We narrowed to 31,545 results for: Eng;
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Plasmid#126717Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTagsExpressionYeastMutationPromoterGAPAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS2362_Tier3(SB)-rtTA-PuroR
Plasmid#169658PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCHC005 (∆RE)
Plasmid#226199PurposeConjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).DepositorInsertHomology arms for type I restriction-modification system restriction subunit H16_A0006
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZ_P-AOX1-eGFP
Plasmid#126718Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHC039 (∆phcA)
Plasmid#226207PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.DepositorInsertHomology arms for PhcA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTS2303-Tier1-CoTC-PPGK-NLuc_TB
Plasmid#169548PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertCoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
PlepA
Plasmid#55175PurposeStrong constitutive promoterDepositorInsertlepA promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-cTF
Plasmid#87743PurposeIPTG-inducible expression of ligase-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and cTF (chimeric transcription factor base…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbE1k-3
Plasmid#71722Purposetyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from humanDepositorUseTagsExpressionMutationPromoterpTrcAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB3K3 containing mRFP1
Plasmid#118083PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertpromoter, RBS, mRFP1
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3 containing mRFP1
Plasmid#118082PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertpromoter, RBS, mRFP1
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_P-GAP-ACC1*
Plasmid#126740Purposeyeast plasmid overexpressing ACC1 from Komagataella phaffiiDepositorInsertacc1*
UseTagsExpressionYeastMutationT3452GPromoterGAPAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
asPink chromoprotein promoter-less
Plasmid#118005PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, asPink
UseEscherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta-mmr
Plasmid#169241PurposeCas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutationsDepositorInsertscas9
beta
mutL-E36K
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationChanged glutamic acid 36 to lysinePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
p168
Plasmid#62618PurposeePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-NpgA
Plasmid#126731Purposeyeast plasmid overexpressing NpgADepositorInsertnpgA
UseTags6xHisExpressionYeastMutationG951A,C957APromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2187
Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
UseTagsExpressionBacterialMutationPromoterlacUV5Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
PliaI
Plasmid#55177PurposeBacitracin-inducible promoterDepositorInsertliaI promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterliaIAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTS2337_Tier3(SB)-TagBFP2_Puro
Plasmid#169635PurposeTier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
PliaG
Plasmid#55174PurposeConstitutive promoterDepositorInsertLiaG promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only