Addgene

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px458_2A_GFP_sgRNA_PELO

Plasmid

#127124

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA PELO (PELO Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Ub-R-GFP

Plasmid

#11939

Depositor

Nico Dantuma

Article

Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

Insert

Ub-R-GFP

Use

Tags

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Promoter

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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1B-GFP1-10

Plasmid

#193867

Purpose

E. coli expression vector for Hisx6 tagged GFP1-10.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

sfGFP1-10

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

Plasmid

#98927

Purpose

Cre dependent AAV expression of GFP spaghetti monster myc construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has Service

AAV1

Insert

GFP-smFP-myc

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMax_GFP_C289T

Plasmid

#177826

Purpose

Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

Depositor

Leona Samson, Zachary Nagel

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

GFP_C289T

Use

Tags

Expression

Mammalian

Mutation

C289T

Promoter

CMV

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_KIAA0100_PITPNC1

Plasmid

#205837

Purpose

Express mEGFP-tagged fusion protein, KIAA0100_PITPNC1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

KIAA0100_PITPNC1 (KIAA0100, PITPNC1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP_TRPM8

Plasmid

#64879

Purpose

Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

Depositor

Sebastián Brauchi

Article

Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

Insert

Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)

Use

Tags

eGFP

Expression

Mutation

Promoter

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_BCR_ABL1

Plasmid

#205780

Purpose

Express mEGFP-tagged fusion protein, BCR_ABL1_p210 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

BCR_ABL1 (BCR, ABL1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_KIAA1549_BRAF

Plasmid

#205838

Purpose

Express mEGFP-tagged fusion protein, KIAA1549_BRAF from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

KIAA1549_BRAF (KIAA1549, BRAF Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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3xmEGFP

Plasmid

#127800

Purpose

Homo-trimer mEGFP-mEGFP-mEGFP expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mEGFP-mEGFP-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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Nup58_mEGFP(-7)

Plasmid

#163424

Purpose

Mammalian expression of Nup58 with a mEGFP (missing the first 7 amino acids) at amino acid position 412

Depositor

Sanford Simon

Insert

Nup58 (NUPL1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBbA6c_PsulA_sfGFP

Plasmid

#224587

Purpose

SOS response reporter

Depositor

Ophelia Venturelli

Article

Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PsulA

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pNFkB:eGFP

Plasmid

#44922

Depositor

John Rawls

Article

Kanther et al Gastroenterology. 2011 Jul;141(1):197-20

Insert

EGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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SFG.wtCNb_opt.IRES.eGFP

Plasmid

#22492

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Sep 21. ():.

Insert

Codon optimised wild type Calcineurin B (PPP3R1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_MYC_MYC

Plasmid

#205862

Purpose

Express mEGFP-tagged fusion protein, MYC_MYC from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

MYC_MYC (MYC Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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3XHalo_GFP_TRF1#1

Plasmid

#164644

Purpose

tag TRF1 with GFP and Halo

Depositor

Huaiying Zhang, Michael Lampson

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

TRF1 (TERF1 Human)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDDGFP_LEU2D

Plasmid

#58352

Purpose

Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

Depositor

Simon Newstead

Article

Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

Type

Empty backbone

Use

Tags

GFP-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAKgfp1

Plasmid

#14076

Depositor

Attila Karsi

Article

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Green fluorescent protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2007

Availability

Academic Institutions and Nonprofits only
CL20_mEGFP_MTF1_SF3A3

Plasmid

#205861

Purpose

Express mEGFP-tagged fusion protein, MTF1_SF3A3 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

MTF1_SF3A3 (MTF1, SF3A3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pColE1_70a_deGFP_AmpR

Plasmid

#119161

Purpose

Expresses deGFP from a strong sigma 70 promoter

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

deGFP

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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