We narrowed to 29,389 results for: des.2

1. pET15b+2(L171C)X

   Plasmid

   #108192

   Purpose

   Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PCNA2(L171C)-PdX fusion protein
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   L171C (PCNA2); C73S/C85S (PdX)

   Promoter

   T7 promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. AB.pCCL.sin.cPPT.GFP.miR-138-2-3p.sensor.PGK.dNGFR.WPRE

   Plasmid

   #85897

   Purpose

   Sensor Plasmid

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Inserts

   miR-138-3-3p Sesnor
   miR-138-2-3p Sensor (MIR138-2 Human)
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Integrin beta 2 - mYFP

   Plasmid

   #8638

   Depositor

   Timothy Springer

   Article

   Kim et al Science 2003 Sep 19;301(5640):1720-5.

   Insert

   Integrin beta 2 (ITGB2 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   stop codon of beta2 was replaced with AgeI restri…

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMP71-super-IL-2

   Plasmid

   #174599

   Purpose

   Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Bcl-2-ΔBH4

   Plasmid

   #220300

   Purpose

   mammalian expression of Bcl-2-ΔBH4 tagged with GFP

   Depositor

   Xinlai Cheng

   Article

   Wang et al Cell Reports Physical Science, Volume 5,

   Insert

   Bcl2 (BCL2 Human)
   

   Tags

   Emerald GFP

   Expression

   Mammalian

   Mutation

   BH4 deleted

   Promoter

   CMV

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-GSK3β-#2

   Plasmid

   #32497

   Depositor

   Alex Toker

   Article

   Yoeli-Lerner et al Mol Cancer Res. 2009 Mar;7(3):425-32. Ep

   Insert

   GSK3B (GSK3B Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

7. Human Integrin beta 2

   Plasmid

   #8640

   Depositor

   Timothy Springer

   Article

   Kishimoto et al Cell 1987 Feb 27;48(4):681-90.

   Insert

   Integrin beta 2 (ITGB2 Human)
   

   Expression

   Mammalian

   Available Since

   June 23, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pGEX-αTAT1[2-236]

   Plasmid

   #27101

   Depositor

   Maxence Nachury

   Article

   Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

   Insert

   αTubulin K40 acetyltransferase (ATAT1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX SH3(2)-4R

   Plasmid

   #112090

   Purpose

   Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   SH3(2)-4R (NCK1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX SH3(2)-3R

    Plasmid

    #112091

    Purpose

    Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-3R (NCK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaRIgG-2

    Plasmid

    #228996

    Purpose

    For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaRIgG-2
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRetrosuper Fbw7 shRNA-2

    Plasmid

    #15661

    Depositor

    Martin Eilers

    Article

    Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

    Insert

    Fbw7 shRNA (FBXW7 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

13. YFP-DAXX-mSIM1/2

    Plasmid

    #134525

    Purpose

    Expresses YFP-DAXX fusion protein with a mutations in both its 5' and 3' SIMs

    Depositor

    Susan Janicki

    Article

    Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs

    Insert

    DAXX-mSIM1/2
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    SIM (IIVL) 7-10 aa changed to IGAG and SIM (IIVL)…

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CoV-2-NSP3-c001

    Plasmid

    #173083

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E1084-E1192, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CoV-2-NSP3-c002

    Plasmid

    #173084

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
    

    Expression

    Bacterial

    Mutation

    E1084-E1192, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXPR_003 sgMYC guide 2

    Plasmid

    #193597

    Purpose

    cMYC knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgMYC guide 2 (MYC Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLuc-CDS-m215-2

    Plasmid

    #42102

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    ZEB1 (ZEB1 Human)
    

    Use

    Luciferase

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Silent mutations were introduced in the seed-comp…

    Promoter

    SV40

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. MDH1-shDusp5-2-H2Kb2

    Plasmid

    #17853

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    shDusp5 (DUSP5 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO-shAUF1 hairpin 2

    Plasmid

    #52922

    Purpose

    expresses a hairpin specific for human AUF1

    Depositor

    Sheila Stewart

    Article

    Alspach et al Cancer Discov. 2014 Mar 26.

    Insert

    shRNA targeting AUF1 (HNRNPD Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pLG1-puro-sgATL3-2

    Plasmid

    #109010

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #2 (ATL3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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