We narrowed to 9,399 results for: pho;

1. AdMKP-3 S159AS197A

   Plasmid

   #131523

   Purpose

   Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

   Depositor

   Haiyan Xu

   Article

   Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

   Insert

   Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
   

   Use

   Adenoviral

   Tags

   No

   Mutation

   changed Serine 159 to Alanine and Serine 197 to A…

   Promoter

   CMV

   Available Since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLuc-OptoJNKi

   Plasmid

   #89744

   Purpose

   Expression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. KC1G3_HUMAN_D0

   Plasmid

   #79685

   Purpose

   This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KC1G3 (CSNK1G3 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. Lrrc4c-AP-His

   Plasmid

   #71959

   Purpose

   Expresses the extracellular region of the LRRC4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Lrrc4c (Lrrc4c Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEos2-Farnesyl-5

   Plasmid

   #57375

   Purpose

   Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson, Catherine Galbraith

   Article

   Jaqaman et al Mol Biol Cell. 2016 May 15;27(10):1561-9

   Insert

   Farnesyl (HRAS Human)
   

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. PA-mCherry-miniSOG-Keratin-17

   Plasmid

   #57792

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Keratin (KRT18 Human)
   

   Tags

   PA-mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. PA-mCherry-miniSOG-Alpha-Actinin-19

   Plasmid

   #57782

   Purpose

   Localization: Alpha Actinin, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Alpha-Actinin (ACTN1 Human)
   

   Tags

   PA-mCherry-miniSOG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

8. PA-mCherry-miniSOG-EB3-7

   Plasmid

   #57788

   Purpose

   Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   EB3 (MAPRE3 Human)
   

   Tags

   PA-mCherry-miniSOG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET21a-MKP7_wild_153

   Plasmid

   #37792

   Depositor

   SeWon Suh

   Article

   Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

   Insert

   Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   deleted amino acids 154-665

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pSecUAG-Evol2

    Plasmid

    #163148

    Purpose

    Expresses machinery necessary for selenocysteine incorporation at UAG codons

    Depositor

    Dieter Söll

    Article

    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

    Inserts

    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    

    Expression

    Bacterial

    Mutation

    Cysteine 32 switched to Selenocysteine (UAG), Cys…

    Promoter

    EM7, araBAD, native As SelD promoter, and native …

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA

    Plasmid

    #216318

    Purpose

    Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    split cerulean fluorescent protein + splice donor site
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. LPAR4-DuET

    Plasmid

    #213331

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    LPAR4 (LPAR4 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

    Plasmid

    #101060

    Purpose

    AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

    Plasmid

    #140020

    Purpose

    Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

    Promoter

    EF1

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-β2AR-μDpTT

    Plasmid

    #182326

    Purpose

    Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    beta2AR-μDronpa-TagRFP-T (ADRB2 Human)
    

    Tags

    Full-length human beta2 adrenergic receptor

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. LPAR2-DuET

    Plasmid

    #213330

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    LPAR2 (LPAR2 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLCO2A1

    Plasmid

    #131949

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLCO2A1 (SLCO2A1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAcGP67A-TfR

    Plasmid

    #12130

    Depositor

    Pamela Bjorkman

    Article

    Lebron et al Cell. 1998 Apr 3. 93(1):111-23.

    Insert

    transferrin receptor (TFRC Human)
    

    Tags

    6X-His tag, Factor Xa cleavage site, and leader p…

    Expression

    Insect

    Mutation

    The portion of the human TfR gene encoding residu…

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pET28b-ptetO::bac::gfp

    Plasmid

    #217882

    Purpose

    expresses the bac-BGC in bacterial cells, produces bacillamide D

    Depositor

    Tobias A. M. Gulder

    Article

    Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

    Inserts

    Dehydrogenase
    Non-ribosomal peptide synthetase
    Aminotransferase
    

    Expression

    Bacterial

    Promoter

    ptetO

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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