We narrowed to 159,093 results for: Des
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Plasmid#226552PurposeBacterial expression of N-terminally 6His tagged A1-LCD X10DepositorInsertA1-LCD swap X10
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest-pol2-Smarcd1-Myc
Plasmid#227716PurposeExpresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.DepositorAvailable SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ScChd1[R126A/R130A]
Plasmid#221197PurposeScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutationsDepositorInsertScChd1 (aa118-1274)
Tags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationR126A, R130APromoterT7Available SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
N-SF-TAP-DEST-TLK2-D592V
Plasmid#218835PurposeExpresses N-terminal Strep-FLAG-tagged human TLK2 mutant D592V (kinase dead).DepositorInsertTLK2 Q592V (kinase dead) (TLK2 Human)
TagsFLAG-STREPExpressionMammalianMutationQ592VPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:mApple-he1.1:CFP
Plasmid#184812PurposeUsed to generate the Tg(QUAS:mApple; he1.1:CFP)c788 transgenic lineDepositorInsertQUAS:mApple-pA; he1.1:CFP
UseZebrafish tol2 transgenesisAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB-SNAP
Plasmid#166146PurposeGateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.DepositorTypeEmpty backboneTagsHis10 and SNAPExpressionBacterialAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-ccdB
Plasmid#166147PurposeGateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.DepositorTypeEmpty backboneTagsHis10-Smt3ExpressionBacterialAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-DDX5(kf)-SNAP
Plasmid#166152PurposeExpression of N. furzeri DDX5 in E. coliDepositorInsertDDX5
TagsHis10 and SNAPExpressionBacterialPromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher (Min)
Plasmid#216713PurposeExpresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014DepositorInsertCys-SpyCatcher (Min)
Tags6xHisExpressionBacterialMutationSee Li...Howarth, J. Mol. Biol., 2014Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDESTpK7WG2-RPS5Ap-NLS-OleGFP
Plasmid#193370PurposenTALEN or nTALECD vector assembly, Destination vector for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.DepositorTypeEmpty backboneExpressionPlantAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA ins:Apollo-NADP+ mVenus
Plasmid#210518PurposeExpresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoterDepositorInsertApollo-NADP+
UseZebrafish expression, tol2 kit gateway-compatibleTagsmVenusPromoterZebrafish insulin promoterAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-Dest-loxP-sfGFP
Plasmid#202715PurposeEmpty expression vector containing Cre recombinase reporter.DepositorInsertPw7-loxP-term-loxP-sfGFP
UseCre/LoxExpressionBacterialPromoterconstitutiveAvailable SinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMACE DEST CMV PE2
Plasmid#206276PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.DepositorInsertPE2
UseCRISPR; Recombinant baculovirus production, multi…ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-Blast-Dest-LifeAct-mRuby
Plasmid#194907PurposeTo generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.DepositorInsertLifeAct
UseMouse TargetingTagsmRubyExpressionMammalianAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Myc-FBW7]K404R
Plasmid#200715PurposeThe plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for overexpression and detection by western blotting.DepositorInsertFBW7 (FBXW7 Human)
TagsMycExpressionMammalianMutationFBW7 K404 is mutated to Alanine.PromoterCMVAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Myc-FBW7K404/412/R
Plasmid#200716PurposeThe plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 & 412 to Arg mutation. Used for mammalian overexpression and detection by western blotting.DepositorInsertFBW7 (FBXW7 Human)
TagsMycExpressionMammalianMutationFBW7 K404 and K412 mutated to Alanines.PromoterCMVAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
mKate2-DrCav1 in pT3TS-Dest
Plasmid#194293PurposeIn vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZWDepositorInsertcaveolin (cav1.S Frog)
UseIn vitro transcription of mrnaAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLRX-roGFP2 in pT3TS-Dest
Plasmid#194297PurposeIn vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRKDepositorInsertGLRX-roGFP2
UseIn vitro transcription of mrnaAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78)
Plasmid#194175PurposeExpresses lncRNA HIF1a-AS1 mutantDepositorInsertHIF1a-AS1 (HIF1A-AS1 Human)
ExpressionMammalianMutationWithin Exon1, nucleotides 26-78 were removedPromoterCMVAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXP1-DEST-Noa1-HIS
Plasmid#191972PurposeDestination vector containing HIS-tagged NOA1 coding sequence.DepositorAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
mKate2-DrCavin1a in pT3TS-Dest
Plasmid#194294PurposeIn vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAIDepositorInsertcavin1a (cavin1a Zebrafish)
UseIn vitro transcription of mrnaAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DEST47-Noa1-GFP
Plasmid#191968PurposeGateway Destination Vector, containing NOA1-GFP chimera coding sequence, for Gateway Cloning TechnologyDepositorInsertNoa1-GFP
UseSynthetic BiologyAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Dest-MBP-Nsp1
Plasmid#191483PurposeMammalian expression of MBP-tagged SARS-CoV-2 Nsp1DepositorInsertMBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionMammalianAvailable SinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::HA-RfA
Plasmid#186404PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pDEST-pSP172BSSPE-pFOGc::RfA
Plasmid#186397PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA
Plasmid#186396PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-Venus-RfA
Plasmid#186371PurposeDestination vector for the expression of a fluorescent fusion of N-ter Venus and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST tol2 UbB polyA
Plasmid#188701PurposeGateway destination vector containing the zebrafish ubiquitin B polyadenylation sequenceDepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianAvailable SinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-HA
Plasmid#186405PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDEST-pSP172BSSPE-pFOGc::RfA-HA
Plasmid#186406PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdestMB14_hsp-16.2p-grh-1-gfp
Plasmid#177762PurposeOverexpression of C. elegans grh-1DepositorInsertgrh-1 (grh-1 Nematode)
ExpressionWormAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos2-dVHH[GFP] in pCSDEST
Plasmid#162870PurposeSuper-resolution of GFP in cultureDepositorInsertmEos2-dVHH[EGFP]
TagsmEos2ExpressionMammalianAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-313 (attR4-attR3)
Plasmid#161898PurposeMultisite Gateway (attR4-attR3) vector for mammalian protein expressionDepositorTypeEmpty backboneExpressionMammalianAvailable SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3566 (pDEST15_Spt6 1223-1451)
Plasmid#91814PurposeBacterial expression of residues 1223-1451 from S. cerevisiae Spt6DepositorInsertSPT6 (SPT6 Budding Yeast)
TagsGSTExpressionBacterialMutationdeleted residues 1-1222PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-894 (attR4-attR2)
Plasmid#161893PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins in E. coliDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-639 (attR4-attR2)
Plasmid#161880PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneExpressionInsectAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-624 (attR4-attR2)
Plasmid#161879PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneExpressionInsectAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-DEST-R5R4-pHygTK
Plasmid#141059PurposeMutisite Gateway mediated vector constructionDepositorInsertHygTK
ExpressionMammalianAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-DEST-R5R4-pNeo
Plasmid#141058PurposeMutisite Gateway mediated vector constructionDepositorInsertNeomycin resistance gene
ExpressionMammalianAvailable SinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only