159,258 results

1. FTO-dCas9

   Plasmid

   #134781

   Purpose

   Expression fusion protein FTO-dCas9 in Mammalian cells

   Depositor

   Shu-Bing Qian

   Article

   Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

   Insert

   FTO and inactive dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. GAL-Aur1-2GFP-Hygromycin

   Plasmid

   #184775

   Purpose

   Yeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   proGAL-Aur1 (AUR1 Budding Yeast)
   

   Use

   Tags

   2EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Gamillus/ pcDNA3

   Plasmid

   #124837

   Purpose

   mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

   Insert

   Gamillus
   

   Use

   Tags

   No tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS426GFP-2×PH(PLCδ)

   Plasmid

   #36092

   Depositor

   Scott Emr

   Article

   Stefan et al Mol Biol Cell. 2002 Feb;13(2):542-57.

   Insert

   2xPH(PLCdelta) fusion
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   contains aa11-140 of PLCδ (including the PH domai…

   Promoter

   PRC1

   Available since

   June 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

   Plasmid

   #100848

   Purpose

   AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1 and AAV9

   Insert

   jRCaMP1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV PIG (Puro IRES GFP)

   Plasmid

   #18751

   Depositor

   Scott Lowe

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTagRFP-C1-Fibrillarin

   Plasmid

   #70649

   Purpose

   Expresses Fibrillarin nucleolar marker with TurboRFP reporter.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   Fibrillarin (FBL Human)
   

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pET22b_PstS_1

   Plasmid

   #78198

   Purpose

   Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A197C (please see depositors comment below)

   Promoter

   T7

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV_IRES_GFP_CD16a

   Plasmid

   #196189

   Purpose

   Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

   Depositor

   Ana Ruiz-Saenz

   Article

   Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

   Insert

   CD16a (FCGR3A Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Phenylalanine 158 to Valine

   Promoter

   LTR

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 AIF 1-90 mCherry FRB

    Plasmid

    #67530

    Purpose

    For expression of mCherry fused FRB to the mitochondrial innermembrane

    Depositor

    Stephen Tait

    Article

    Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

    Inserts

    AIF a.a. 1 - 90
    mCherry
    FRB
    

    Use

    Tags

    AIF 1 - 90, FRB, and mCherry

    Expression

    Mammalian

    Mutation

    T->L

    Promoter

    CMV

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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