We narrowed to 9,895 results for: EPO

1. pSMALB-ATF4.14

   Plasmid

   #155034

   Purpose

   Fluorescent reporter for ATF4 translation, Positive Control

   Depositor

   Peter van Galen, John Dick

   Article

   van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

   Inserts

   ATF4 5' UTR (Atf4 Mouse)
   ATF4-eGFP fusion ORF
   TagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   uORF1 and uORF2 start codons mutated

   Promoter

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

   Plasmid

   #210523

   Purpose

   AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   GFP-KASH
   U6-gRNA-directcapture
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-mCherry-Tandem-PSAM4-GlyR

   Plasmid

   #208361

   Purpose

   Expression of the inhibitory chemogenetic tool PSAM4-GlyR and mCherry reporter. Application of ultrapotent agonists induces neuronal silencing.

   Depositor

   David Bennett

   Article

   Perez-Sanchez et al Sci Transl Med. 2023 Oct 4;15(716):eadh3

   Insert

   mCherry-TANDEM-PSAM4-GlyR
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-TGFb1

   Plasmid

   #101762

   Purpose

   TGFb1 promoter reporter

   Depositor

   Yuh-Shan Jou

   Article

   Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

   Insert

   transforming growth factor beta 1 promoter (TGFB1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLHCX-ATF4 mScarlet NLS

   Plasmid

   #115970

   Purpose

   ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   activating transcription factor 4 (ATF4 Synthetic, Human)
   

   Use

   Retroviral

   Tags

   HA tag, c-myc NLS, and mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. TDP-REGv1(mCherry-FLAG) in pTwist-CMV

   Plasmid

   #216165

   Purpose

   Expression of mCherry specifically in cells with TDP-43 knockdown. Uses AARS1-derived cryptic exon to control mCherry expression. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mCherry with C-terminal FLAG tag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

   Plasmid

   #220612

   Purpose

   Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS Donor mClover-LMNA

   Plasmid

   #98970

   Purpose

   Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1

   Plasmid

   #212522

   Purpose

   Encodes SARS-CoV-2 variant XBB.1 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike XBB.1 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179430

    Purpose

    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-HS4-mch (SH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-IP-mCherry-TOSI

    Plasmid

    #172496

    Purpose

    mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    PDCD4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    Mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    MLV LTR

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPGC1a_Proximal Promoter-FLuc2P

    Plasmid

    #154259

    Purpose

    Destabilized firefly luciferase reporter containing the Human PGC-1alpha Proximal Promoter from -500 to +52.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    PGC-1a Proximal Promoter (PPARGC1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGC-1alpha Proximal Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

    Plasmid

    #223965

    Purpose

    Dual fluorescent reporter for histone H2B and for Cdt1

    Depositor

    Hee Won Yang

    Article

    Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

    Insert

    H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670 and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPGC1a_Distal Promoter-FLuc2P

    Plasmid

    #154260

    Purpose

    Destabilized firefly luciferase reporter containing the upstream Human PGC-1alpha Distal Promoter from -500 to +52.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    PGC-1a Distal Promoter (PPARGC1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGC-1alpha Distal Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIS1 mLPIN1

    Plasmid

    #60803

    Purpose

    Luciferase reporter to measure miR-155 mediated differential repression. Contains LPIN1 3' UTR and mutated miR-155 sites

    Depositor

    David Bartel

    Article

    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

    Insert

    LPIN1 3'UTR and mutated miR-155 binding site (LPIN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutated miR-155 binding site

    Promoter

    thymidine kinase (HSV-TK promoter)

    Available Since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSET-iSeroSnFR

    Plasmid

    #128483

    Purpose

    Fluorescent reporter for serotonin (bacterial expression)

    Depositor

    Lin Tian

    Article

    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

    Insert

    iSeroSnFR
    

    Use

    Tags

    His, T7, and cpsfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-RKIP_RFP

    Plasmid

    #199215

    Purpose

    RKIP (PEBP1) expression plasmid with a RFP reporter on the backbone.

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Inserts

    RKIP (PEBP1 Human)
    RFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and PGK

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1.16

    Plasmid

    #212532

    Purpose

    Encodes SARS-CoV-2 variant XBB.1.16 Spike for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike XBB.1.16 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TDP-REGv2 Cryptic PE-Max

    Plasmid

    #216163

    Purpose

    Expression of prime editor PEMax (Plasmid #174820) only in cells with TDP-43 loss of function. Note: It is not recommended to produce lentivirus containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    PEMax for prime editing with internal cryptic exon
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSBbi-NLSmCherryNLS Clover-HDHB

    Plasmid

    #190223

    Purpose

    Fluorescent cell cycle reporter

    Depositor

    Laura Heiser

    Article

    Gross et al Nat Commun. 2023 Jun 10;14(1):3450. doi:

    Inserts

    Human DNA Helicase B (amino acids 994-1087) (HELB Human)
    NLS-mCherry-NLS-P2A-Puromycin
    

    Use

    Transposon

    Tags

    Clover fluorescent protein

    Expression

    Mammalian

    Mutation

    Amino Acids 994-1087

    Promoter

    EF1a and RPBSA

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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