19,824 results

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CMV::SypHy A4

Plasmid

#24478

Depositor

Leon Lagnado

Article

Granseth et al Neuron. 2006 Sep 21. 51(6):773-86.

Insert

SypHy (Syp Rat)

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

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CD47-bio-His

Plasmid

#53150

Purpose

Expresses full-length CD47 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

CD47 (CD47 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV Myc T58A puro

Plasmid

#18773

Depositor

Scott Lowe

Article

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

myc T58A (MYC Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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HA-GFP

Plasmid

#137763

Purpose

Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5RGDPK7

Plasmid

#198291

Purpose

Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions)

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5RGDPK7

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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plRL19

Plasmid

#163634

Purpose

L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Insert

L5 Integrase

Expression

Bacterial

Promoter

Mycobacterial Optimized Promoter (MOP) and Tet-On

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-shBRCA1 #1

Plasmid

#44594

Depositor

Eros Lazzerini Denchi

Article

Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

Insert

BRCA1 (Brca1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only
pBluescriptSKII+ U6-sgRNA(F+E) empty

Plasmid

#74707

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence

Expression

Mammalian

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-PH-PLC-delta-EGFP

Plasmid

#136998

Purpose

Lentiviral EGFP lipid sensor for PI(4,5)P2

Depositor

Ken-Ichi Takemaru

Insert

PH-PLC-delta-EGFP

Use

Lentiviral

Tags

EGFP

Promoter

EF1 alpha

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-SMYD2

Plasmid

#85658

Purpose

retroviral expression of human SMYD2

Depositor

Julien Sage

Article

Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

Insert

SMYD2 (SMYD2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

LTR

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Myc-YAP-S94A

Plasmid

#33094

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S94A

Promoter

CMV/MSV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pAM2991

Plasmid

#40248

Depositor

Susan Golden

Article

Ivleva et al EMBO J. 2005 Mar 23;24(6):1202-10. Epub

Insert

One Step Cloneing Vector for Overexpression

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pET His10 TEV LIC cloning vector (2B-T-10)

Plasmid

#78173

Depositor

Scott Gradia

Type

Empty backbone

Tags

10xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSAG1::CAS9-U6::sgUPRT

Plasmid

#54467

Purpose

Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.

Depositor

David Sibley

Article

Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

Insert

SAG1 5' UTR

Use

CRISPR; Toxoplasma expression

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp2-Flag

Plasmid

#11811

Depositor

Guy Salvesen

Insert

Caspase-2 (CASP2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

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HA-RNF4/pRK5

Plasmid

#59743

Purpose

Expresses RNF4 in mammalian cells

Depositor

Xiaolu Yang

Article

Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

Insert

RNF4 (RNF4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pS-cr:GFP

Plasmid

#196295

Purpose

35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs

Use

CRISPR

Tags

SpeI-DR-BsaI-DR-SpeI

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-Spike

Plasmid

#145031

Purpose

Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

Depositor

Fang Li

Article

Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

Insert

SARS-CoV Spike

Tags

C9 and CD5 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMU-NUCr

Plasmid

#61168

Purpose

Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

NLS-mCherry-GUS

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-NSP4SA11

Plasmid

#89171

Purpose

Express Rotavirus SA11 NSP4 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP4

Expression

Mammalian

Promoter

T7 promotor

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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