2,294 results

1. pHes1-GFP (CC#109)

   Plasmid

   #15133

   Depositor

   Connie Cepko

   Article

   Furukawa et al Neuron. 2000 May . 26(2):383-94.

   Insert

   Hes1 (Hes1 Rat)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pOC1 Halo-Actb KI

   Plasmid

   #183426

   Purpose

   CreON knock-in for Halo-beta-actin (amino acid position: before start codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and Halo donor
   

   Use

   CRISPR

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

   Plasmid

   #68720

   Purpose

   Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

   Depositor

   Tobias Bonhoeffer, Mark Huebener, Tobias Rose

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6s
   

   Use

   AAV

   Mutation

   Cre-dependent expression from inverted open readi…

   Promoter

   hSyn1-FLEX

   Available Since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Kv4.3 long/RBG4

   Plasmid

   #196946

   Purpose

   Plasmid expressing an antigen targeting the Kv4.3 potassium channel subunit

   Depositor

   James Trimmer

   Insert

   Kv4.3 (Kcnd3 Rat)
   

   Expression

   Mammalian

   Available Since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cherry-HA-KCC2

   Plasmid

   #104077

   Purpose

   To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

   Depositor

   Igor Medyna

   Article

   Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10

   Insert

   KCC2 (b isoform) (Slc12a5 Rat)
   

   Tags

   mCherry-HA tag

   Expression

   Mammalian

   Mutation

   EcoR1 site in rat KCC2 was removed by silent muta…

   Promoter

   UbC

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiviral shRNA GEF-H1

   Plasmid

   #21477

   Depositor

   Richard Huganir

   Article

   Kang et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

   Insert

   rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2 Rat)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pTrcHis-p47

   Plasmid

   #21268

   Depositor

   Hemmo Meyer

   Article

   Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

   Insert

   p47 (Nsfl1c Rat)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-CathespinB-6

   Plasmid

   #55007

   Purpose

   Localization: Lysosomes, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   CathespinB
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pORANGE GFP-CACNA1E KI

   Plasmid

   #131481

   Purpose

   Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cacna1e, Cacna1e Rat, Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBK-YE2-BE3

    Plasmid

    #85176

    Purpose

    Expresses YE2-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YEE-BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. mcherry-Myo 9b R1695M

    Plasmid

    #134958

    Purpose

    Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.

    Depositor

    Martin Bähler

    Article

    Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

    Insert

    Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. KvB2/RBG4

    Plasmid

    #196942

    Purpose

    Plasmid expressing an antigen targeting the Kvbeta2 potassium channel subunit

    Depositor

    James Trimmer

    Insert

    KvB2/B6 (Kcnab2 Rat)
    

    Expression

    Mammalian

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. miniSOG-RatLC3

    Plasmid

    #57775

    Purpose

    Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    LC3
    

    Tags

    miniSOG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pEGFP SV2A GFP

    Plasmid

    #218544

    Purpose

    Mamallian Expression of SV2A GFP

    Depositor

    Edwin Chapman

    Article

    Dong et al Science. 2006 Apr 28;312(5773):592-6. do

    Insert

    SV2A
    

    Expression

    Mammalian

    Mutation

    S42F

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBactin-Nedd1-mOrange2-hCM1

    Plasmid

    #196863

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-hCM1 (Nedd1 Human, Rat)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA

    Plasmid

    #51473

    Purpose

    Bicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.

    Depositor

    Tobias Bonhoeffer, Mark Huebener, Tobias Rose

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Insert

    mRuby2-P2A-GCaMP6m
    

    Use

    AAV

    Promoter

    hSyn1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-SypHluorin 1x (S1x)

    Plasmid

    #37003

    Depositor

    Stephen Heinemann, Yongling Zhu

    Article

    Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

    Insert

    SypHluorin (Syp Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    One copy of pHluorin inserted at site 184-185

    Promoter

    CMV

    Available Since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T-1 3xFlag-ERK2

    Plasmid

    #47573

    Purpose

    Bacterial expression plasmid for GST-3xFlag-tagged ERK2

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    ERK2 (Mapk1 Rat)
    

    Tags

    3xFlag and GST

    Expression

    Bacterial

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

19. FKBP-AP180-GFP

    Plasmid

    #186577

    Purpose

    Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    AP180 (Snap91 Rat)
    

    Tags

    FKBP N-terminal GFP C-terminal

    Expression

    Mammalian

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACT2-μ3A

    Plasmid

    #198174

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-3 μ3A
    

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    silent substitution in codon 225

    Promoter

    ADH1

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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