2,226 results

1. mEos2-CathespinB-6

   Plasmid

   #57354

   Purpose

   Localization: Lysosomes, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   CathespinB (Ctsb Rat)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pSM1-1-1

   Plasmid

   #15995

   Depositor

   Morris Birnbaum

   Article

   Birnbaum et al Cell. 1989 Apr 21. 57(2):305-15.

   Insert

   GLUT4 (Slc2a4 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1/HisC 14-3-3 epsilon K49E

   Plasmid

   #11943

   Depositor

   Michael Yaffe

   Article

   Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

   Insert

   Ywhae (Ywhae Rat)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   K49E

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

4. Syn2ApcDNA4/TO

   Plasmid

   #14817

   Depositor

   Thomas Weimbs

   Article

   Low et al Dev Cell. 2003 May . 4(5):753-9.

   Insert

   Syntaxin2A (Stx2 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Septin 11 isoform I

   Plasmid

   #120314

   Purpose

   Septin 11 isoform I mammalian expression

   Depositor

   Angel de Blas

   Article

   Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

   Insert

   SEPT11 (Sept11 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TSC2-S1345D

   Plasmid

   #38107

   Depositor

   Kunliang Guan

   Insert

   tsc2 (Tsc2 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   S1345D (equivalent to S1389D in rat TSC2 isoform …

   Promoter

   Available since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

7. PKC beta I dNPS

   Plasmid

   #16379

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC beta 1 (Prkcb Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N-terminal pseudosubstrate domain deletion: aa 30…

   Promoter

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pCDNA3-PKA-RIIβ-sREACh

   Plasmid

   #114113

   Purpose

   Mammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   PKA regulatory subunit RII beta tagged by sREACh (low-irradiant YFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EBFP2-CathespinB-6

   Plasmid

   #55236

   Purpose

   Localization: Lysosomes, Excitation: 383, Emission: 448

   Depositor

   Michael Davidson

   Article

   Sundaresan et al J Clin Oncol. 1987 Nov;5(11):1739-45.

   Insert

   CathespinB
   

   Use

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. mTagGFP2-Cx43-7

    Plasmid

    #56414

    Purpose

    Localization: Gap Junctions, Excitation: 483, Emission: 506

    Depositor

    Michael Davidson

    Insert

    Cx43
    

    Use

    Tags

    mTagGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc

    Plasmid

    #25458

    Depositor

    Betty Eipper

    Article

    Chufan et al Structure. 2009 Jul 15. 17(7):965-73.

    Insert

    PAM (Pam Rat)
    

    Use

    Tags

    His6 and PAM signal

    Expression

    Mammalian

    Mutation

    Contains aa 498-820 (PALcc= PAL catalytic core) …

    Promoter

    Available since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA GAL4 CREB 1-341

    Plasmid

    #22972

    Depositor

    Marc Montminy

    Article

    Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

    Insert

    CREB
    

    Use

    Tags

    GAL4 DNA-binding domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pGEX-4T Doc2beta C2AB(125) WT

    Plasmid

    #134690

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression and purification

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV0-OCT-HA-eGFP

    Plasmid

    #67479

    Purpose

    Targets HA-eGFP to mitochondrial matrix

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    OCT-HA-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. mCherry-Cx26-7

    Plasmid

    #55021

    Purpose

    Localization: Gap Junctions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Cx26
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

16. Anti-TrpV3 [N15/4R]

    Plasmid

    #177501

    Purpose

    Mammalian Expression Plasmid of anti-TrpV3 (Rat). Derived from hybridoma N15/4.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-TrpV3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP ratAPOBEC1 L173A/G227A

    Plasmid

    #167169

    Purpose

    Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Leucine 173 to Alanine; changed Glycine 2…

    Promoter

    CMV promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LXSN-Cx43-mAPPLE

    Plasmid

    #60696

    Purpose

    retroviral expression of rat Cx43 fused to mAPPLE

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    mAPPLE

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA-Cx43-YA/VD

    Plasmid

    #60699

    Purpose

    mammalian expression of rat Cx43 YA/VD mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    YA/VD (Y286A/V289D)

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pDS170-enNTS1

    Plasmid

    #199152

    Purpose

    JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)

    Depositor

    Daniel Scott

    Article

    Bumbak et al Cell Rep. 2023 Jan 31;42(1):112015. doi:

    Insert

    enNTS1 (Ntsr1 Rat)
    

    Use

    Tags

    10xHis tag, Avi tag, MBP, and muGFP

    Expression

    Bacterial

    Mutation

    V208M, C273S, C391S and C393S (over NTS1-B5)

    Promoter

    Lac/T5

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. rat APOBEC1 E63A

    Plasmid

    #112861

    Purpose

    plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Glutamic acid 63 to Alanine

    Promoter

    CMV promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LUC56 (Luc/UGA/SP)

    Plasmid

    #112147

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Photinus pyralis, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cysteine 258mutated to selenocysteine (U)

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3 Glt1a

    Plasmid

    #98705

    Purpose

    mammalian expression of Glt1a

    Depositor

    Paul Rosenberg

    Article

    Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

    Insert

    Glt1a (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I288V (please see depositor comments below)

    Promoter

    CMV

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. iLID-mCherry-iTrkA

    Plasmid

    #136509

    Purpose

    Mammalian expression of iLID fused to mCherry and intracellular signaling domain of TrkA (aa 450-799)

    Depositor

    Bianxiao Cui

    Article

    Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

    Insert

    iLID mCherry iTrkA (Ntrk1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mEos2-Homer1-N-18

    Plasmid

    #57388

    Purpose

    Localization: Membrane Adaptor, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Insert

    Homer1 (Homer1 Rat)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pCI-neo-FHM

    Plasmid

    #51871

    Purpose

    Expresses N-terminal FLAG-HA-tagged full length rat MIBP1 (cloned into pCI-neo)

    Depositor

    Tomoko Tahira, Kenshi Hayashi

    Article

    Iwashita et al J Biol Chem. 2012 Mar 23;287(13):9887-90

    Insert

    MIBP1 (Hivep2 Rat)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

27. LXSN-Cx43-YA/VD

    Plasmid

    #60700

    Purpose

    retroviral expression of rat Cx43 YA/VD mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    YA/VD (Y286A/V289D)

    Promoter

    LTR

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

28. LXSN-Cx43-S279D

    Plasmid

    #60707

    Purpose

    retroviral expression of rat Cx43 S279D mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S279D

    Promoter

    LTR

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

29. LXSN-Cx43-S282A

    Plasmid

    #60709

    Purpose

    retroviral expression of rat Cx43 S282A mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S282A

    Promoter

    LTR

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

30. LXSN-Cx43-S282D

    Plasmid

    #60711

    Purpose

    retroviral expression of rat Cx43 S282D mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S282D

    Promoter

    LTR

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only