2,226 results

1. pcDNA3-SypHluorin 1x (S1x)

   Plasmid

   #37003

   Depositor

   Stephen Heinemann, Yongling Zhu

   Article

   Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

   Insert

   SypHluorin (Syp Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   One copy of pHluorin inserted at site 184-185

   Promoter

   CMV

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-mEGFP(A206K)-paCaMKII(T286A)

   Plasmid

   #165439

   Purpose

   Expresses photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII(T286A)
   

   Use

   Tags

   mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   T286A: Autophosphorylation deficient mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. MUNC18-2

   Plasmid

   #186687

   Purpose

   Expresses MUNC18-2 in insect cells

   Depositor

   Axel Brunger

   Article

   Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

   Insert

   MUNC18-2 (Stxbp2 Rat)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZac2.1-GfaABC1D-lck-jGCaMP8m

   Plasmid

   #176760

   Purpose

   AAV-mediated expression of membrane bound GCaMP8m under the control of GfaABC1D promoter

   Depositor

   Loren Looger

   Has Service

   AAV1 and AAV9

   Insert

   lck-jGCaMP8m
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GfaABC1D

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. KIF5C(1-560)-2xmCitrine-GFP11

   Plasmid

   #61667

   Purpose

   Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Kv2.1/RBG4

   Plasmid

   #196947

   Purpose

   Plasmid expressing an antigen targeting Kv2.1 (RBG4)

   Depositor

   James Trimmer

   Insert

   Kv2.1 (Kcnb1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mRuby-Lysosomes-20

   Plasmid

   #55872

   Purpose

   Localization: Lysosome Membrane, Excitation: 558, Emission: 605

   Depositor

   Michael Davidson

   Insert

   LAMP1
   

   Use

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. mTagBFP-Cx43-7

   Plasmid

   #55256

   Purpose

   Localization: Gap Junctions, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   mTagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pBBK90 pHomL-TEF2p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

   Plasmid

   #179074

   Purpose

   Vector containing a yeast integration cassette for expressing TEF2p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   TEF2p-Scarlet(rfp)-Link-2xPH
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-sh-Nix

    Plasmid

    #100770

    Purpose

    Lentiviral expression of shRNA targeting Nix

    Depositor

    Joseph Gordon

    Article

    da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

    Insert

    Lenti-sh-Nix
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-dCpf1-BE-YEE

    Plasmid

    #107687

    Purpose

    Expresses dLbCpf1-BE-YEE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE-YEE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y/R126E/R132E), dLbCpf1(D832A/E925A/D1…

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCIS-krPAM1

    Plasmid

    #25456

    Depositor

    Richard Mains

    Article

    Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

    Insert

    PAM1 (Pam Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

13. PKC zeta DN

    Plasmid

    #21249

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC zeta (Prkcz Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K281M

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pMK285 (Stag-3FLAG-Bsr)

    Plasmid

    #72801

    Purpose

    C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    Stag-3FLAG-Bsr
    

    Use

    CRISPR

    Tags

    Stag-3FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ratGLUT2

    Plasmid

    #14946

    Depositor

    Bernard Thorens

    Article

    Thorens et al J Biol Chem. 1996 Apr 5. 271(14):8075-81

    Insert

    GLUT2 (Slc2a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pET30-Npl4

    Plasmid

    #21267

    Depositor

    Hemmo Meyer

    Article

    Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

    Insert

    Npl4 (Nploc4 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pSLIK S6K (E389-deltaCT) neo

    Plasmid

    #58516

    Purpose

    Lentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCT

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Rps6kb1 (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T389E, stop codon at amino acid 402

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

18. AAV-hSyn-Ribo-jGCaMP8f

    Plasmid

    #167573

    Purpose

    DEPRECATED: Please see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    Ribo-GCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-SYN_mCyRFP2-CREB

    Plasmid

    #137008

    Purpose

    AAV backbone, mCyRFP2-CREB donor for R-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    AAV

    Tags

    mCyRFP2

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-IRES2-p75NTR-GFP

    Plasmid

    #182947

    Purpose

    To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal

    Depositor

    Pantelis Tsoulfas

    Insert

    p75NTR-mGFP
    

    Use

    Tags

    HA tag and mGFP

    Expression

    Mammalian

    Mutation

    The leader sequence is from preprotrypin

    Promoter

    CAG

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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