20,361 results
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Plasmid#117006PurposeExpresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pME-MLS-KillerRed
Plasmid#115517PurposeMiddle entry plasmid containing membrane bound KillerRed fluorophoreDepositorInsertMLS-KillerRed
UseGateway CloningTagsMLSAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-14-3-3 epsilon
Plasmid#116886PurposeExpresses HA-tagged human 14-3-3 epsilon in mammalian cellsDepositorAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-fDIO mCherry
Plasmid#114471PurposeFlp-dependent mCherry ControlDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV RetrogradeInsertmCherry
UseAAVPromoterEf1aAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMx-puro-MGT
Plasmid#111809PurposeRetroviral expression vector for direct cardiac reprogrammingDepositorUseRetroviralExpressionMammalianPromoterviral LTRAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSEMS-Tom20-Halo7Tag
Plasmid#111135PurposeTom20 with selflabeling tag, e.g. for fluorescence microscopyDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertReceptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)
TagsHalo7 TagExpressionMammalianPromoterCMVAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn1-tTA
Plasmid#104109PurposeAAV-mediated expression of tTA under the Syn1 promoter.DepositorInserttTA2
UseAAVPromoterhSyn1Available sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM446 (Flag-DAS+4))
Plasmid#108321PurposeOrbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.DepositorInsertattB-Flag-DAS+4
UseOrbit integrating plasmidAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVET-HA-K-RasG12V-IRES-GFP
Plasmid#107140PurposeA bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFPDepositorInsertHA-tagged Kras4B(G12V) and GFP (KRAS Human)
UseLentiviralExpressionMammalianPromoterhEF-1alphaAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCVpuro-TagRFP657
Plasmid#96939PurposeRetroviral plasmid containing TagRFP657 for labeling of cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTag Red Fluorescent Protein 657
ExpressionMammalianAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.SF-iGluSnFR.A184S
Plasmid#106198PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVMutationGltI: A184SPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHIV-H2B-eGFP
Plasmid#91776PurposeExpresses H2B protein tagged with eGFPDepositorInsertHuman Histone H2B / eGFP (H2BC11 Human)
UseLentiviralTagseGFPExpressionMammalianPromoterEF1aAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB1-mGFPmut2
Plasmid#103980PurposePlasmid encoding mGFPmut2DepositorInsertmGFPmut2
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S65A, V68L, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMXs-mGCN2-FLAG-IP
Plasmid#101794PurposeRetroviral vector encoding C-terminally FLAG tagged mouse GCN2DepositorAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FRB-mCherry-FTH1
Plasmid#100749PurposePart of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertferritin heavy chain 1 (FTH1 Human)
TagsFRB and mCherryExpressionMammalianPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330a dCas9-KRAB
Plasmid#92361PurposeModified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-KRAB
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV pCAG-FLEX-mScarlet-WPRE
Plasmid#99280PurposeCan be used to generate AAV virus that will express mScarlet in the presence of CreDepositorInsertmScarlet
UseAAV and Cre/LoxAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEThT-RvLEAM
Plasmid#90033PurposeTo express His-tagged RvLEAM in bacteriaDepositorInsertRvLEAM
TagsHisExpressionBacterialPromoterT7Available sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
4xNLS-pMJ915v2-sfGFP
Plasmid#88921PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRExpressionBacterialAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by