We narrowed to 23,637 results for: promoter

1. pUC19-YAP-mEGFP

   Plasmid

   #166472

   Purpose

   HDR template for tagging of endogenous mouse YAP N-terminus with mEGFP

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   YAP-mEGFP HDR template(mouse Yap) (Yap1 Mouse)
   

   Expression

   Mammalian

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6-sgR26-eCas9-T2A-BlastR

   Plasmid

   #172493

   Purpose

   For CRISPR-assisted HDR, Rosa26 locus

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Insert

   eCas9, BlastR and sgRNA targeting the Rosa26 (R26) safe harbor locus
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

   Plasmid

   #112214

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   DNMT3L-L-DNMT3A-L-dcas9 (DNMT3L, DNMT3A Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Mutation

   D10A,H840A,N863A

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-Neo-GFP/Foxa1

   Plasmid

   #105506

   Purpose

   MSCV-driven retroviral Foxa1 expression

   Depositor

   Christopher Vakoc

   Article

   Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

   Insert

   Foxa1 (Foxa1 Mouse)
   

   Use

   Retroviral

   Available Since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXPR_sgFLNB-SK2

   Plasmid

   #119873

   Purpose

   gRNA expression vector

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   sgFLNB-SK2 (FLNB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXPR_sgFLNB-SK4

   Plasmid

   #119874

   Purpose

   gRNA expression vector

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   sgFLNB-SK4 (FLNB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLIK_Hyg_mTurquoise2_Atg4BC74A

   Plasmid

   #161733

   Purpose

   Doxycycline-inducible expression of Atg4B(C74A) fused with mTurquoise2 to inhibit autophagy

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   220-222 TGC (Cys) is changed to GCA (Ala)

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLZRS-ER-Rac1-Q61L-shortlinker

   Plasmid

   #128584

   Purpose

   For mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   Modified hormone binding domain of ER alpha

   Expression

   Mammalian

   Mutation

   Changed Glutamine61 to Leucine in the RAC1 moiety.

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLZRS-ER-Rac1-P29S-shortlinker

   Plasmid

   #128583

   Purpose

   For mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   Modified hormone binding domain of ER alpha

   Expression

   Mammalian

   Mutation

   Changed Proline29 to Serine in the RAC1 moiety.

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSt1374-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

    Plasmid

    #112210

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNA methyltransferase 3 alpha (DNMT3A, DNMT3L Human)
    

    Use

    CRISPR

    Mutation

    D10A,H840A,N863A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSt1374-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

    Plasmid

    #112209

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3L, DNMT3A Human)
    

    Use

    CRISPR

    Mutation

    D10A,H840A,N863A,C710S

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SITS-mCherry-Bin1b

    Plasmid

    #176025

    Purpose

    Leishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSU

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Leishmania cell free expression

    Tags

    mCherry

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196076

    Purpose

    (Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cavin4-mCherry

    Plasmid

    #176007

    Purpose

    Mammalian expression of Mouse Cavin4-mCherry. Parton lab clone JSG

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Cavin4 (Cavin4 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTEE

    Plasmid

    #159748

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-CRISPR-v2-gYap-1

    Plasmid

    #166481

    Purpose

    Expresses spCas9 and a gRNA targeting mouse Yap

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    gRNA for mouse Yap (Yap1 Mouse)
    

    Use

    Lentiviral

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBFC0694

    Plasmid

    #186693

    Purpose

    ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_lacZ_α_1 gRNA, GmR+sfGFP cargo

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsB
    tnsC
    tniQ
    Cas12k
    Sh_lacZ_α_1 gRNA
    Tn7 transposon
    GmR+sfGFP ShDART Cargo
    

    Expression

    Bacterial

    Promoter

    PLac

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Syn-FLEX-fd [Chronos-GFP]

    Plasmid

    #84482

    Purpose

    AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    human synapsin promoter

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEGFP-N1-YAPS127A-deltaCC

    Plasmid

    #166459

    Purpose

    Expresses fusion of mEGFP and YAPS127A-deltaCC (delta298-359)

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, YAPS127A-deltaCC (YAP1 Human)
    

    Expression

    Mammalian

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MIGR1-Cdc42-1

    Plasmid

    #179517

    Purpose

    Retroviral expression of Cdc42 splice variant 1

    Depositor

    Bo Porse

    Article

    Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

    Insert

    Cdc42 splice variant 1 cDNA (CDC42 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Promoter

    LTR

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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