We narrowed to 11,881 results for: pcDNA

1. 3494 pcDNA3 Bax

   Plasmid

   #8779

   Depositor

   Stanley Korsmeyer

   Article

   Oltvai et al Cell 1993 Aug 27;74(4):609-19.

   Insert

   Bax (Bax Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3-EGFP-Cdc42-wt

   Plasmid

   #12975

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Cdc42 (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-endo-GsCT

   Plasmid

   #205516

   Purpose

   Endosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-GsCT (GNAS Synthetic, Human)
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-FLAG-FADD

   Plasmid

   #78802

   Purpose

   To overexpress FADD in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Nat Commun. 2012;3:978. doi: 10.1038/nco

   Insert

   FADD (FADD Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-Flag Rac2

   Plasmid

   #12192

   Depositor

   Gary Johnson

   Article

   Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

   Insert

   Rac2 (RAC2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Neurl1Rm-Flag pcDNA3.1

   Plasmid

   #17319

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Koutelou et al J Biol Chem. 2007 Dec 12. ():.

   Insert

   Neuralized-like (Neurl1a Mouse)
   

   Use

   RNAi

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

7. Rat NCX1.1 (pcDNA3.1+)

   Plasmid

   #75190

   Purpose

   Mammalian expression of SLC8A1

   Depositor

   Jonathan Lytton

   Article

   Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

   Insert

   SLC8A1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 FLAG-BACH2

   Plasmid

   #232275

   Purpose

   Expression of FLAG-tagged BACH2 (H. sapiens) in human cell lines

   Depositor

   Michele Pagano

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   BACH2 (BACH2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3-HA-human MYCN

   Plasmid

   #74163

   Purpose

   Mammalian expression of human MycN

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   MycN (MYCN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-PKA-SPARK

    Plasmid

    #106920

    Purpose

    Express PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    PKA-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-Flag-NSF

    Plasmid

    #74924

    Purpose

    Mammalian expression of human NSF full-length (744 aa)

    Depositor

    Elisa Greggio

    Article

    Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

    Insert

    NSF (NSF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-2xFLAG-SREBP-2

    Plasmid

    #26807

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    L7V, G76S, K395R and I482L

    Promoter

    CMV

    Available since

    July 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-VEGFR2-mCherry

    Plasmid

    #139606

    Purpose

    Expresses a VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3/RGS-UBC12

    Plasmid

    #20080

    Depositor

    Edward Yeh

    Article

    Gong et al J Biol Chem. 1999 Apr 23;274(17):12036-4

    Insert

    UBC12 (UBE2M Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-Myc-ASC

    Plasmid

    #73952

    Purpose

    Express c-Myc-tagged human ASC in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

    Insert

    PYD and CARD domain containing, PYCARD (PYCARD Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+)-PKI-GFP

    Plasmid

    #118482

    Purpose

    GFP-tagged Protein Kinase A inhibitor peptide.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    PKI-GFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA_RGG-GFP-RGG

    Plasmid

    #124930

    Purpose

    Expression of RGG-GFP-RGG

    Depositor

    Matthew Good, Benjamin Schuster, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA6-N-Myc-SCAP

    Plasmid

    #134285

    Purpose

    Expresses Myc-tagged SCAP in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SCAP (Scap, Scap Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-nuc-Nb37

    Plasmid

    #205515

    Purpose

    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb37
    

    Use

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-nuc-GsCT

    Plasmid

    #205517

    Purpose

    Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-GsCT (GNAS Synthetic, Human)
    

    Use

    Tags

    Histone H2A and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-dCry-V5His

    Plasmid

    #136241

    Purpose

    Protein expression of D. melanogaster (Dm) cytochrome with V5 and His tags

    Depositor

    Aziz Sancar

    Article

    Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

    Insert

    DmCry
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-sACE2(WT)-Fc(IgG1)

    Plasmid

    #145163

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-FLAG-RIPK1

    Plasmid

    #78842

    Purpose

    To overexpress RIPK1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-CRISPR-Puro

    Plasmid

    #208646

    Purpose

    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA4-Flag-XPA

    Plasmid

    #22895

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    XPA (XPA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA5-Flag-Med26

    Plasmid

    #49250

    Purpose

    Mammalian expression of flag-tagged Med26

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med 26 (MED26 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3-smURFP-IRES-HO-1

    Plasmid

    #80345

    Purpose

    Expresses smURFP and Heme Oxygenase-1 in mammalian cells.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    smURFP
    HO-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5FRT/TO-UBXD3-Strep-HA

    Plasmid

    #113488

    Purpose

    Expression of human UBXD3 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD3 (UBXN10 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA3-SREBP-2-GFP

    Plasmid

    #204202

    Purpose

    Mammalian expression of SREBP-2

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-2 (SREBF2 Human)
    

    Use

    Tags

    T7x3 and eGFP

    Expression

    Mammalian

    Mutation

    multiple silent mutations

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1_ 2xFLAG-2xSTREP_NRF2

    Plasmid

    #159129

    Purpose

    expresses NRF2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1-FGFR2c-V5/HIS

    Plasmid

    #201107

    Purpose

    expression of human FGFR2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1+FLAG-KrascomG12D

    Plasmid

    #206844

    Purpose

    To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

    Depositor

    Christopher Counter

    Article

    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

    Insert

    Kras (Kras Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Codons altered to most optimum based on mouse cod…

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1(+)-FluoSTEP-ICUE

    Plasmid

    #181845

    Purpose

    Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-ICUE (RAPGEF3 Human)
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA5/FRT-HA-rM3D(Gs)

    Plasmid

    #45549

    Purpose

    Expresses rM3D (Gs) neuronal activator

    Depositor

    Bryan Roth

    Article

    Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

    Insert

    rM3D (Gs)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HA-Elongin B-pcDNA3.1(+)-Zeo

    Plasmid

    #20000

    Depositor

    William Kaelin

    Article

    Ohh et al Nat Cell Biol. 2000 Jul . 2(7):423-7.

    Insert

    transcription elongation factor B (ELOB Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA TfR1-miniR

    Plasmid

    #181986

    Purpose

    MiniR genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniE labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    TfR1-MiniR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-RIα-ICUE4

    Plasmid

    #181848

    Purpose

    ICUE4 cAMP sensor tethered to the PKA RIα subunit.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-ICUE4 (RAPGEF3, PRKAR1A Human)
    

    Use

    Tags

    ECFP, PKA RIα, and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA3_UnaG-Flag-Sec61B

    Plasmid

    #83413

    Purpose

    Expressed N-term tagged UnaG on Sec61B

    Depositor

    Hyun-Woo Rhee

    Article

    Park et al ACS Chem Biol. 2016 May 27.

    Insert

    UnaG-Sec61B (SEC61B Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-flag-YTHDF2-N

    Plasmid

    #52302

    Purpose

    Flag-tagged YTHDF2 N-terminal domain, made by mutating to E384(GAA) to stop codon(TAA)

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Changed E384 to stop codon

    Promoter

    T7

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pcDNA3-PDE4D2cat-ICUE4

    Plasmid

    #181847

    Purpose

    ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)
    

    Use

    Tags

    ECFP, PDE4D2(86-418), and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge