We narrowed to 14,422 results for: nts

1. pET21c(+)-mtLeuRS

   Plasmid

   #31079

   Depositor

   Linda Spremulli

   Article

   Bullard et al Biochim Biophys Acta. 2000 Feb 29. 1490(

   Insert

   Mature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes coding sequence for the mature form. Nt …

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

   Plasmid

   #205268

   Purpose

   Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1 omegaRNA
   

   Expression

   Mammalian

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNL4-3 envFS eGFP Δvif/vpr

   Plasmid

   #101344

   Purpose

   Deletion from NL4-3 nt 5582-6199 encompassing Vif and Vpr coding sequence

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Mutation

   delta vif/Vpr

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-mCherry-CyrCR1

   Plasmid

   #128768

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. hum sgk promoter F4R4

   Plasmid

   #83426

   Purpose

   promoter reporter construct to test 5' flanking sequences

   Depositor

   Christie Thomas

   Article

   Itani et al Am J Physiol Endocrinol Metab. 2002 Nov;

   Insert

   human Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Promoter

   sgk

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. ptagRFP-MS2coatProtein

   Plasmid

   #103831

   Purpose

   Live cell reader for MS2 loop RNA production

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   MS2 coat protein (MCP) (cp )
   

   Tags

   tagRFP

   Expression

   Mammalian

   Mutation

   MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

   Promoter

   CMV

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

   Plasmid

   #205278

   Purpose

   Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1 omegaRNA
   

   Expression

   Mammalian

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Fireworks RFP[PTC-] (AVA2515)

   Plasmid

   #85443

   Purpose

   Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

   Depositor

   Joan Steitz

   Article

   Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

   Insert

   tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
   

   Use

   Minimal backbone fragment for low-copy bacterial …

   Expression

   Mammalian

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-dSa-VPR mini.-2X snRP1

   Plasmid

   #99688

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Mutation

   dead Cas9

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmKate2-MS2coatProtein

    Plasmid

    #103833

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    mKate2-MS2 coat protein (cp )
    

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiMutB3GALT5-blast

    Plasmid

    #208381

    Purpose

    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nucleotide change in PAM targeting sequence nt 51…

    Promoter

    EF-1 alpha core promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiMutB3GALT5catdead-blast

    Plasmid

    #220869

    Purpose

    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    encodes D156A and D243A mutations; also, nucleoti…

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-V96 ELP

    Plasmid

    #67853

    Purpose

    GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

    Depositor

    Andrew MacKay

    Article

    Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

    Insert

    GFP fused to ELP (VPGVG) repeated 96 times
    

    Tags

    GFP fused to N-terminal of ELP

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMJ162: pU6-SpuFz1_omegaRNA(75nt)-entry

    Plasmid

    #205261

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS NL4-3 envFS eGFP Δnef/U3

    Plasmid

    #101346

    Purpose

    Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequences

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Mutation

    delta nef/LTR

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-nDsRedFL-miRNT

    Plasmid

    #177808

    Purpose

    A nontargeting miRNA based on the pre-miR- 30a structure (Qiu et al., 2008) has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-NT
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL1.7XX

    Plasmid

    #49344

    Purpose

    luciferase reporter with mouse Bmp2 promoter

    Depositor

    Melissa Rogers

    Article

    Abrams et al J Biol Chem. 2004 Apr 16;279(16):15916-2

    Insert

    Bmp2 distal promoter (Bmp2 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Mutation

    nt –1237 to +471

    Promoter

    Bmp2 distal promoter

    Available Since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. MCV-Rep-

    Plasmid

    #32058

    Depositor

    Patrick Moore

    Article

    Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

    Insert

    MCV-Rep- genome
    

    Mutation

    Single cytosine to adenine (C/A) mutation at 2,07…

    Promoter

    none from vector. Insert contains native promoter…

    Available Since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)

    Plasmid

    #191801

    Purpose

    Full length wt AtPCO4 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO4-1
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pChlr-8CQ

    Plasmid

    #52171

    Purpose

    Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    

    Expression

    Bacterial

    Mutation

    Changed 12N to 12C in module 8

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only