We narrowed to 28,450 results for: tat

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TS-PE2-C-WPRE

Plasmid

#176650

Purpose

Expresses C-terminus of PE2 in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

C-terminus of PE2
Artificial SA
WPRE
bGH poly(A)

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Promoter

None

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-S63C-GFP

Plasmid

#78911

Purpose

Mammalian expression of BAP1 with S63E and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

S63C

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pRQ-hRasV12

Plasmid

#18113

Depositor

Alex Swarbrick

Article

Swarbrick et al Proc Natl Acad Sci U S A. 2008 Apr 8. 10

Insert

h-RasV12G (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V, constitutively active

Available Since

Feb. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B-E2F2

Plasmid

#202521

Purpose

Expressing human E2F2 protein with FLAG-tag

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

E2F2 (E2F2 Human)

Tags

FLAG-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL-hygro-FAKY925F

Plasmid

#216547

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: Y925F ) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (Y925F) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation Y925F

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

Plasmid

#134331

Purpose

CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized Nme2Cas9

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-LplA(AAG)

Plasmid

#61307

Purpose

Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression

Depositor

Alice Ting

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

Insert

E. coli lipoic acid ligase

Tags

6xHis, Flag, T7 tag, and Xpress epitope

Expression

Mammalian

Mutation

E20A, F147A, H149G

Promoter

CMV

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS HSPH3

Plasmid

#19536

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 4-like (HSPA4L Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GW-mCit-PA

Plasmid

#113449

Purpose

ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

mCitrine-ProteinA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1417 (No intron)

Plasmid

#69898

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Mammalian

Mutation

F169L in ZKSCAN1

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad1 (4SP/AP+AAVA)

Plasmid

#14955

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

Insert

Smad1 (4SP/AP+AAVA) (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Four Serine to Alanine mutations in the linker re…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2b-K660E

Plasmid

#45705

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K660E

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
HDM_omicron_B11529_IDTDNA

Plasmid

#204142

Purpose

Expression plasmid for SARS-CoV-2 BA.1 variant spike

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

SARS-CoV-2 BA.1 variant spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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bArr1-ALFA

Plasmid

#201496

Purpose

Expression of the beta-arrestin1 containing a C-terminal ALFA tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr1-ALFA (ARRB1 Human)

Tags

ALFA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pB-TAG-HES7

Plasmid

#130935

Purpose

PiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

HES7 (HES7 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pH4R-P2A-mCherry-N1

Plasmid

#84333

Purpose

Co-expresses (untagged) Histamine-4 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H4R-P2A-mCherry (HRH4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS DNAJB9

Plasmid

#19559

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pGL3-p27 T187A Luc

Plasmid

#23048

Depositor

William Kaelin

Article

Zhang et al Nat Med. 2004 Jun . 10(6):643-8.

Insert

p27 (CDKN1B Human)

Tags

Luciferase

Expression

Mammalian

Mutation

T187A: mutation at codon 187 changing from T (ACG…

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-PLAA-Strep-HA

Plasmid

#113484

Purpose

Expression of human PLAA with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

PLAA (PLAA Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 IntS6 AA 1035-1284

Plasmid

#196904

Purpose

Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 6 (IntS6 Fly)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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