We narrowed to 15,130 results for: NTS

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416GPD-FUS-YFP

Plasmid

#29594

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
psiCHECK2-p27Kip1 3'UTR

Plasmid

#29476

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

p27 Kip1 3'UTR (CDKN1B Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pAAV.GFAP.SF-iGluSnFR.S72A

Plasmid

#106194

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

GFAP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCK668

Plasmid

#192637

Purpose

Expresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Mutation

SoxS has R93A and S101A mutations and dCas9-NG ha…

Promoter

BBa_J23107 and Sp.pCas9

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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FusionRed-CD36-C-10

Plasmid

#56104

Purpose

Localization: Membrane, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

CD36 (CD36 Human)

Tags

FusionRed

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)

Plasmid

#160571

Purpose

Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNAscaffold 2.1 scRNA

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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3019_pETcon-SARS-CoV-2-RBD_E484K

Plasmid

#184404

Purpose

yeast surface display of the SARS-CoV-2 Eta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

E484K

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS

Plasmid

#158628

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRS-mTagRFP-TUBA1B

Plasmid

#62850

Purpose

Donor vector for gene editing at human TUBA1B locus

Depositor

Gaudenz Danuser

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

mTagRFP-TUBA1B (TUBA1B Synthetic, Human)

Use

Yeast-e.coli shuttle vector

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pmKikGR Actin

Plasmid

#105315

Purpose

Photoconvertable cytoskeletal protein actin to study kinetics or dynamics

Depositor

Kenneth Yamada

Article

Doyle et al J Cell Sci. 2012 May 1;125(Pt 9):2244-56

Insert

actin beta (ACTB Human)

Tags

KikGR

Expression

Mammalian

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.429

Plasmid

#169461

Purpose

Encodes SARS-CoV-2 B.1.429 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.1.429 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.1.298

Plasmid

#169460

Purpose

Encodes SARS-CoV-2 B.1.1.298 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.1.298 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)

Plasmid

#160622

Purpose

TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLL

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35s_MS2:EDLL_Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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GST-TEV-FUS-267-526aa

Plasmid

#29630

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-267-526aa (FUS Human)

Tags

GST and TEV

Expression

Bacterial

Mutation

Contains FUS aa# 267-526

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
GST-TEV-FUS-1-373aa

Plasmid

#29631

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Tags

GST and TEV

Expression

Bacterial

Mutation

Contains FUS aa# 1-373

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
423Gal-FUS-YFP

Plasmid

#29615

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_mGFP-FGFR3-G380R

Plasmid

#191755

Purpose

Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Tags

mGFP

Expression

Mammalian

Mutation

c.1138G>A (G380R) + c.1131C>T (silent mutat…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-His8-aCat(660-906)

Plasmid

#203333

Purpose

Bacterial expression of alpha-catenin F-actin binding domain

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

Insert

CTNNA1 (CTNNA1 Human)

Tags

8xHis with precision protease cleavage site

Expression

Bacterial

Mutation

aa 660-906 only

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Paragranulin+linker 1

Plasmid

#176915

Purpose

express Paragranulin with linker 1 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Paragranulin + linker 1 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTE4999

Plasmid

#107555

Purpose

Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hFnCpf1(dead1)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

D917A

Promoter

CMV

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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