We narrowed to 21,605 results for: ESC;

1. pGBW-m4046764

   Plasmid

   #145673

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pWZL-Stat5a1*6

   Plasmid

   #130669

   Purpose

   Retroviral vector for the expression of constitutive Stat5a (hygromycin resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

   Insert

   Stat5a (Stat5a Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Histidine 298 to Arginine and Serine 710 …

   Promoter

   LTR

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Lyn-GR-AKAR3

   Plasmid

   #173016

   Purpose

   Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Lyn-GR-AKAR3
   

   Use

   Tags

   EGFP, N-terminal targeting sequence from Lyn kina…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Clover-Lysosomes-20

   Plasmid

   #56528

   Purpose

   Localization: Lysosome Membrane, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   LAMP1
   

   Use

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pET/LEGH

   Plasmid

   #108226

   Purpose

   Bacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag

   Depositor

   Didier Picard

   Article

   Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

   Insert

   LBT-EGFP-His6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7, lacO

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. POM43-mCherry

   Plasmid

   #69857

   Purpose

   N-terminal tagging with mCherry using LEU2 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mCherry minus start and stop codon
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSA10(gfcB-His-CD)

   Plasmid

   #176194

   Purpose

   expresses 3 overlapping genes (gfcB-6xHis, gfcC, gfcD) from this one operon

   Depositor

   Mark Saper

   Article

   Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

   Insert

   gfcBCD
   

   Use

   Tags

   GfcB-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBH31(gfcD)

   Plasmid

   #176199

   Purpose

   Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm

   Depositor

   Mark Saper

   Article

   Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

   Insert

   gfcD
   

   Use

   Tags

   pelC leader-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBH31(gfcD-10xHis)

   Plasmid

   #176200

   Purpose

   Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm

   Depositor

   Mark Saper

   Article

   Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

   Insert

   gfcD
   

   Use

   Tags

   pelC leader-10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLENTI_RPS10_K138/139R

    Plasmid

    #127138

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS10 (RPS10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K138R/K139R

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM6-Pc4CL-CmCHS-MsCHI

    Plasmid

    #73398

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Pc4CL
    CmCHS
    MsCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTH-PanCen TALE-mVenusN

    Plasmid

    #191395

    Purpose

    TALE fused to N-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    N-terminal part of split mVenus
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCitrine-VE-Cadherin-N-10

    Plasmid

    #56319

    Purpose

    Localization: Tight Junctions, Excitation: 516, Emission: 529

    Depositor

    Michael Davidson

    Article

    Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

    Insert

    VE-Cadherin (CDH5 Human)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    I503T in VE-Cadherin

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pAL725

    Plasmid

    #119862

    Purpose

    precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Leucine 244 to Cysteine, Aspartate 287 to Cysteine

    Promoter

    T5

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEos2-H1-10

    Plasmid

    #57383

    Purpose

    Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    H1 (H1f0 Mouse)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

    Plasmid

    #45578

    Purpose

    Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    coY2 Ani
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGE12

    Plasmid

    #79469

    Purpose

    sgRNA shuttle vector; module 5

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGE13

    Plasmid

    #79471

    Purpose

    sgRNA shuttle vector; module 6

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGE14

    Plasmid

    #79473

    Purpose

    sgRNA shuttle vector; module 7

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDGE15

    Plasmid

    #79474

    Purpose

    sgRNA shuttle vector; module 8E

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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