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pEMS1199

Plasmid

#29100

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple53 (DCX Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 22, 2011

Availability

Academic Institutions and Nonprofits only

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CMV ArcLightCo (Q239)-T2A-nls-mCherry

Plasmid

#85806

Purpose

Genetically encoded voltage sensor ArcLight- codon optimized

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

ArcLightCo-Q239

Tags

mCherry

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

Plasmid

#83484

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pC1-CMV-DAAO-NES

Plasmid

#141107

Purpose

Expresses DAAO with nuclear export signal in mammalian cells

Depositor

Vsevolod Belousov

Article

Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

Insert

DAAO-NES

Tags

Nuclear export signal

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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LUC01 (L/Sec/P)

Plasmid

#112144

Purpose

Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

Depositor

Paul Copeland

Article

Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

Insert

Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)

Expression

Mammalian

Mutation

Cysteine 258 mutated to selenocysteine (U)

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pIYC04

Plasmid

#64741

Purpose

Yeast expression plasmid

Depositor

Jens Nielsen

Article

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgMYC

Plasmid

#125770

Purpose

constitutive expression of a guide RNA targeting human MYC (CRISPR positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgMYC (MYC Human)

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSLED.ENS

Plasmid

#193016

Purpose

Excitatory neuron-specific reporter (hSyn promoter, Synrg exon, bichromatic reporter)

Depositor

Jonathan Ling

Article

Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

Insert

Bichromatic reporter (EGFP and dsRed-Express2)

Use

AAV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shWRN1

Plasmid

#125781

Purpose

constitutive expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1 (WRN Human)

Use

RNAi

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-Puro-CLEC16A-EMP2

Plasmid

#69468

Purpose

Expresses CLEC16A-EMP2 fusion gene

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

CLEC16A-EMP2 (CLEC16A, EMP2 Human)

Use

Retroviral

Tags

HA TAG

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-HIF2a dPA (P405A, P531A)

Plasmid

#232954

Purpose

Stably express a constitutively stable version of HIF2a (HIF2a dPA)

Depositor

William Kaelin

Article

Jiang et al Cell. 2025 Feb 25:S0092-8674(25)00156-4.

Insert

HIF2a (EPAS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P405A, P531A

Promoter

EF1a

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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Ai167 (TIT2L-ChrimsonR-tdT-ICL-tTA2) targeting vector

Plasmid

#114431

Purpose

Target a Cre-dependent ChrimsonR-tdTomato cassette and a tTA2 cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

ChrimsonR-tdTomato, tTA2

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Ai170 (TIT2L-ASAP2s-Kv-ICL-tTA2) targeting vector

Plasmid

#114435

Purpose

Target a Cre-dependent voltage sensor ASAP2s cassette into the mouse TIGRE locus

Depositor

Michael Lin, Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

ASAP2s-Kv, tTA2

Use

Cre/Lox and Mouse Targeting

Tags

Kv2.1 tag

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pTH733-2µ-RLuc/maxCFLuc

Plasmid

#40608

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pAP05

Plasmid

#186261

Purpose

sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

Depositor

Yongku Cho

Article

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Inserts

sfGFP
EL222 (ELI_04755 Erythrobacter litoralis)

Expression

Bacterial

Promoter

BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-spCas9

Plasmid

#231404

Purpose

Expresses spCas9 from hSyn promoter

Depositor

Arjen Boender, Larry Young

Article

Schimmer et al Nat Commun. 2026 Feb 20;17(1):1932. doi:

Insert

hSYN promoter (RIC8B Human)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

hSYN

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-METTL14-WT

Plasmid

#141118

Purpose

Retroviral overexpression of WT METTL14 in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

METTL14 (METTL14 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 scramble (plus strand)

Plasmid

#91852

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0347)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 scramble (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Sequence scrambled from ch10:6094588-6094934

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 (minus strand)

Plasmid

#91849

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0626)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE3 (plus strand)

Plasmid

#91844

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0621)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE3 (plus strand) (CD69 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Missing 1 base from 13 base polyT tract beginning…

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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