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pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Expression

Mammalian

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti TurboRFP BlastR

Plasmid

#102343

Purpose

Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

TurboRFP

Use

Lentiviral

Tags

N/A

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD_FLAG-GFP(Y39TAG)-6His

Plasmid

#223510

Purpose

Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

Depositor

Edward Lemke

Article

Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

Insert

GFP(Y39TAG)

Tags

6xHis and FLAG

Expression

Bacterial

Mutation

Y39TAG

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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dCas13d-dsRBD-APEX2

Plasmid

#154939

Purpose

TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

Tags

V5

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Stargazin-mEGFP-iLID

Plasmid

#178523

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_EV

Plasmid

#183116

Purpose

Empty vector control for pLenti_EF1a constructs

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

Small multiple cloning site

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR TDP43 WT YFP

Plasmid

#27470

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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#1.4 post-G-SynapShot-mScarlet

Plasmid

#214716

Purpose

Expresses green #1.4 post-SynapShot and mScarlet

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#1.4 post-G-SynapShot

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mTagBFP2-WPRE

Plasmid

#193339

Purpose

AAV-mediated expression of mTagBFP2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mTagBFP2

Use

AAV

Expression

Mammalian

Mutation

A XhoI site in the coding sequence is silenced

Promoter

TRE

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SspB-mScarlet-I

Plasmid

#178521

Purpose

Expresses SspB-mScarlet-I in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

SspB-mScarlet-I

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMH-SFB

Plasmid

#99391

Purpose

Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBIG2ab

Plasmid

#80616

Purpose

pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-mHRI-FLAG-IP

Plasmid

#101791

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse HRI

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Hri (Eif2ak1 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pWN_U6-B2M-miniGag

Plasmid

#228957

Purpose

miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L

Plasmid

#27000

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti EF1a GFP-mCherry-mPlin1

Plasmid

#189005

Purpose

Fluorescent reportor for lipophagy

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

GFP, mChery, Plin1 (Plin1 Mouse)

Use

Lentiviral

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Lyn-FRB-GFP11

Plasmid

#181863

Purpose

Plasma membrane-localized GFP11; for plasma membrane targeting of FluoSTEPs.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

Lyn-FRB-GFP11

Tags

GFP11 and N-terminal targeting sequence from Lyn …

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-ROCK1

Plasmid

#187296

Purpose

Express GFP ROCK1

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (a.k.a. 1110055K06Rik, Rock-I) (Rock1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-CRY2-mCherry

Plasmid

#58368

Purpose

Mammalian codon optimized CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Insert

CRY2[1-498] (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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#2.4 pre-G-SynapShot-TagBFP

Plasmid

#214721

Purpose

Expresses green #2.4 pre-SynapShot and TagBFP

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#2.4 pre-G-SynapShot

Use

AAV

Tags

TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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