19,831 results

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pCE059-SiT-Cas12a-[Activ]

Plasmid

#128136

Purpose

Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Activ]-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EOS-C(3+)-EiP

Plasmid

#21313

Purpose

Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Lentiviral

Expression

Mammalian

Promoter

EOS-C(3+) promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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mCh-GluA1-CIB

Plasmid

#89444

Purpose

Allows conditional recruitment of AMPA receptors to the postsynaptic membrane

Depositor

Matthew Kennedy

Article

Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

Insert

mCh-GluA1-CIB

Expression

Mammalian

Promoter

CaMKII

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMZ374

Plasmid

#59896

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Promoter

adh1 and rrk1

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_neomycin

Plasmid

#65306

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Tags

KDEL motif and Signal Sequence

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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CFP-CD82

Plasmid

#1818

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

CD82 (CD82 Human)

Tags

CFP

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pN3-Sp1FL

Plasmid

#24543

Depositor

Guntram Suske

Insert

Sp1FL (SP1 Human)

Expression

Mammalian

Available Since

June 16, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3-FLAG-human NPM1 wt

Plasmid

#160692

Purpose

Mammalian expression of FLAG-tagged NPM1

Depositor

Charles Sherr

Article

Bertwistle et al Mol Cell Biol. 2004 Feb . 24(3):985-96.

Insert

NPM1 (NPM1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Flag Rab12 wild type

Plasmid

#86787

Purpose

mammalian expression of Rab12

Depositor

Peter McPherson

Article

Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

Insert

Rab12 (RAB12 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-4031NES

Plasmid

#105241

Purpose

A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.

Depositor

Michiyuki Matsuda

Article

Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

Insert

AMPK-EV

Expression

Mammalian

Promoter

CAGGS

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag Runx1 FL (P#1790)

Plasmid

#14585

Depositor

Matija Peterlin

Article

Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

Insert

Runx1 (Runx1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pLV-EBFP2-nuc

Plasmid

#36085

Depositor

Pantelis Tsoulfas

Type

Empty backbone

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pET28-SaCas9

Plasmid

#178901

Purpose

SaCas9 (Cas9 from Staphylococcus aureus)

Depositor

Hendrik Milting

Article

Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

Insert

SaCas9

Tags

His6-Tag, bipartite nuclear localization signal f…

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ420 - Peft-3::GFP::H2B (ubiquitous)

Plasmid

#34877

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

Peft-3 GFP H2B tbb-2 UTR

Use

Worm targeting

Mutation

S65C

Promoter

eef-1A.1 (eft-3)

Available Since

Feb. 15, 2012

Availability

Academic Institutions and Nonprofits only

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3xmNeonGreen_UtrCH

Plasmid

#129607

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Utrophin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLGB30

Plasmid

#126620

Purpose

Suicide vector for allelic replacement in Bacteroides, tetracycline selection and rhamnose-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166015

Purpose

mammalian expression of HCoV-OC43 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-OC43 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRS3 (CK2alpha, CK2beta)

Plasmid

#27092

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha (CSNK2A1 Human)

Use

Tetracycline-regulated expression

Tags

HA and Myc

Expression

Mammalian

Promoter

bidirectional tet-responsive promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLv-CMV-MyoD-ER(T)

Plasmid

#26809

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

MyoD-ER(T) (Myod1 Mouse)

Use

Lentiviral

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only
ATGL*-V5-APEX2

Plasmid

#170574

Purpose

A C-terminal APEX2 fusion to the human ATGL S47A protein

Depositor

James Olzmann

Article

Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

Insert

ATGL* (PNPLA2 Human)

Use

Lentiviral

Tags

V5-APEX2

Expression

Mammalian

Mutation

changed serine 47 to alanine

Promoter

CMV

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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