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pNIC28-DENV3-NS5FL

Plasmid

#86013

Purpose

Expression of dengue virus 3 NS5 protein

Depositor

Dahai Luo

Publication

Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

Insert

DENV3-NS5FL

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

amino acid residues 6 to 895 of DENV3 NS5

Promoter

T7

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMarC9-R6k

Plasmid

#89477

Purpose

transposon plasmid, compatible with Tn-Seq

Depositor

George Church

Publication

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pMarC9-R6k

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Syn-SomaGCaMP7

Plasmid

#158759

Purpose

Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Publication

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP7

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pST1374-N-NLS-flag-linker-Cas9-H840A

Plasmid

#51129

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Publication

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

H840A

Promoter

CMV

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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TGFB1_pLX307

Plasmid

#98377

Purpose

Lentiviral expression of TGFB1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TGFB1 (TGFB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pSAM_AraC

Plasmid

#91569

Purpose

Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

Depositor

Harry Mobley

Publication

Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

Insert

AraC/PBAD

Expression

Bacterial

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA4TO-mito-mCherry-24xGCN4_v1

Plasmid

#60913

Purpose

A fusion protein of the SunTag 24xGCN4 v1 peptide array to a mitochondrial targeting domain and mCherry

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

mito-mCherry

Expression

Mammalian

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mVenus-N1

Plasmid

#54640

Purpose

Localization: N1 Cloning Vector, Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Publication

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Tags

mVenus

Expression

Mammalian

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

8 Articles
p5E-mpeg1.1

Plasmid

#75023

Purpose

Can be used to drive expression in zebrafish microglia.

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

mpeg1.1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMX IkB alpha M

Plasmid

#12329

Depositor

Inder Verma

Publication

Van Antwerp et al Science. 1996 Nov 1. 274(5288):787-9.

Insert

IkB alpha M (Nfkbia Mouse)

Expression

Mammalian

Mutation

Dominant-negative mutant. Many signal transductio…

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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RN3P-KDM5B-FLAG

Plasmid

#86398

Purpose

FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

Depositor

Wei Xie

Publication

Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

Insert

KDM5B (Kdm5b Mouse)

Tags

3x-FLAG

Expression

Mammalian

Promoter

Lac

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)

Plasmid

#145843

Purpose

Lentiviral vector genome to ectopically express human TMPRSS2 variant 1

Depositor

Caroline Goujon

Publication

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human TMPRSS2 variant 1 (TMPRSS2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G8V

Promoter

SFFV

Available since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pINDUCER21-ERG

Plasmid

#51301

Purpose

Expression of ERG after doxycycline administration

Depositor

George Daley

Publication

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

v-ets avian erythroblastosis virus E26 oncogene homolog (ERG Human)

Use

Lentiviral

Tags

n/a

Mutation

full length ORF

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PRKAA1

Plasmid

#20595

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKAA1 (PRKAA1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pLenti-Synapsin-pHoenix-WPRE

Plasmid

#70111

Purpose

lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles

Depositor

Christian Rosenmund

Publication

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

pHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)

Use

Lentiviral

Tags

Arch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…

Promoter

Synapsin

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Green Glifon4000

Plasmid

#126208

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Publication

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon4000

Expression

Mammalian

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mKO2-SLBP(18-126)

Plasmid

#83914

Purpose

Fluorescent probe for S/G2 cell cycle transition

Depositor

Michael Lin

Publication

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mKO2-SLBP(18-126) (SLBP Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pOPINM

Plasmid

#26044

Depositor

Ray Owens

Publication

Berrow et al Nucleic Acids Res. 2007. 35(6):e45.

Insert

His-MBP-3C-lacZ

Tags

MAHHHHHHSSG_MBP_LEVLFQGP

Expression

Bacterial, Insect, and Mamm…

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRK7-HA-S6K1-E389-deltaCT

Plasmid

#8993

Depositor

John Blenis

Publication

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

T389E, stop codon at amino acid 402

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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476 p5E-mcs

Plasmid

#26029

Depositor

Nathan Lawson

Publication

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Type

Empty backbone

Use

Gateway Cloning

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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