We narrowed to 168,189 results for: Gene

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pCAG-GBP6-10gly-VPminx4

Plasmid

#50796

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP6-VPminx4 activation domain fusion protein

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_CAG-TurboGFP

Plasmid

#229781

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TurboGFP

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pJMP8930

Plasmid

#220905

Purpose

MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMB3-B-TelR

Plasmid

#204992

Purpose

Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHS-435

Plasmid

#195470

Purpose

Lentiviral Integratable Constitutive super IL-2 cytokine

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pEF1a-Super IL-2-2A-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-rTetRDBD-GBP1

Plasmid

#50791

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

rTetR DNA binding domain-GBP1 fusion protein

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ-dpcoCas9-TV

Plasmid

#158409

Purpose

CRISPR-dpcoCas9 fused with TV activator for transcriptional activation

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dpcoCas9-TV(6xTAL-2xVP64)

Use

CRISPR

Expression

Plant

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG2

Plasmid

#112814

Purpose

gateway cloning destination vector for expressing enhancer driven CD4-tdGFP

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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PGK+ episome

Plasmid

#170041

Purpose

PGK+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

PGK-tetO2

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-ZETAipi

Plasmid

#53284

Purpose

Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and pds (crtP) gene of Synechococcus PCC7942, and thereby produces zeta-carotene in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

pds

Use

Low copy number bacterial cloning vector

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pKB01-PssbB-mKate2

Plasmid

#69105

Purpose

Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mKate2

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-mCherry

Plasmid

#164426

Purpose

AAV plasmid expressing mCherry in photoreceptors

Depositor

Connie Cepko

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

Nov. 20, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-Otx2-IRES-GFPd2

Plasmid

#73996

Purpose

Plasmid expressing Otx2(isoform b)-IRES-GFPd2

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Otx2(isoform b)-Ires-GFP (Otx2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAmreB

Plasmid

#149654

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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CMV+ episome

Plasmid

#169742

Purpose

CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd6

Plasmid

#42258

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 6 (Fzd6 Mouse)

Expression

Mammalian

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
mOtop2_pcDNA3

Plasmid

#114678

Purpose

This construct was used to express mouse Otop2 in HEK-293 cells

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 2 (Otop2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCsC_spacer

Plasmid

#136077

Purpose

Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsE_spacer

Plasmid

#136078

Purpose

Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.T7 NFAT3

Plasmid

#28223

Depositor

Sebastien Jauliac

Article

Fougere et al Oncogene. 2010 Apr 15. 29(15):2292-301.

Insert

human NFAT3 (NFATC4 Human)

Tags

T7

Expression

Mammalian

Mutation

G160A

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only