We narrowed to 8,980 results for: sgRNA

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pLENTICRISPR-PABPC1-sgRNA2

Plasmid

#165942

Purpose

CRISPR-mediated gene perturbation (knock-out) of PABPC1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

PABPC1 (PABPC1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-PGAM1-sgRNA1

Plasmid

#165943

Purpose

CRISPR-mediated gene perturbation (knock-out) of PGAM1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

PGAM1 (PGAM1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-PGAM1-sgRNA2

Plasmid

#165944

Purpose

CRISPR-mediated gene perturbation (knock-out) of PGAM1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

PGAM1 (PGAM1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-SNRNP70-sgRNA1

Plasmid

#165945

Purpose

CRISPR-mediated gene perturbation (knock-out) of SNRNP70

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

SNRNP70 (SNRNP70 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-SNRNP70-sgRNA2

Plasmid

#165946

Purpose

CRISPR-mediated gene perturbation (knock-out) of SNRNP70

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

SNRNP70 (SNRNP70 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-SNRPA-sgRNA1

Plasmid

#165947

Purpose

CRISPR-mediated gene perturbation (knock-out) of SNRPA

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

SNRPA (SNRPA Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-SNRPA-sgRNA2

Plasmid

#165948

Purpose

CRISPR-mediated gene perturbation (knock-out) of SNRPA

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

SNRPA (SNRPA Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-TPI1-sgRNA1

Plasmid

#165949

Purpose

CRISPR-mediated gene perturbation (knock-out) of TPI1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-TPI1-sgRNA2

Plasmid

#165950

Purpose

CRISPR-mediated gene perturbation (knock-out) of TPI1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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tet-pLKO-sgRNA-puro

Plasmid

#104321

Purpose

3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

Depositor

Nathanael Gray

Article

Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1/TO

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgRNA

Plasmid

#124844

Purpose

Vector for Cre-dependent expression of SaCas9

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SaCas9, gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS and NLS-3xHA

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRNA-CMV-GFP

Plasmid

#85451

Purpose

Express sgRNA in mammalian cells

Depositor

Hetian Lei

Article

Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

Insert

pCMV-EGFP

Use

AAV

Promoter

pCMV-EGFP

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV enAsCas12f-HKRA-sgRNA_v7

Plasmid

#208357

Purpose

Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

Depositor

Atsushi Hoshino

Article

Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

Insert

enAsCas12f-HKRA

Expression

Mammalian

Mutation

I123H/D195K/D208R/V232A

Promoter

CMV

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pPUR-mU6-sgRNA-2XPP7

Plasmid

#194504

Purpose

Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

Depositor

Chunqing Song

Article

Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMS79 (eft-3p::Cas9 + sgRNA)

Plasmid

#154839

Purpose

Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

sgRNA

Use

CRISPR

Expression

Worm

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK-puromycin

Plasmid

#51133

Purpose

expresses sgRNA from the U6 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV enAsCas12f-YHAM-sgRNA_v7

Plasmid

#208356

Purpose

Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

Depositor

Atsushi Hoshino

Article

Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

Insert

enAsCas12f-YHAM

Expression

Mammalian

Mutation

F48Y/S188H/V232A/E316M

Promoter

CMV

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

Plasmid

#102796

Purpose

Retrovirus for delivery of one sgRNA (empty, bbsI)

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

U6 sgRNA

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sasgRNACMV- mCherry-WPREpA

Plasmid

#217015

Purpose

S. aureus sgRNAs backbone along with mCherry expression

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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