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pCMV-haA3A-CBE-VA-NG

Plasmid

#220896

Purpose

Express haA3A-CBE-VA-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-VA-NG

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LINXa3

Plasmid

#73614

Purpose

Light-induced nuclear export switch for microscopy in mammalian cells.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

LINXa3

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

Chicken B-Actin

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-BE-YE

Plasmid

#107686

Purpose

Expresses dLbCpf1-BE-YE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE-YE

Expression

Mammalian

Mutation

APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo

Plasmid

#78352

Purpose

Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-KRAB

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ub2-UBB+1

Plasmid

#23968

Depositor

Nico Dantuma

Article

Lindsten et al J Cell Biol. 2002 Apr 29. 157(3):417-27.

Insert

Ubiquitin B +1 transcript (UBB Human)

Expression

Mammalian

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_ORF3b_HA_BASU

Plasmid

#153992

Purpose

Express Orf3b gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf3b

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf45

Plasmid

#12733

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf45

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DUSP6

Plasmid

#40772

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

Insert

DUSP6 (DUSP6 Human)

Tags

6His and TEV

Expression

Bacterial

Mutation

Starting Met removed

Promoter

T7

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)

Plasmid

#139445

Purpose

Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag E2-crimson

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-GFPg1 (BB09)

Plasmid

#139461

Purpose

Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

GFP-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pX330.Pten.a

Plasmid

#66587

Purpose

sgRNA for Pten deletion

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

Pten deletion (Pten Mouse)

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-LGC-53

Plasmid

#86399

Purpose

Mammalian expression of codon-optimized dopamine receptor LGC-53

Depositor

Steven Mennerick

Article

Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802

Insert

LGC-53 (lgc-53 Nematode, Synthetic)

Expression

Mammalian

Mutation

Codon optimized for mammalian expression; Signal …

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA GFP10-RHOB

Plasmid

#182237

Purpose

Expresses RHOB GTPase with tripartite split GFP10 tag

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

ras homolog family member B (RHOB Human)

Tags

GFP10 (split-GFP tripartite)

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-evoA1-seBE4max-IRES

Plasmid

#174699

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

evoA1-seBE4max-IRES

Tags

myc tag

Expression

Mammalian

Mutation

UGI-E11K

Promoter

CMV

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR 7SK-miR-30 L1-L4

Plasmid

#62180

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only
pBa-FRB-3myc-Rab5a

Plasmid

#64209

Purpose

Rab5a with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Rab5a (Rab5a Mouse)

Tags

3myc and FRB

Expression

Mammalian

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-NmeCas9-NLS-3XHA-NLS

Plasmid

#87448

Purpose

Mammalian expression of wild type Nme Cas9

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Nme Cas9

Use

CRISPR

Tags

3XHA, SV40 NLS, and cMyc-like NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCXLE-hS + eGFP

Plasmid

#74945

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

Sox2 (SOX2 Human)

Expression

Mammalian

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf106

Plasmid

#12794

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf106

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pCMV_Anc689-nCas9

Plasmid

#163526

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

Anc689-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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