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AAV-F in pAR-9

Plasmid

#166921

Purpose

AAV rep/cap plasmid. Capsid is mouse brain tropic AAV-F

Depositor

Casey Maguire

Article

Hanlon et al Mol Ther Methods Clin Dev. 2019 Oct 23;1

Insert

REP/CAP

Use

AAV

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCR1162

Plasmid

#111098

Purpose

Expresses FANCA_-_89883048.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCA gRNA (FANCA Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC-Taq

Plasmid

#102793

Purpose

Expresses Taq DNA polymerase under WT T7 promoter

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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5n1 (560)

Plasmid

#70730

Purpose

TetR/PhlF/SrpR/LambdaCI/LacI BCD7 5-node v41-2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Inserts

srpR-ssrA
lambdaCI-ssrA
phlF-ssrA
lacI-ssrA
tetR-ssrA

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-UBF

Plasmid

#17656

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

UBF (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only

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Raptor_2 shRNA

Plasmid

#1858

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

Raptor (RPTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pJOE7706.1

Plasmid

#135075

Purpose

A shuttle vector for heterologous HA production with Corynebacterium glutamicum

Depositor

Josef Altenbuchner

Article

Hoffmann et al J Appl Microbiol. 2014 Sep;117(3):663-78

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMC8b-XI3up

Plasmid

#176170

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-3

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XI3dw

Plasmid

#176171

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-3

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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p3662 HPV-16 E2

Plasmid

#22457

Depositor

Peter Howley

Article

Sakai et al J Virol. 1996 Mar . 70(3):1602-11.

Insert

E2 WT

Expression

Mammalian

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 TDP-43 NLS1 YFP

Plasmid

#84912

Purpose

Mammalian expresion of TDP-43 NLS1 YFP

Depositor

Aaron Gitler

Article

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Mutation

K82A, R83A, K84A

Promoter

CMV

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pATT-Dest

Plasmid

#79770

Purpose

Staging plasmid for use in DIP transposition

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Type

Empty backbone

Use

Synthetic Biology; Staging plasmid for transposit…

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM167

Plasmid

#84092

Purpose

target miRNA167 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM167

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDDGFP_LEU2D

Plasmid

#58352

Purpose

Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

Depositor

Simon Newstead

Article

Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

Type

Empty backbone

Tags

GFP-His

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pACRISPR-KM

Plasmid

#203587

Purpose

A sgRNA expression plasmid for genome editing in Burkholderia

Depositor

Leonilde Moreira

Article

Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

Plasmid

#210525

Purpose

AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mtagBFP-KASH
U6-gRNA

Use

AAV

Expression

Mammalian

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v6 (fl)

Plasmid

#137816

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v6 (fl) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

R296K in the V6 region [Please see depositor comm…

Promoter

PGK

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCR1161

Plasmid

#111097

Purpose

Expresses FANCA_-_89883045.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCA gRNA (FANCA Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Pet28a_Human_H3.3

Plasmid

#42632

Depositor

Joe Landry

Insert

Human H3.3 (H3-3A Human)

Tags

Untagged

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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3' MBD3 TALEN

Plasmid

#52359

Purpose

TALEN vector for targeting human MBD3 locus (3')

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

NN NN HD NN NG NN NI HD HD HD HD NI NN NI HD HD NG

Use

TALEN

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only