159,258 results

1. pPS22 (pET23a-Cas10/Csm/csm2H6N)

   Plasmid

   #70038

   Purpose

   Bacterial expression of the S. epidermidis Cas10·Csm complex

   Depositor

   Luciano Marraffini

   Article

   Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

   Insert

   Cas10/Csm complex
   

   Use

   Tags

   6xHIS on N terminus of Csm2

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. FU-H2B-GFP-IRES-Puro

   Plasmid

   #69550

   Purpose

   Lentiviral, selectable fluorescent GFP tagging of nuclei.

   Depositor

   Charles Gersbach

   Insert

   Histone 2B - green fluorescent protein fusion
   

   Use

   Lentiviral

   Tags

   GFP fusion

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL-PUM1

   Plasmid

   #120384

   Purpose

   Bacterial expression of MBP-Pumillio1 fusion protein

   Depositor

   Igor Ulitsky

   Article

   Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

   Insert

   PUM1 (PUM1 Human)
   

   Use

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   LacI

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSKDuet01

   Plasmid

   #12172

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   NpuDnaE
   

   Use

   Tags

   His-GB1

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. mTagBFP2-TOMM20-N-10

   Plasmid

   #55328

   Purpose

   Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   TOMM20 (TOMM20 Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. lentiCRISPR - EGFP sgRNA 1

   Plasmid

   #51760

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLminP_Luc2P_RE4

   Plasmid

   #90338

   Purpose

   NRF2 - Antioxidant response element gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   NRF2

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2)

   Plasmid

   #169192

   Purpose

   To express SARS-CoV-2 Papain-like protease in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   6His-TEV-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Unspecified

   Tags

   6His-TEV

   Expression

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC_N_ELF2

   Plasmid

   #187737

   Purpose

   MAC-tagged gene expression of human ELF2

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   ELF2 (ELF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPet-His

    Plasmid

    #14031

    Depositor

    Patrick Daugherty

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

    Insert

    YPet
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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