We narrowed to 12,197 results for: 112

1. pLKO-Cre sgAdam10 v3

   Plasmid

   #158034

   Purpose

   lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   Adam10 sgRNA
   

   Expression

   Mammalian

   Available Since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherry

   Plasmid

   #49228

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Promoter

   EF1-short

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTV-mHdac1-tdiRFP670-PEST2

   Plasmid

   #122351

   Purpose

   Targeting vector for mouse Hdac1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   2A-tdiRFP-PEST with homology arms for Hdac1
   

   Use

   Mouse Targeting

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKP115 (pRS406-YBR139Wp-YBR139W-GFP-ADH1t)

   Plasmid

   #106465

   Purpose

   Expresses Atg42/Ybr139w with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W
   

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Promoter

   YBR139W

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG

   Plasmid

   #129758

   Purpose

   Dox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

   Insert

   ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Glutamic acid 36 to Alanine and Leucine 3…

   Promoter

   TRE3Gs and Tet inducible promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV10/3xflag-Clock V315R

   Plasmid

   #47342

   Purpose

   Mutation used for co-IPs

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   V315R

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCMV10/3xflag-Clock W284A

   Plasmid

   #47341

   Purpose

   Mutation used for co-IPs

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   W284A

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-myc NES- mCE(K294A)

   Plasmid

   #82464

   Purpose

   Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   NES mRNA capping enzyme (Rngtt HIV, Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   K294A

   Promoter

   CMV

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTV-Met-Eif4a1-tdiRFP670-PEST

   Plasmid

   #122371

   Purpose

   Targeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   2A-tdiRFP-PEST-2A with homology arms for Eif4a1
   

   Use

   Mouse Targeting

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. px335-sgRNA-EF(Cas9n)-Eif4a1-L

    Plasmid

    #122346

    Purpose

    Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Eif4a1
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. KHBD00637

    Plasmid

    #39704

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG6794 (Dif Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid

    #133347

    Purpose

    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_cpaA and sgRNA_blaA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBT2-4xUAS:P2A-mRFP

    Plasmid

    #127593

    Purpose

    This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

    Depositor

    Longhou Fang

    Article

    Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMflPT-o4

    Plasmid

    #101315

    Purpose

    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Inserts

    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    pac resistance gene
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMflST-o4

    Plasmid

    #101316

    Purpose

    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Inserts

    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    aadA1 resistance gene
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. KHBD00717

    Plasmid

    #39745

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG12124 (mxc Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pTXB1-xlH2B(1-114)_K114A

    Plasmid

    #118217

    Purpose

    Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    xlH2B(1-114)
    

    Tags

    Mxe-GyrA intein fused to chitin binding domain

    Expression

    Bacterial

    Mutation

    Lysine 114 to alanine

    Promoter

    T7

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLJM1-FLAG-Kaptin

    Plasmid

    #100511

    Purpose

    Expresses FLAG tagged Kaptin in mammalian cells to make lentivirus

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    KPTN (KPTN Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmyc-GFP-TNRC6A-(935-984)

    Plasmid

    #42029

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa935-984

    Promoter

    CMV

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmyc-GFP-TNRC6A-(1027-1076)

    Plasmid

    #42019

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa1027-1076

    Promoter

    CMV

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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