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We narrowed to 9,088 results for: ef1;

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Showing: 2351 - 2400 of 9088 results

  1. pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid
    #176460
    Purpose
    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Article
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Insert
    HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176460-plasmid-map-sequence-id-346305

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  2. pLenti_EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP_HygroR

    Plasmid
    #186744
    Purpose
    EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP expression construct cloned into a lentiviral backbone
    Depositor
    Richard Sherwood
    Article
    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.
    Insert
    EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP
    Use
    Lentiviral
    Tags
    P2A-BFP
    Expression
    Mammalian
    Available Since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    186744-plasmid-map-sequence-id-389262

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  3. pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

    Plasmid
    #187612
    Purpose
    Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii
    Inserts
    WWE domain (R163A) with C-terminal nLuc
    WWE domain with C-terminal cLuc
    Use
    Lentiviral
    Tags
    FLAG, HA, Split luciferase (cLuc), Split lucifera…
    Expression
    Mammalian
    Mutation
    Arg163Ala
    Promoter
    EF1A
    Available Since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187612-plasmid-map-sequence-id-372035

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  6. XZ377 (PB) EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export-IRESv0-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export

    Plasmid
    #233385
    Purpose
    PiggyBac donor plasmid with optimized VEGF-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as marker
    Depositor
    Xiaojing Gao
    Article
    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038
    Insert
    VEGFR1(ecto+TM)-ADAR2dd
    Tags
    FLAG, HA
    Expression
    Mammalian
    Mutation
    E488Q, T501A on ADAR2dd
    Promoter
    EF1a
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233385-plasmid-map-sequence-id-467482

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  7. XZ393 (PB) EF1a-IL2Rbsp-FLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export-IRESv0-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export

    Plasmid
    #233388
    Purpose
    PiggyBac donor plasmid with optimized IL-2-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as marker
    Depositor
    Xiaojing Gao
    Article
    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038
    Insert
    IL2Rb(ecto+TM)-ADAR2dd
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E488Q, T501A on ADAR2dd
    Promoter
    EF1a
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233388-plasmid-map-sequence-id-467483

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  8. pHIV-Luc-ZsGreen

    Plasmid
    #39196
    Depositor
    Bryan Welm
    Inserts
    Firefly Luciferase Luc2P
    ZsGreen
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-alpha and EF1-alpha
    Available Since
    Oct. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    39196-plasmid-map-sequence-id-335121

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  9. AV0.1-BWS-copGFP

    Plasmid
    #124967
    Purpose
    Express copGFP under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP
    Use
    AAV
    Promoter
    EF1-alpha
    Available Since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    124967-plasmid-map-sequence-id-243875

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  10. control (pGS701)

    Plasmid
    #160037
    Purpose
    pLV-A4-UCOE-EF1-3xHA-GFP-MCS-IRES-BLAS-2A-SNAP-WPRE-A2
    Depositor
    David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Promoter
    EF1
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    160037-plasmid-map-sequence-id-319591

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  11. pCDH-CMV-Nluc

    Plasmid
    #73038
    Purpose
    Express Nluc reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73038-plasmid-map-sequence-id-136036

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  12. ER-J-TAG (pGS730)

    Plasmid
    #160038
    Purpose
    pLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2
    Depositor
    David Sabatini
    Insert
    3xHA-sfGFP-JPH-TMD
    Use
    Lentiviral
    Promoter
    EF1
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    160038-plasmid-map-sequence-id-319597

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  13. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73037
    Purpose
    Express Nluc and copGFP dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    73037-plasmid-map-sequence-id-136876

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  14. pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73040
    Purpose
    Express Nluc and copGFP dual reporter in mammalian cells
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    PGK (phosphoglycerate kinase 1)
    Available Since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73040-plasmid-map-sequence-id-136039

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  15. pCDH-CB-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73039
    Purpose
    Express Nluc, copGFP dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    chicken beta-actin + CMV enhancer and chicken bet…
    Available Since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73039-plasmid-map-sequence-id-136043

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  18. pEF_dCas9-VP64

    Plasmid
    #68417
    Purpose
    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG and VP64
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68417-plasmid-map-sequence-id-120195

