-
Plasmid#135448PurposeHuman expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.DepositorInsertdCas9-BFP-KRAB
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
scFv-sfGFP-DNMT3A1
Plasmid#102278PurposeThe plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1DepositorInsertDNMT3A1 (DNMT3A Human)
UseLentiviralTagsscFV-sfGFPExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10704
Plasmid#183957PurposeU6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFPDepositorInsertSKSKSO array
UseLentiviralTagsEF1a-Puro-T2A-TagBFPExpressionMammalianMutationPromoterU6Available sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag
Plasmid#199457PurposeExpression of dCas13-VPR in mammalian cellsDepositorInsertdCas13
UseLentiviralTagsVPR-T2A-HaloTagExpressionMammalianMutationNuclease-dead H133A and H1058APromoterCAGAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
LiPOP1-NanoLuc
Plasmid#168265PurposeChemo-optogenetic control of necroptotic cell death by coupling LiPOP1 with NanoLuc-aided bioluminescence optogenetic stimulation (NanoLOGS)DepositorInsertMLKL(1-125) (MLKL Human)
UseTagsmCherry, NanoLuc, CRY2-PHRExpressionMammalianMutationH15A, K16A, R17APromoterCMVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hTRF2
Plasmid#103803PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
crTet in pSLQ8453
Plasmid#183965PurposeU6-crTet-EF1a-Puro-T2A-BFP-WPREDepositorInsertcrTet
UseLentiviralTagsEF1a-Puro-T2A-BFPExpressionMammalianMutationPromoterU6Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hTRF2
Plasmid#103804PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry GRK2 ct in pcDNA3.1
Plasmid#64212PurposemCherry fused between Cry2 and GRK2ct to study cell migrationDepositorUseTagsmcherryExpressionMammalianMutationPromoterCMV and cmvAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry GRK3 ct in pcDNA3.1
Plasmid#64213PurposemCherry fused between Cry2 and GRK3 ct to study cell migrationDepositorUseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-CIBN
Plasmid#183937PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposureDepositorInserttdPCP-CIBN (CIB1 Synthetic, Mustard Weed)
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4
Plasmid#123658PurposeDD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromotermU6Available sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
double_sgRNA targeting e3 and e7
Plasmid#190689PurposesgRNAs targeting enhancer 3 and 7 of MYC separatelyDepositorInsertsgRNAs targeting enhancer 3 and 7 of MYC separately
UseCRISPR, Lentiviral, and Synthetic BiologyTagsBFP and PuromycinExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hNCL
Plasmid#103805PurposeBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationhNCL: A395G (K132R), A421G (K141E), A424G (K142E)…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-LacI
Plasmid#103814PurposeBLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hNCL
Plasmid#103806PurposeBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationhNCL: A395G (K132R), A421G (K141E), A424G (K142E)…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
CA-0248 EF1a_mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry_Gen2
Plasmid#231208PurposeSingle transcript cageIL-2 (mRens) + ER-renin (H2M) (Lenti)DepositorInsertmRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry (IL2 Human)
UseLentiviralTagsExpressionMutationL80F, R81D, L85V, I86V, I92FPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2
Plasmid#231207PurposeSingle transcript cageIL-10 (mRens) + ER-renin (H2M) (Lenti)DepositorInsertPuro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
UseLentiviralTagsExpressionMutationL147I, R148H, I203V, L290VPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
aTY178_pUAS_Dkk1-IRES-tagBFP2_WPRE
Plasmid#225353PurposeLentiviral vector for DKK1 and BFP expressionDepositorInsertDkk1 (Dkk1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKM 1995
Plasmid#217826PurposeExpresses 1554 bp fragment of HY4 gene in A. thaliana. This HY4 fragment shows high homology to the phr genes in prokaryotes.DepositorInsertHY4 (CRY1 Mustard Weed)
UseTagsMaltose-binding proteinExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only