We narrowed to 9,980 results for: Uty

1. pLenti CMV Hygro dn-cHSF1

   Plasmid

   #134732

   Purpose

   Lentiviral expression vector for constitutively active dominant-negative HSF1

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

   Insert

   dn-cHSF1 (HSF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 186-202, 379−529

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti CMV/TO Zeo dn-cHSF1

   Plasmid

   #134734

   Purpose

   Lentiviral expression vector for inducible expression of a constitutively active dominant-negative HSF1

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

   Insert

   dn-cHSF1 (HSF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 186-201, 380−529

   Promoter

   CMV/TO

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDest40 DHFR.dn-cHSF1

   Plasmid

   #134735

   Purpose

   Mammalian expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

   Insert

   DHFR.dn-cHSF1 (HSF1 Human)
   

   Tags

   DHFR

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 186-202, 379−529

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti PGK Puro GFP-GABARAPL1 G116

   Plasmid

   #123243

   Purpose

   Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deleted amino acid 117. Stop codon after G116

   Promoter

   PGK

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

   Plasmid

   #68345

   Purpose

   A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

   Inserts

   puromycin
   hCas9
   

   Use

   CRISPR; Flp/frt

   Tags

   linked to red flouresent protein gene mKateII and…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLP-PIGA3 (pLP305)

   Plasmid

   #196992

   Purpose

   Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.

   Depositor

   Matthew Maurano

   Article

   Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti PGK Puro GFP-GABARAPL1 G116A

   Plasmid

   #123245

   Purpose

   Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Glycine 116 to Alanine

   Promoter

   PGK

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

   Plasmid

   #62580

   Purpose

   Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Mutation

   miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

   Plasmid

   #62581

   Purpose

   Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Mutation

   miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pBFC0984

    Plasmid

    #231992

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with 2xBsaI spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119 and pTet

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK MEK5-DD-mRFP1 neo

    Plasmid

    #47548

    Purpose

    Lentiviral expression vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

    Insert

    MEK5-DD-mRFP1 (MAP2K5 Human)
    

    Use

    Lentiviral

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    S311D, T315D

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

12. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

    Plasmid

    #219565

    Purpose

    3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

    Depositor

    Andrea Ventura

    Article

    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

    Insert

    HygroR_2A_H2B-GFP-N_loxP_mScarlet
    

    Use

    Unspecified

    Promoter

    EF-1a

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE-MCS(extended)-Puro (empty vector)

    Plasmid

    #172603

    Purpose

    Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    The multiple cloning site (MCS) has been edited/e…

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid

    #99278

    Purpose

    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

    Depositor

    Jordan Miller

    Article

    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

    Inserts

    H2B-mVenus-IRES-mCherry
    Puro
    

    Use

    Lentiviral

    Tags

    H2B, IRES, PGK, mCherry, and mVenus

    Promoter

    EF1 alpha-human and PGK-mouse

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

    Plasmid

    #218973

    Purpose

    Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
    

    Expression

    Mammalian

    Mutation

    mutant SRSF1 binding motif (AGTCTCCT)

    Promoter

    RSV LTR

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZ M2rtTA_CAGG TetON-Sox10

    Plasmid

    #115240

    Purpose

    RMCE donor vector for inducible expression of SOX10 constitutive expression of m2rtTA (generated from plasmid #112668)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Insert

    SOX10 (SOX10 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pF CAG luc IRES neo

    Plasmid

    #98294

    Purpose

    For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Inserts

    Firefly luciferase
    IRES
    Neomycin resistance gene (NeoR)
    

    Use

    Lentiviral and Luciferase

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBFC0993

    Plasmid

    #231993

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with negative control (RFP-targeting) spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119 and pTet

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB-6HIS-TEV-mEGFP,IRES-NGFR

    Plasmid

    #164521

    Purpose

    All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR

    Plasmid

    #157744

    Purpose

    knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    chlorotoxin containing chimeric antigen receptor targeting glioblastoma
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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