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We narrowed to 14,066 results for: crispr grnas

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Showing: 9161 - 9180 of 14066 results

  2. TLCV2-RB1

    Plasmid
    #87836
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Insert
    sgRB1 (RB1 Human)
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Expression
    Mammalian
    Promoter
    Tight TRE promoter and U6
    Available Since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    87836-plasmid-map-sequence-id-176310

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  8. pdCas9 (GB1079)

    Plasmid
    #75399
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75399-plasmid-map-sequence-id-151817

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  9. pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

    Plasmid
    #75400
    Purpose
    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    hCas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    35S
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75400-plasmid-map-sequence-id-146044

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  14. pCasSA

    Plasmid
    #98211
    Purpose
    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9
    Depositor
    Quanjiang Ji
    Article
    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    98211-plasmid-map-sequence-id-485072

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  16. pCRCT

    Plasmid
    #60621
    Purpose
    Plasmid encoding iCas9, tracrRNA and crRNAs
    Depositor
    Huimin Zhao
    Article
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available Since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60621-plasmid-map-sequence-id-208224

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  17. pPbU6_hdhfr/yfcu_Cas9

    Plasmid
    #216423
    Purpose
    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.
    Depositor
    Ellen Bushell
    Article
    Jonsdottir et al bioRxiv 2024.04.20.590404
    Type
    Empty backbone
    Use
    CRISPR; Expression in plasmodium berghei
    Expression
    Bacterial
    Available Since
    April 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216423-plasmid-map-sequence-id-431289

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Showing: 9161 - 9180 of 14066 results