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pGex-6p2_FANCM_K819R

Plasmid

#221380

Purpose

Expresses FANCM_K819R in bacterial cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Fanconi anemia group M protein (FANCM Human)

Expression

Bacterial

Mutation

Changed lysine 819 to arginine

Promoter

tac

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEYFP_C1_ATRX

Plasmid

#221383

Purpose

Expresses ATRX in mammalian cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Transcriptional regulator ATRX (ATRX Human)

Expression

Mammalian

Mutation

K536N (Please see depositor comments)

Promoter

CMV

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSD068F

Plasmid

#218137

Purpose

Lentiviral vector expressing evolved degron SD36 fused to eGFP with mCherry control.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD36-eGFP-IRES2-mCherry

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD113A

Plasmid

#218133

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved degron SD56

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD56

Tags

rpoZ

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJM154

Plasmid

#218121

Purpose

Hybrid circuit displaying FKBP12 bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

FKBP12
luxAB

Use

Luciferase

Tags

434cI_RR69

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-Myc-Caspase-11_humanized-mutant

Plasmid

#214316

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Use

Retroviral

Tags

Myc

Mutation

KLS288YKT_KASIHS348TPRAKA

Promoter

MESV

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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2x3APWWP-IFP2.0C

Plasmid

#215752

Purpose

Expresses double domain of DNMT3A PWWP domain fused to C-terminal part of IFP2.0 for H3K36me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2x3APWWP-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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plx208-CMV-SspB-EGFP-VP16

Plasmid

#213532

Purpose

Expresses one of the split transcription factor component in mammalian cells

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-Myc-Caspase-4_R269D

Plasmid

#214310

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Mutation

R269D

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-Myc-Caspase-4_K356D

Plasmid

#214309

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Mutation

K356D

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pL0_EF-Fld

Plasmid

#202181

Purpose

Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Fld terminator

Use

Synthetic Biology

Mutation

None

Available Since

Feb. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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M_NES 54kDa

Plasmid

#201375

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

MAPK_NES-EGFP-4PrA

Expression

Mammalian

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Vd-ARATR-GFP

Plasmid

#208903

Purpose

encodes the arachnotocin receptor (oxytocin/vasopressin-like) from the mite Varroa destructor, tagged with GFP

Depositor

Christian Gruber

Article

Duerrauer et al Sci Rep. 2019 Dec 17;9(1):19295. doi: 10

Insert

ARATR

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_EF-P49

Plasmid

#202176

Purpose

Level 0 plasmid containing a terminator sequence from gene 49202 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

P49 terminator

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 19, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_DE-Per_sig_pep

Plasmid

#202152

Purpose

Level 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Peroxisome signal peptide

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 19, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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FB398

Plasmid

#203634

Purpose

Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

FB367+FB395

Use

Synthetic Biology

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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FB399

Plasmid

#203635

Purpose

Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

FB367+FB396

Use

Synthetic Biology

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2A D197,199N

Plasmid

#195704

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 124-211 of mouse SYT9 with D1…

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2B D330, 332N

Plasmid

#195705

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 253-344 of mouse SYT9 with D3…

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pPI528

Plasmid

#207442

Purpose

6xHis-SS-TEV-ErkB-T176A-Y178A bacterial expression vector - catalytically inactive

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkB

Use

Unspecified

Mutation

T176A, Y178A

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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