We narrowed to 24,060 results for: crispr

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STAgR_Neo

Plasmid

#102992

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Promoter

U6

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pspCas9-Klenow-ST2-com-vector

Plasmid

#176235

Purpose

Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Type

Empty backbone

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ144-ZmUbi-tRNA

Plasmid

#158402

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCH68 (dAsCas12a-3xKRAB piggyBac)

Plasmid

#217334

Purpose

piggyBac expression of dAsCas12a-3xKRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

dAsCas12a

Use

CRISPR

Tags

nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

Expression

Mammalian

Mutation

E993A

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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LbCas12a-D156R

Plasmid

#213744

Purpose

For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT006

Plasmid

#182716

Purpose

Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

CasRx crRNA cloning backbone

Use

CRISPR

Tags

GFP in cloning site of crRNA for easier screening…

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-PXN

Plasmid

#227315

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of PXN (PXN Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDECKO_Malat1_A

Plasmid

#72620

Purpose

Expresses two gRNAs targeting MALAT1 promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward Malat1

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcU6_1 sgRNA

Plasmid

#92395

Purpose

Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

chick U6.1 promoter and gRNA cloning cassette

Use

CRISPR

Expression

Mammalian

Promoter

chick U6.1

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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CRISPseq-mCherry-backbone

Plasmid

#85708

Purpose

Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.

Depositor

Ido Amit

Article

Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDECKO_Malat1_C

Plasmid

#72622

Purpose

Expresses two gRNAs targeting MALAT1 promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward Malat1

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pRB14

Plasmid

#52522

Purpose

expresses a myc-tagged version of hCas9 in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

S. pyogenes cas9 with humanized codon bias

Tags

myc

Expression

Insect

Promoter

tubulin

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCR1278

Plasmid

#111121

Purpose

Expresses XRCC2_-_152373174.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

XRCC2 gRNA (XRCC2 Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1279

Plasmid

#111122

Purpose

Expresses XRCC2_+_152373143.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

XRCC2 gRNA (XRCC2 Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1280

Plasmid

#111123

Purpose

Expresses XRCC3_+_104181759.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

XRCC3 gRNA (XRCC3 Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1281

Plasmid

#111124

Purpose

Expresses XRCC3_+_104181786.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

XRCC3 gRNA (XRCC3 Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDECKO_UCA1

Plasmid

#72628

Purpose

Expresses two gRNAs targeting the UCA1 promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward UCA1

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCR1319

Plasmid

#111116

Purpose

Expresses UBE2T_+_202311076.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

UBE2T gRNA (UBE2T Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

Plasmid

#181791

Purpose

Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJZC588

Plasmid

#62315

Purpose

sgRNA with 2x MS2 (wt+f6) for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x MS2 (wt+f6) binding module

Expression

Yeast

Promoter

SNR52

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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