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POC1355

Plasmid

#221572

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1343, POC1344, POC1345 and POC1346; respectively

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1430

Plasmid

#221577

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1426, POC1427, POC1428 and POC1429; respectively

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTatAkBxCs

Plasmid

#178544

Purpose

pTatABC with KpnI, XbaI, and KpnI restriction sites after TatA, TatB, and TatC, respectively.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatB, TatC

Expression

Bacterial

Promoter

araC

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 SCAF8_isoD

Plasmid

#122467

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF8 isoform D (SCAF8 Human)

Use

Gateway Cloning

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMV0021 [15ARE23bp-GFP]

Plasmid

#246420

Purpose

AHR GFP reporter plasmid in a lentiviral backbone

Depositor

Marco Jost

Article

Verma et al bioRxiv [Preprint]. 2025 Sep 15:2025.09.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

AHR responsive minimal promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pEVOL-pAzF

Plasmid

#31186

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

Depositor

Peter Schultz

Article

Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

Insert

M.j. p-azidohenylalanine RS (2 copies +tRNA)

Expression

Bacterial

Mutation

Y32T, E107N, D158P, I159L, L162Q, D286R

Available Since

Dec. 9, 2011

Availability

Academic Institutions and Nonprofits only

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8xGTIIC-luciferase

Plasmid

#34615

Purpose

YAP/TAZ-responsive synthetic promoter driving luciferase expression

Depositor

Stefano Piccolo

Article

Dupont et al Nature. 2011 Jun 8;474(7350):179-83. doi

Insert

synthetic TEAD luciferase reporter

Use

Luciferase

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-Mango II x 24

Plasmid

#127587

Purpose

Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

Depositor

David Rueda

Article

Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

Insert

mCherry-Mango II x24

Tags

mCherry

Expression

Mammalian

Promoter

pTRE Tet ON

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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LXRE_Luc

Plasmid

#177622

Purpose

synthetic 3X LXRE promoter linked to luciferase in the pTAL vector

Depositor

Thomas Burris

Article

Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

Insert

3X LXRE

Use

Luciferase

Expression

Mammalian

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet

Plasmid

#138086

Purpose

Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles

Depositor

Eva Frickel

Article

Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV promoter with heptamerized tet‐operat…

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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LV-TRE-VP64 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60625

Purpose

Expresses VP64 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP64 mouse MyoD

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCMVMagFRET-1

Plasmid

#50742

Purpose

Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-1

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pTR47m4

Plasmid

#104068

Purpose

Luciferase Reporter for Formaldehyde Sensing

Depositor

David Liu

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

xluxAB
frmR
frm Promoter m4

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

23r and 25th nucleotides in sequence mutated from…

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLys-M1

Plasmid

#109382

Purpose

Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Expression

Bacterial

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(synapto).cpsfGFP.miRFP670nano3

Plasmid

#214926

Purpose

Expresses presynaptic non-responsive control

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(synapto).cpsfGFP.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE10

Plasmid

#90344

Purpose

IRF3 - Viral response gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IRF3

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).cpSFGFP.tdTomato

Plasmid

#248076

Purpose

cpSFGFP control with non-responsive mIRFP670nano3

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.mIRFP670nano3

Use

AAV

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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HRE-oUnaG

Plasmid

#201712

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pUC-hrDNA-12.0

Plasmid

#206320

Purpose

A plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)

Depositor

Brian McStay

Article

van Sluis et al Methods Mol Biol. 2016;1455:3-14. doi: 1

Insert

Intergenic spacer of human rDNA repeat

Use

Cloning

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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RARe-RFP-PGK-H2BGFP

Plasmid

#241863

Purpose

retinoic acid response element (RARe)-driven fluorescent reporter

Depositor

Elaine Fuchs

Article

Tierney et al Science. 2024 Mar 8;383(6687):eadi7342.

Insert

RARe-RFP

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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