpose

    retroviral expression plasmid for human NFATc1/bC

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc1, isoform beta-C (NFATC1 Human)
    

    Use

    Retroviral

    Tags

    no tag

    Expression

    Mammalian

    Promoter

    pMSCV-LTRs

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACT1:Cas9-GFP, U6:sgTK

    Plasmid

    #122852

    Purpose

    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Inserts

    Cas9-GFP
    U6-sgTK
    

    Use

    CRISPR; Cas9-gfp plasmid for genome editing of cr…

    Tags

    eGFP

    Promoter

    Cryptosporidium parvum U6 and Cryptosporidium par…

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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