Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 10,007 results for: Gnas

Clear keywords
Showing: 9281 - 9300 of 10007 results

  1. CCR3-DuET

    Plasmid
    #213199
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR3 (CCR3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213199-plasmid-map-sequence-id-430762

    Enlarge

  2. DRD1-DuET

    Plasmid
    #213227
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    DRD1 (DRD1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213227-plasmid-map-sequence-id-431800

    Enlarge

  3. GPR123-DuET

    Plasmid
    #213262
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR123 (ADGRA1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213262-plasmid-map-sequence-id-430812

    Enlarge

  5. GPR146-DuET

    Plasmid
    #213265
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR146 (GPR146 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213265-plasmid-map-sequence-id-430903

    Enlarge

  7. pCDF-Az

    Plasmid
    #197101
    Purpose
    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine
    Depositor
    Kensaku Sakamoto
    Article
    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20
    Insert
    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    Expression
    Bacterial
    Mutation
    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…
    Available Since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197101-plasmid-map-sequence-id-390718

    Enlarge

  8. CALCRL-DuET

    Plasmid
    #213194
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CALCRL (CALCRL Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    213194-plasmid-map-sequence-id-431751

    Enlarge

  9. CHRM3-DuET

    Plasmid
    #213209
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CHRM3 (CHRM3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213209-plasmid-map-sequence-id-430771

    Enlarge

  11. dCas9-NLS-gs10-cERb447

    Plasmid
    #188257
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cERb447 (ESR1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188257-plasmid-map-sequence-id-374591

    Enlarge

  12. Cer(1-158)-RGS7

    Plasmid
    #55760
    Purpose
    An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    mCer(1-158)-RGS7 (RGS7 Human, Aequorea victoria)
    Tags
    Cer(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    RGS7 was amplified via PCR, which added an N-term…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55760-plasmid-map-sequence-id-115314

    Enlarge

  14. BDKBR1-DuET

    Plasmid
    #213189
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    BDKBR1 (BDKRB1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213189-plasmid-map-sequence-id-430754

    Enlarge

  15. HTR6-DuET

    Plasmid
    #213326
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR6 (HTR6 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213326-plasmid-map-sequence-id-431819

    Enlarge

  16. RH4-bio

    Plasmid
    #47779
    Purpose
    Expresses enzymatically monobiotinylated partial RH4 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RH4 (partial)
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47779-plasmid-map-sequence-id-68798

    Enlarge

  18. EBA181-bio

    Plasmid
    #47744
    Purpose
    Expresses enzymatically monobiotinylated full-length EBA181 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBA181
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47744-plasmid-map-sequence-id-68752

    Enlarge

  20. nCre-ST-NLS-P2A-NLS-pSC-cCre

    Plasmid
    #207638
    Purpose
    A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available Since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207638-plasmid-map-sequence-id-409880

    Enlarge

Showing: 9281 - 9300 of 10007 results