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NAX_CAGGS_CAS9_m4_VPR_mcherry

Plasmid

#167886

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-DIO-mRuby2

Plasmid

#99117

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

DIO-mRuby2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5myc Rac1 wt

Plasmid

#37030

Depositor

Lawrence Kirschner

Article

Manchanda et al Oncogene. 2013 Jul 25;32(30):3491-9. doi

Insert

Rac1 (RAC1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pKV015

Plasmid

#64766

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA-GFP to a T7-RFP tag, invariable V5 tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGWB435

Plasmid

#68700

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Drp1 V2 S579D

Plasmid

#46343

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Expression

Mammalian

Mutation

S579D

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pDUP1

Plasmid

#223516

Purpose

inactive cogfp under control of Ptet, cogfp under control of Ptac

Depositor

Yolanda Schaerli

Article

Mihajlovic et al eLife. 2025 Apr 2;13:RP97216. doi: 10.75

Inserts

CoGFP
CoGFP inactivated

Tags

His tag

Expression

Bacterial

Mutation

anti-dimerization mutations S129G, 22 C154S, D156…

Promoter

Ptac, Ptac and Ptet, and Ptet

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-11

Plasmid

#172762

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-11

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-26

Plasmid

#172751

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-26

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Drp1 V2 S579A

Plasmid

#46342

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Expression

Mammalian

Mutation

S579A

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
TRE-UBC-mCD59-T2A-GFP

Plasmid

#205451

Purpose

lentiviral plasmid for expression of mouse CD59

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD59 (Cd59a Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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polyPRM5

Plasmid

#175232

Purpose

Bacterial expression and purification, mixed with polySH3 to form condensates

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

ABL1 (ABL1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMpGWB331

Plasmid

#68659

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Emerald Luc fused with PEST sequence [ELuc(PEST)]

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pFUGW-ZipACR-EYFP

Plasmid

#88844

Purpose

Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

ZipACR-NotI-EYFP

Use

Lentiviral

Tags

EYFP

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-GW182delta1

Plasmid

#11592

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

GW182 (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contains only aa1-498; SEE DEPOSITOR NOTES BELOW

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pJL1-MBP-4xDQNAT

Plasmid

#128390

Purpose

In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Maltose binding protein

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Mutation

A302T- please see depositor comment

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMpGWB316

Plasmid

#68644

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged tdTomato (tdTo…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro

Plasmid

#31480

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (ani target)

Use

Lentiviral

Mutation

mCherry has M9S, M16L to reduce background mCherr…

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-yibDp-Erv1p

Plasmid

#202483

Purpose

Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-1

Plasmid

#172767

Purpose

Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-1

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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