169,217 results
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Plasmid#132771PurposeInteracts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.DepositorInsertCyclic GMP-AMP synthase (CGAS Human)
UseLentiviralTagsmCherry2ExpressionMammalianMutationE225A D227AAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
BbZIP-cALFA
Plasmid#160498PurposeFor the protein expression of BbZIP with ALFA tag at C-terminus in E. coliDepositorInsertBbZIP
TagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV enAsCas12f-HKRA-sgRNA_v7
Plasmid#208357PurposeExpression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cellsDepositorInsertenAsCas12f-HKRA
ExpressionMammalianMutationI123H/D195K/D208R/V232APromoterCMVAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CSF1R-V5/HIS
Plasmid#201984Purposeexpression of human CSF1R receptor tyrosine kinase in mammalian cellsDepositorInsertCSF1R receptor tyrosine kinase (CSF1R Human)
TagsV5, 6xHisExpressionMammalianPromoterCMVAvailable SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
PylRS-AS
Plasmid#137908PurposeFor incorporation of fluorophenylalanine derivativesDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialMutationContaining N346A, C348S mutationsPromoterpBADAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mGreenLantern
Plasmid#164459PurposeLocalization of mGreenLantern fluorescent protein to actinDepositorInsertmGreenLantern
TagsLifeActExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPS
Plasmid#85801PurposeBackbone for pooled cloning of Perturb-seq librariesDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFC331
Plasmid#87843PurposeAMA1 plasmid with Aspergillus optimized Cas9 and argB selection markerDepositorInsertsCas9
argB
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and native pro…Available SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-eGFP
Plasmid#221710PurposeExpression of eGFP in zebrafish embryos.DepositorInserteGFP
UseFish expressionAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Aquamarine-LC3B-TdLanYFP
Plasmid#228560PurposeEncodes the LC3B biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoterDepositorAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only