We narrowed to 16,194 results for: She

1. pRK5-EGFP-Tau P301L

   Plasmid

   #46908

   Purpose

   expresses EGFP tagged Tau P301L in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau P301L (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   P301L

   Promoter

   CMV

   Available since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3 1 kb prom. + Enhancer CD274

   Plasmid

   #107005

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   N.B. There are two published SNPs in this region …

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-6p1-GST-ArcFL

   Plasmid

   #119877

   Purpose

   Bacterial Expression construct for protein production and purification

   Depositor

   Jason Shepherd

   Article

   Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

   Insert

   Activity Regulated Cytoskeletal Protein (Arc Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC119-gRNA

   Plasmid

   #52255

   Purpose

   Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

   Depositor

   Jen Sheen

   Article

   Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

   Insert

   guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   Arabidopsis U6 polymerase III promoter

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. R702-X38-635: His6-Hs.NF1opt (1-2839) isoform 1

   Plasmid

   #159576

   Purpose

   Baculovirus protein expression of His6-Hs.NF1opt (1-2839) isoform 1

   Depositor

   Dominic Esposito

   Article

   Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

   Insert

   His6-Hs.NF1opt (1-2839) isoform 1 (NF1 Human)
   

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLVX-TetOne-Puro-PSMD4

   Plasmid

   #202666

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of PSMD4 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   proteasome 26S subunit ubiquitin receptor, non-ATPase 4 (PSMD4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TP53-KO_gRNA_1

   Plasmid

   #195130

   Purpose

   TP53-targeting gRNA in the LRG2.1 backbone

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   TP53 KO gRNA 1 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE_HRASV12_Hyg

   Plasmid

   #195143

   Purpose

   HRas G12V overexpression vector with hygroymycin resistance

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   hRAS (HRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEAT8-137 M1V

   Plasmid

   #173807

   Purpose

   Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells

   Depositor

   Anne Gershenson

   Article

   Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi

   Insert

   SERPiNA1 (SERPINA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E1M Otherwise this plasmid encodes the most commo…

   Promoter

   T7

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5-Flag-Ago2

    Plasmid

    #72207

    Purpose

    Mammalian expression of full-length human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE-ProDOL

    Plasmid

    #206866

    Purpose

    Expression of ProDOL probe - LynAnchor-eGFP-SNAP-tag-HaloTag

    Depositor

    Dirk-Peter Herten

    Article

    Tashev et al Nat Methods. 2024 Sep;21(9):1708-1715. d

    Insert

    ProDOL
    

    Use

    Retroviral

    Tags

    HaloTag, His-Tag, SNAP-tag, and eGFP

    Expression

    Mammalian

    Mutation

    Contains Lyn amino acids 1-16

    Promoter

    Available since

    Aug. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2

    Plasmid

    #159575

    Purpose

    Baculovirus protein expression of His6-tev-Hs.NF1opt (1-2818) isoform 2

    Depositor

    Dominic Esposito

    Article

    Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

    Insert

    His6-tev-Hs.NF1opt (1-2818) isoform 2 (NF1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28(+)-NudC

    Plasmid

    #175022

    Purpose

    Purification of NudC enzyme

    Depositor

    Jeppe Vinther

    Article

    Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

    Insert

    nudC
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-TRE-HTR6-SNAPf-TRIP

    Plasmid

    #189617

    Purpose

    Expresses cilia-targeted RhoA inhibitory peptide, tet-inducible

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    HTR6-TRIP_alpha
    

    Use

    AAV

    Tags

    alphaTag in the N-term of HTR6, SNAP-tag in the C…

    Expression

    Mutation

    Promoter

    TRE

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-NSD3-short W284A

    Plasmid

    #72551

    Purpose

    expresses 3*FLAG tagged human NSD3-short with W284A mutation

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    W284A

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TP53-KO_gRNA_2

    Plasmid

    #195131

    Purpose

    TP53-targeting gRNA in the LRG2.1 backbone

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    TP53 KO gRNA 2 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HOXB6 (human) FLAG pMSCV

    Plasmid

    #8534

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pSUPER-retro-puro-Smad2

    Plasmid

    #89827

    Purpose

    Knockdown human Smad2 expression in human mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

    Insert

    SMAD2 shRNA (SMAD2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. integrin alpha9 / alpha5 pcDNA1 neo

