We narrowed to 12,197 results for: 112

1. pQE60-mPU.1-ETS

   Plasmid

   #85733

   Purpose

   Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4

   Depositor

   Gregory Poon

   Article

   Poon et al J Biol Chem. 2012 May 25;287(22):18297-3

   Insert

   ETS domain of transcription factor PU.1 (Spi1 Mouse)
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM60-S6K1(T389S) barcoded

   Plasmid

   #48800

   Purpose

   expresses mutant S6K1

   Depositor

   David Sabatini

   Article

   Kang et al Science. 2013 Jul 26;341(6144):1236566.

   Insert

   S6K1 T389S (Rps6kb1 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG and barcode: GGTACC

   Expression

   Mammalian

   Mutation

   T389S

   Promoter

   CMV

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pCAG-Trex2-bpA-EF1BFP

   Plasmid

   #111144

   Purpose

   Expression of mouse Trex2 and BFP reporter in mammalian cells

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Inserts

   mouse Trex2 coding region (Trex2 Mouse)
   BFP (Rnf112 )
   

   Expression

   Mammalian

   Promoter

   CAG and EF1

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C2_3X Flag

   Plasmid

   #194161

   Purpose

   Expresses eGFP fluorescent protein with a 3X flag tag

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   GFP with 3X Flag
   

   Tags

   3x Flag

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. shCTL-mlpx-puro

   Plasmid

   #65232

   Purpose

   encodes a non-targeting control shRNA

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   non-coding shRNA
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs_SFXN1

   Plasmid

   #110635

   Purpose

   Retroviral expression of SFXN1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Science. 2018 Nov 16;362(6416). pii: 362

   Insert

   SFXN1 (SFXN1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   codon-optimized cDNA (no amino acid mutations)

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

   Plasmid

   #115139

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver, Keith Joung

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA3.1 anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CoV2-N-D377Y

   Plasmid

   #177957

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D377Y). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (D377Y) (N )
   

   Expression

   Mammalian

   Mutation

   D377Y

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-HA-mIFITM3-K24,83,88,104A

   Plasmid

   #58408

   Purpose

   Expresses murine IFITM3-K24,83,88,104A with an N-terminal HA tag in mammalian cells (Lysine-less nonubiquitinated mutant)

   Depositor

   Howard Hang, Jacob Yount

   Article

   Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

   Insert

   IFITM3 (Ifitm3 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Lysines 24, 83, 88, and 104 to Alanine

   Promoter

   CMV IE

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Zebrafish PAC (fPAC)

    Plasmid

    #129498

    Purpose

    Expresses zebrafish TMEM206. In pcDNA3.1(+) backbone

    Depositor

    Zhaozhu Qiu

    Article

    Yang et al Science. 2019 Apr 26;364(6438):395-399.

    Insert

    zebrafish PAC (pacc1 Zebrafish)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFOSGE_DUM1H01

    Plasmid

    #71090

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    nej (nej Fly)
    

    Use

    Gateway entry vector

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

    Plasmid

    #193915

    Purpose

    Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Farnesylation signal sequence and Flag tag

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJM240 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-aacC1)

    Plasmid

    #110561

    Purpose

    miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    stRBS-aacC1
    

    Expression

    Bacterial

    Promoter

    rhaSR-PrhaBAD

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCru5-/UAA-mEGFP-IRES-mCherry

    Plasmid

    #49225

    Purpose

    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Retroviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Promoter

    Viral LTR (or CMV)

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCerulean3-CD36-C-10

    Plasmid

    #55405

    Purpose

    Localization: Membrane, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    CD36 (CD36 Human)
    

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pFL-SV40-Rac1 5'-UTR

    Plasmid

    #115354

    Purpose

    firefly luciferase (Fluc) reporter containing the 5’-UTR of Rac1

    Depositor

    Ming-Chih Lai

    Article

    Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

    Insert

    Rac1 5'-UTR (RAC1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO-shcIAP1-B

    Plasmid

    #44131

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    c-IAP1 (BIRC2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

18. DT034A

    Plasmid

    #159395

    Purpose

    Constitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
    

    Use

    Synthetic Biology

    Tags

    FLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…

    Expression

    Bacterial

    Promoter

    J23117 and pLux promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

    Plasmid

    #115140

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver, Keith Joung

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA3 anti-CRISPR protein
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

    Plasmid

    #115138

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver, Keith Joung

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA2 anti-CRISPR protein
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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