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We narrowed to 10,556 results for: aav

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Showing: 8981 - 9000 of 10556 results

  4. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

    Plasmid
    #170182
    Purpose
    Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide
    Depositor
    Hunter Fraser
    Publication
    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Tags
    3XFlag and T2A
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    170182-plasmid-map-sequence-id-333578

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  5. pX335-NQL002-WAPL-sgRNA1

    Plasmid
    #175550
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL003-WAPL-sgRNA2 targeting construct.
    Depositor
    Elzo de Wit
    Publication
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nickase and sgRNA against mouse WAPL STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    175550-plasmid-map-sequence-id-345078

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  6. pX335-NQL003-WAPL-sgRNA2

    Plasmid
    #175551
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL002-WAPL-sgRNA1 targeting construct.
    Depositor
    Elzo de Wit
    Publication
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nickase and sgRNA against mouse WAPL STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    175551-plasmid-map-sequence-id-345081

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  8. pX330_Actb sgRNA / hSpCas9

    Plasmid
    #172832
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172832-plasmid-map-sequence-id-340914

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  9. pX330_TOMM20 sgRNA / hSpCas9

    Plasmid
    #172836
    Purpose
    Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172836-plasmid-map-sequence-id-340903

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  12. pJRH056

    Plasmid
    #171637
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Publication
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-tdTomato
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171637-plasmid-map-sequence-id-354439

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  17. pspCas9-ABE-3'UTR-sgRNA-2xMS2

    Plasmid
    #132554
    Purpose
    Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2
    Depositor
    Baisong Lu
    Publication
    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABEmax
    sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
    Expression
    Mammalian
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    132554-plasmid-map-sequence-id-259533

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  20. (364) SB KRAB-SadCas9

    Plasmid
    #163022
    Purpose
    Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)
    Depositor
    Tonia Rex
    Publication
    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mammalian codon-optimized SadCas9
    Use
    Transposon
    Tags
    KRAB and myc NLS
    Expression
    Mammalian
    Promoter
    TET-On
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163022-plasmid-map-sequence-id-320905

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Showing: 8981 - 9000 of 10556 results