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Plasmid#19067Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, NeoDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only
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pLenti CMVtight Hygro DEST (w769-1)
Plasmid#26433DepositorTypeEmpty backboneUseLentiviral; Tet-on advanced destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-AP-Smo-YFP-DEST
Plasmid#49099PurposeExpressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell linesDepositorInsertSmo (Smo Mouse)
UseTagsYFP and acceptor peptide (AP)ExpressionMammalianMutationPromoterT7Available sinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Puro DEST (670-1)
Plasmid#17293Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, PuroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVtight Neo DEST (w770-1)
Plasmid#26432DepositorTypeEmpty backboneUseLentiviral; Tet-on advanced destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP DEST (736-1)
Plasmid#19732Purpose3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expressionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAi; Gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-EEA-5guides-PGK-Puro
Plasmid#102898PurposeEBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-5guides-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Blast DEST (w524-1)
Plasmid#19065Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, BlastÂDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2-Flag
Plasmid#41005DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Hygro DEST (693-2)
Plasmid#17291Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, HygroDepositorTypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVtight Puro DEST (w768-1)
Plasmid#26430DepositorTypeEmpty backboneUseLentiviral; Tet-on advanced destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Zeo DEST (644-1)
Plasmid#17294Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, ZeoDepositorTypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mScarlet-I-stop
Plasmid#186393PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Dest-pA-pgk-bsd
Plasmid#74918PurposeGateway destination piggyBac vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV GFP-LC3B G120A
Plasmid#123115PurposeExpresses EGFP-LC3B G120A in mammalian cells. Negative control fluorescent reporter, unable to localize to autophagosomes.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsEGFPExpressionMammalianMutationGlycine 120 to AlaninePromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Hygro DEST H2B-mCerulean3
Plasmid#90234PurposeLentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)DepositorInsertH2B
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA
Plasmid#105648PurposeExpression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeksDepositorInsertTol2-Isl2b:GFP:polyA (isl2b Zebrafish)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Neo DEST (685-3)
Plasmid#17292Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, NeoDepositorTypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEF5-FRT-V5-DEST-GFP-Kif26b
Plasmid#102862PurposeExpresses GFP-Kif26b in mammalian cellsDepositorInsertMouse Kif26b (Kif26b Mouse)
UseTagsGFPExpressionMammalianMutationPromoterEF1AAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-Halotag-stop
Plasmid#186387PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Halo tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:icre-p2a-tomato
Plasmid#135217PurposeCre coexpressed with tdTomato in macrophagesDepositorInsertmfap4 promoter with iCre and Tomato
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1
Plasmid#52504Purposemammalian expression vector of BRCA1 wildtype siRNA resistantDepositorInsertBRCA1 (BRCA1 Human)
UseTagsFlagExpressionMammalianMutationsiRNA resistant sequence: AGTATAATCPromoterCMVAvailable sinceFeb. 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.2/DEST/hCx43-E2HA-stop
Plasmid#40909DepositorInsertConnexin 43 internal HA tag (GJA1 Human)
UseTagsExpressionMammalianMutationHA tag inserted in second extracellular loopPromoterCMVAvailable sinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET empty polycistronic destination vector (2D)
Plasmid#29774DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVtight Blast DEST (w762-1)
Plasmid#26434DepositorTypeEmpty backboneUseLentiviral; Tet-on advanced destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-N-Tandem-mCherry-EGFP
Plasmid#123216PurposeN-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Golden-Gate-Esp3I-Destination
Plasmid#51278PurposeCAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3IDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-LC3B-GFP
Plasmid#123107PurposeExpresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3BDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTags3xFLAG and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I tension sensor (F40-based)
Plasmid#119186PurposeThe F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.DepositorInserthuman Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Tol2-PA2-CMV-AB-mCh
Plasmid#160435PurposeExpresses human Amyloid Beta-mCherry in ZebrafishDepositorInsertHuman Amyloid Beta peptide (1-42) (APP Human, Zebrafish)
UseZebrafish expressionTagsmCherryExpressionMutationPromoterCMVAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALS099 (pGEX-2TP-cTevHis-DEST)
Plasmid#139787PurposeGateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable sinceApril 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS
Plasmid#113436PurposeExpresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cellsDepositorInserttype II-C anti-CRISPR
UseCRISPRTagsFLAG/NLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET empty polycistronic destination vector (2E)
Plasmid#29775DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMMACE DEST CMV H2B iRFP
Plasmid#206253PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.DepositorInsertH2B iRFP713
UseRecombinant baculovirus production, multimate/gat…TagsiRFP713ExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-GABARAP-GFP
Plasmid#123111PurposeExpresses 3xFLAG-GABARAP-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseTags3xFLAG and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-LC3A-GFP
Plasmid#123106PurposeExpresses 3xFLAG-LC3A-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3ADepositorInsertMicrotubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
UseTags3xFLAG and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
IY601: pMVP/Lenti/Blast-DEST
Plasmid#121849PurposepMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUseLentiviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-GABARAPL1-GFP
Plasmid#123112PurposeExpresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseTags3xFLAG and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I no force control (F40-based)
Plasmid#119187PurposeThe no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between mTFP1 and mEYFP that are tension-independent.DepositorInserthuman Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP] (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationtruncation after aa1945PromoterTCEAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTR AA Clover
Plasmid#90238PurposeLentiviral vector to express JNK KTR AA (mutant) mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (AA mutant)
UseLentiviralTagsmCloverExpressionMammalianMutationPromoterPGKAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only