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pXTK094 [Term_rrnB-T1_PartType7]

Plasmid

#229331

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Term_rrnB-T1_PartType7

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

rrnB-T1 Terminator

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK035 [Origin_RK2-oriT_PartType3]

Plasmid

#229272

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RK2-oriT_PartType3

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

RK2-oriT Origin

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK036 [Con-Pro_rpsM-Xantho_PartType5]

Plasmid

#229273

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_rpsM-Xantho_PartType5

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

rpsM Promoter (Xanthobacter flavus GJ10)

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK009 [Marker_TetR-tetAR_PartType4]

Plasmid

#229246

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_TetR-tetAR_PartType4

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

TetR-tetAR

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-AHR-Brd 302

Plasmid

#219238

Purpose

Transcription factor AHR with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

AHR (AHR Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBJ035_MTB_gtACR2_KGC_eGFP_ER2

Plasmid

#209278

Purpose

Ubiquitously expresses gtACR2 in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

gtACR2_KGC_eGFP_ER2

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.13_GagZip-MCP_Zip-P2A-eGFP

Plasmid

#205528

Purpose

Express HIV GagZip-MCP_Zip-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

GagZip-MCP_Zip

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

Plasmid

#182887

Purpose

Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)

Depositor

Daniel Gerlich

Article

David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

Insert

HAUS 6 (HAUS6 Human)

Use

Lentiviral

Tags

EGFP

Expression

Bacterial and Mammalian

Mutation

silet mutations in aa 197 - 203 to confer resista…

Promoter

EF1alpha core

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJH19

Plasmid

#172141

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp pylS; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166017

Purpose

mammalian expression of HCoV-NL63 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-NL63 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDY1243-Fluorescent RADARS

Plasmid

#182540

Purpose

Express Fluorescent RADARS in bicistronic vector with mCherry as control gene

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

mNeon

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mRuby3-18aa-Tubulin

Plasmid

#74256

Purpose

Mammalian expression of N-terminal fusion of mRuby3 to Tubulin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Tubulin

Tags

mRuby3

Expression

Mammalian

Promoter

CAG and CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-N-FLAG-HA-TBK1

Plasmid

#131791

Purpose

Lentiviral vector expressing WT TBK1

Depositor

Alicia Pickrell

Article

Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

Insert

TBK1 WT (TBK1 Human)

Use

Lentiviral

Tags

N-FLAG-HA

Expression

Mammalian

Mutation

WT full length

Promoter

CMV

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-BRD3

Plasmid

#65377

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD3 (BRD3 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-PDGFRA

Plasmid

#116769

Purpose

Lentiviral expression of PDGFRA

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PDGFRA (PDGFRA Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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p(A)n-PAUSE

Plasmid

#118068

Purpose

Transcription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

Plasmid

#118617

Purpose

Lentiviral expression of mTurq2 tagged PCNA

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

TK-NLS-mTurq2-PCNA (PCNA Human)

Use

Lentiviral

Tags

mTurq2

Expression

Mammalian

Promoter

hPGK

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-A3-HA-ubiquitin

Plasmid

#220145

Purpose

Expresses A3-Ub for E3 substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

Ubiquitin C (UBC Human)

Tags

A3-HA

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBLind

Plasmid

#113109

Purpose

Vector encoding the RFP gene under blue light-inducible promoter BindF1.

Depositor

Chueh Loo Poh

Article

Jayaraman et al ACS Synth Biol. 2018 Apr 20;7(4):986-994

Insert

RFP

Use

Cell-free

Expression

Bacterial

Promoter

BindF1

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pICE-FLAG-NAT10-siR-WT

Plasmid

#59365

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Larrieu et al Science. 2014 May 2;344(6183):527-32. do

Insert

human NAT10 (NAT10 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Mutations to render cDNA resistant to a siRNA

Promoter

CMV-tet

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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