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pMXs-hs-CDC34

Plasmid

#52731

Purpose

retroviral expression of human CDC34

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

CDC34 (CDC34 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RasAR-NES

Plasmid

#205567

Purpose

Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

RasAR-NES (RAF1 Human, Synthetic)

Tags

Nuclear export signal (NES)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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AICSDP-166:CDH1-mEGFP

Plasmid

#193921

Purpose

Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1

Depositor

Allen Institute for Cell Science

Insert

cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)

Use

AAV and CRISPR; Donor template

Tags

linker-mEGFP

Mutation

homology arms contain point mutations to disrupt …

Available Since

Dec. 22, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-PGK-EGFP-ALDH1A1-Neo

Plasmid

#189742

Purpose

The construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A1 (ALDH1A1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mScarlet-BlastR [M1G]

Plasmid

#171814

Purpose

Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mScarlet-T2A-BlastR

Use

Gene tagging

Mutation

BlastR: Changed Methionin 1 to Glycine.

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839)

Plasmid

#163509

Purpose

AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq applications.

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Has Service

AAV PHP.eB

Insert

H2B-SYFP2

Use

AAV

Promoter

hSyn1

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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C1-MPAct(delta1-6)-mRuby3

Plasmid

#155223

Purpose

F-actin bidning mutant of MPAct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Mutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b

Tags

mRuby3

Expression

Mammalian

Mutation

Deletion of first 6 AA from F-tractin (9-14 from …

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT

Plasmid

#132667

Purpose

Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-ODD-T2A-TdT (DNTT Human, Streptococcus pyogenes)

Expression

Mammalian

Promoter

4xHRE_YB TATA

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) PHLDB1

Plasmid

#107503

Purpose

Expresses PHLDB1, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

PHLDB1 (PHLDB1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Nucleotide changes 1236 T>C, 1365 C>T, 1371…

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA

Plasmid

#104097

Purpose

Expresses TREAT-PK co-localization control in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Tags

24xMS2 stem loops in 3'UTR and 24xPP7 stem l…

Expression

Mammalian

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARMA2sh-FLAG

Plasmid

#97071

Purpose

Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-TRAF4

Plasmid

#58318

Purpose

EGFP-tagged mouse TRAF4

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xHA-MKK4-EE

Plasmid

#47577

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK4-EE (MAP2K4 Human)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Lacks the first 36 residues of the RefSeq protein…

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pMXs-IP-EGFP-ULK1(1-828)

Plasmid

#38198

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 829-1051

Available Since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K5S

Plasmid

#34598

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Fragment 1781-2086 of KLF4

Promoter

CMV

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
-962 human cyclin D1 promoter EtsA site mutant pGL3Basic

Plasmid

#32729

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Luciferase reporter gene

Mutation

-822 EtsA site AGGAA deletion

Promoter

-962 CCND1 promoter

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pIRESneo-MPG(N169D)

Plasmid

#23259

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

Insert

N-methylpurine DNA glycosylase (N169D) (MPG Human)

Expression

Mammalian

Mutation

N169D

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
pRSET FLIPglu-600uDelta11 Aphrodite

Plasmid

#13569

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta11 Aphrodite

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-CD79B-RFP

Plasmid

#187005

Purpose

Expression of human CD79B as RFP fusion protein

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

CD79B (CD79B Human)

Use

Lentiviral

Tags

tagRFP

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-H2B-GFP

Plasmid

#184777

Purpose

Expresses H2B-GFP in human pluripotent stem cells

Depositor

Ivana Barbaric

Article

Price et al Curr Protoc. 2022 May;2(5):e435. doi: 10

Insert

H2BC11 (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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