    Plasmid

    #13588

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 / alpha 5 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV-NSD3-short del(1-100)

    Plasmid

    #72547

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 1-100 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    1-100 aa deletion

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. integrin alpha9 / alpha4 pcDNA1 neo

    Plasmid

    #13587

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 / alpha 4 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. integrin alpha9 / alpha2 pcDNA1 neo

    Plasmid

    #13586

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 / alpha 2 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET14-H2Kb-ST3

    Plasmid

    #223221

    Purpose

    Expression of mouse MHC H2Kb heavy chain fused to Spytag003 under T7 promoter for expression in bacteria

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Expression of soluble mouse MHC H2KB heavy chain (HLA-A Mouse)
    

    Use

    Tags

    Spytag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLVX-Hyg-CMV-BCL9

    Plasmid

    #202667

    Purpose

    BCL9 overexpression vector with hygromycin resistance

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    BCL9 transcription coactivator (BCL9 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Halo-H1DBD-H2

    Plasmid

    #200205

    Purpose

    Expression of HSF2 with HSF1 DBD

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    Halo-H1DBD-H2 (Hsf1, Hsf2 Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    HSF1 (1-399); HSF2 (366-1554)

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. H2DBD-H1-Halo

    Plasmid

    #200193

    Purpose

    Expression of HSF1 with HSF2 DBD

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    H2DBD-H1-Halo (Hsf1, Hsf2 Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    HSF2 (1-366); HSF1 (399-1576)

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL3-MTS-COX2-Q6-R18-G1333C-UGI-Puro

    Plasmid

    #198845

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    COX2-Q6-R18 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL3-MTS-ATP8-W9-R15-G1333N-UGI-Puro

    Plasmid

    #198841

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ATP8-W9-R15 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGL3-MTS-ATP8-W9-L17-G1333C-UGI-Puro

    Plasmid

    #198840

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ATP8-W9-L17 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro

    Plasmid

    #198844

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    COX2-Q6-L18 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Donor_N_FH_Piwi

    Plasmid

    #186651

    Purpose

    Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Phage-PGK-Flag-HA-FKBP-T(WT)

    Plasmid

    #181734

    Purpose

    for lentiviral expression of brachyury ORF in mammalian cells

    Depositor

    Charles Lin

    Article

    Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

    Insert

    T (brachyury)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    a silent mutation in the PAM site of an sgRNAt ta…

    Promoter

    Available since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pRK5-EGFP-Tau

    Plasmid

    #46904

    Purpose

    expresses EGFP tagged Tau in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This WT htau construct contains human four-repeat…

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHR-SIN-BX SpyCatcher003-(short)mCD80

    Plasmid

    #210567

    Purpose

    Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Spycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLVX-Bsd-CMV-MDM4

    Plasmid

    #202669

    Purpose

    MDM4 overexpression vector with blasticidin resistance

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 regulator of p53 (MDM4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CYLBL_Neo_CAGdCas9-KRAB

    Plasmid

    #220439

    Purpose

    CAG-dCas9-KRAB knock-in using TALEN at CLYBL locus

    Depositor

    Yin Shen

    Article

    Yang et al Nat Genet. 2023 Oct;55(10):1735-1744. do

    Insert

    dCas9
    

    Use

    CRISPR and TALEN

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLVX-TetOne-Puro-MDM4

    Plasmid

    #195140

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of MDM4 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 (MDM4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEX-6p1-GST-ArcΔCTD

    Plasmid

    #119878

    Purpose

    Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

    Depositor

    Jason Shepherd

    Article

    Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

    Insert

    Activity Regulated Cytoskeletal Protein (Arc Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Deletion of nt 832-1110

    Promoter

    tac

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. alpha actinin delta ABD-GFP

    Plasmid

    #66935

    Purpose

    Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

    Depositor

    Pere Roca-Cusachs, Michael Sheetz

    Article

    Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Alpha-actinin 1 (ACTN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    actin binding domain (amino acids 30–253) is dele…

    Promoter

    CMV

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

40. 61426-hU6-shmFmr1-EF1a-mCherry

    Plasmid

    #157859

    Purpose

    Expresses a shRNA targeting mouse Fmr1 gene

    Depositor

    Xinyu Zhao

    Article

    Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

    Insert

    Fmr1 (Fmr1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge