We narrowed to 23,118 results for: Sis

Showing: 9021 - 9040 of 23118 results

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pCS-H2B-EYFP

Plasmid

#53746

Purpose

contains fusion of human histone H2B to EYFP in pCS2+ vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

EYFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only
pDEST_6xUAS:miRFP670-cetn4

Plasmid

#192357

Purpose

zfCETN4 tagged with miRFP670, expressed under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

CETN4 (cetn4 Zebrafish)

Tags

miRFP670

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pI3-MTU-DO-G418

Plasmid

#160023

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSicoR human DGCR8-1

Plasmid

#14769

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (DGCR8 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
p2T-CMV-eA3AmaxNG-BlastR

Plasmid

#152999

Purpose

C-to-T base editor, NG PAM

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-nSpCas9 NG-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRU293

Plasmid

#167688

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe hygro NFS1

Plasmid

#102977

Purpose

Expression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

NFS1 (NFS1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Silent mutations to eliminate shNFS1_1 binding si…

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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HBT_pcoCASphi_version2

Plasmid

#197932

Purpose

Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Tags

2xNLS-2xFLAG

Expression

Plant

Promoter

35SPPDK promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-Ufm1 WT

Plasmid

#133865

Purpose

Expression of human wild type UFM1

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

Ubiquitin-fold modifier 1 (UFM1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-26

Plasmid

#120005

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mAID-Clover

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mAID-Clover

Use

Pcr template

Tags

mAID-Clover

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P06

Plasmid

#120017

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with sfGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

sfGFP

Use

Pcr template

Tags

sfGFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-CR7T-gRNA2.1-nlsBFP

Plasmid

#170515

Purpose

gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

CR7T

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1354

Plasmid

#69490

Purpose

Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

Depositor

Jordan Ward

Article

Zhang et al Development. 2015 Nov 9. pii: dev.129635

Insert

GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope

Use

CRISPR; Template to generate cassettes for -media…

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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eGFP-CBP

Plasmid

#169211

Purpose

Expression of eGFP-CBP protein for chitin visualization

Depositor

James S Fraser

Article

Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

Insert

Chitinase A1 Chitin Binding Domain

Tags

6xHis, Mxe Gyr Intein, and eGFP

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-Pidd1(T788A)

Plasmid

#60529

Purpose

Retroviral expression of Pidd1 T788A phosphorylation mutant

Depositor

Trudy Oliver

Article

Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

Insert

Pidd1 (PIDD1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Threonine 788 to Alanine*

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only
pUPD2_miniDFR (GB2566)

Plasmid

#160595

Purpose

Minimal promoter of slDFR gene containing 62bp upstream the transcription start site and the 5'UTR region

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_C'5802

Plasmid

#176879

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10:Luc+:RbcsE9

Use

Luciferase

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET-BoNT/E

Plasmid

#168020

Purpose

Expresses BoNT/E-LC(1-411)-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

BoNT/E-LC(1-411)-6xHis

Tags

Hisx6

Expression

Bacterial

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-squirrel-monkey-retroCHMP3-HA

Plasmid

#154174

Purpose

expresses squirrel monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pnxABE-flgn

Plasmid

#187932

Purpose

A-to-G base editor

Depositor

Yi Xiao

Article

et al ACS Synth Biol. 2022 Apr 15;11(4):1650-1

Insert

ecTadA(8e)-nxCas9 (D10A)

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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