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pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

Plasmid

#177320

Purpose

Lentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).

Depositor

Steven Chu, Xiaolin Nan

Article

Nan et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

Insert

KRAS 4B (KRAS Human)

Use

Lentiviral

Tags

FKBP-derived dimerization domain (DD, or DmrB) an…

Expression

Mammalian

Mutation

G12D

Promoter

TetOn-CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJL1-eBFP2

Plasmid

#102640

Purpose

In vitro expression of eBFP2 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

eBFP2

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

Plasmid

#162877

Purpose

Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)

Tags

mEos2

Expression

Mammalian

Mutation

Insert consists of AA1-170

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pYJA5

Plasmid

#217778

Purpose

The empty vector for quadruple sgRNA cloning

Depositor

Adriano Aguzzi

Article

Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Puromycin resistance gene, T2A, and TagBFP

Expression

Mammalian

Promoter

PGK, CMV, U6

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX-307_LgBiT

Plasmid

#191211

Purpose

Lentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

LgBiT (large bit)

Use

Lentiviral and Luciferase

Mutation

AB030246.1 (mutated in PMID 26569370)

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHLsec-FAP

Plasmid

#239817

Purpose

Expresses human fibroblast activation protein alpha (FAP) in mammalian cells

Depositor

Ingrid De Meester

Article

Verhulst et al Eur J Med Chem. 2026 Feb 15;304:118494.

Insert

Fibroblast activation protein alpha (FAP Human)

Tags

8xHIS tag and TwinStrep tag

Expression

Mammalian

Mutation

No mutations

Promoter

Chicken beta-actin

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pJL1-dTomato

Plasmid

#102631

Purpose

In vitro expression of tdTomato from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

tdTomato

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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sgKRAS(A)

Plasmid

#85566

Purpose

Expresses sgRNA targeting human KRAS around codon G12

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

KRAS proto-oncogene, GTPase (KRAS Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

Plasmid

#176851

Purpose

lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hYAP1-5SA (YAP1 Human)

Use

Lentiviral

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ASAP4b-Kv-WPRE

Plasmid

#201030

Purpose

Expresses somatically enriched ASAP4b in neurons, can be used to package AAV

Depositor

Michael Lin

Article

Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

Insert

ASAP4b-Kv

Use

AAV

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn CaBLAM

Plasmid

#244227

Purpose

Bioluminescent reporter for calcium signaling in neurons

Depositor

Ute Hochgeschwender

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Inserts

mNeonGreen
CaBLAM

Use

AAV

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ASAP4e-Kv-WPRE

Plasmid

#201031

Purpose

Expresses somatically enriched ASAP4e in neurons, can be used to package AAV

Depositor

Michael Lin

Article

Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

Insert

ASAP4e-Kv

Use

AAV

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-ASC1

Plasmid

#75134

Purpose

Expresses N-terminal Flag-tagged ASC1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

PYD and CARD domain containing (Pycard Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

F23S (please see depositor comments below)

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 ORF3a C-V5-tag

Plasmid

#179980

Purpose

Mammalian expression vector for SARS-CoV-2 ORF3a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-2 ORF3a (ORF3a Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET-iSeroSnFR

Plasmid

#128483

Purpose

Fluorescent reporter for serotonin (bacterial expression)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Tags

His, T7, and cpsfGFP

Expression

Bacterial

Promoter

T7

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJL1-mOrange

Plasmid

#102632

Purpose

In vitro expression of mOrange from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mOrange

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1.sh.beta-catenin.1248

Plasmid

#19761

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against beta-catenin (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)

Plasmid

#162880

Purpose

Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3 711-1034

Plasmid

#75141

Purpose

Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 711-1034

Promoter

CMV

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3 1-90

Plasmid

#75137

Purpose

Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 1-90

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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