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  19. pJP72

    Plasmid
    #176479
    Purpose
    Expresses mScarlet under control of the Capsaspora EF1 promoter and GenR under control of the Capsaspora actin promoter
    Depositor
    Duojia Pan
    Article
    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1
    Inserts
    actin > GenR
    EF1 > mScarlet
    Use
    Capsaspora owczarzaki expression
    Available Since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176479-plasmid-map-sequence-id-346558

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  20. pJP102

    Plasmid
    #176480
    Purpose
    Expresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoter
    Depositor
    Duojia Pan
    Article
    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1
    Inserts
    actin > NatR
    EF1 > mScarlet
    Use
    Capsaspora owczarzaki expression
    Available Since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176480-plasmid-map-sequence-id-346562

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  21. pJP103

    Plasmid
    #176481
    Purpose
    Expresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter
    Depositor
    Duojia Pan
    Article
    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1
    Inserts
    actin > HygR
    EF1 > mScarlet
    Use
    Capsaspora owczarzaki expression
    Available Since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176481-plasmid-map-sequence-id-346559

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  26. pCAT005

    Plasmid
    #171632
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-B2M
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171632-plasmid-map-sequence-id-336487

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  28. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CAG and EF1-a
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

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  29. pJRH623

    Plasmid
    #171633
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-non-targeting
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171633-plasmid-map-sequence-id-336492

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  33. AV0-BWS-2.0

    Plasmid
    #124976
    Purpose
    Express gene of interest under EF1 promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    AAV
    Available Since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    124976-plasmid-map-sequence-id-243968

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  34. pLentiCRISPR_mCherry

    Plasmid
    #229078
    Purpose
    Small lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cells
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1s
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    229078-plasmid-map-sequence-id-456127

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  35. LZ10 PBREBAC-H2BHalo

    Plasmid
    #91564
    Purpose
    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.
    Depositor
    James Zhe Liu
    Article
    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1
    Insert
    histone H2B-HaloTag
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91564-plasmid-map-sequence-id-180643

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  36. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171625-plasmid-map-sequence-id-335505

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  38. HSC1-D4Z4-GiP

    Plasmid
    #58542
    Purpose
    Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Human EF1-alpha
    Available Since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58542-plasmid-map-sequence-id-90651

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  40. pLenti-EGFP-SnxA

    Plasmid
    #232460
    Purpose
    Lentiviral EGFP lipid sensor for PI(3,5)P2
    Depositor
    Ken-Ichi Takemaru
    Insert
    EGFP-SnxA
    Use
    Lentiviral
    Tags
    EGFP
    Promoter
    EF1 alpha
    Available Since
    March 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    232460-plasmid-map-sequence-id-464306

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  41. HSC1-HS4-D4Z4c-GiP

    Plasmid
    #58541
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Human EF1-alpha
    Available Since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58541-plasmid-map-sequence-id-90716

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  42. AV0-BWS-2.0-mC-G-CL3B

    Plasmid
    #124975
    Purpose
    evaluate autophagy
    Depositor
    Kazuhiro Oka
    Insert
    mCherry-GFP-LC3B
    Use
    AAV
    Promoter
    EF1-alpha
    Available Since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    124975-plasmid-map-sequence-id-243955

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  43. pWPI-myogenin

    Plasmid
    #79073
    Purpose
    Lentiviral plasmid that constitutively expresses human myogenin
    Depositor
    Jennifer Lei
    Insert
    human myogenin (MYOG Human)
    Use
    Lentiviral
    Promoter
    EF1 alpha
    Available Since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79073-plasmid-map-sequence-id-151242

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  44. ATG-2460

    Plasmid
    #108713
    Purpose
    EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)
    Depositor
    Keith Wood
    Article
    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10
    Insert
    CBR2opt-copGFP fusion
    Use
    Lentiviral
    Tags
    CBR2opt fused to copGFP
    Mutation
    Promega used directed evolution to create CBR (Cl…
    Promoter
    elongation factor-1
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108713-plasmid-map-sequence-id-224540

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  45. pLenti-EGFP-2xFYVE

    Plasmid
    #136996
    Purpose
    Lentiviral EGFP lipid sensor for PI(3)P
    Depositor
    Ken-Ichi Takemaru
    Insert
    EGFP-2xFYVE
    Use
    Lentiviral
    Tags
    EGFP
    Promoter
    EF1 alpha
    Available Since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    136996-plasmid-map-sequence-id-270659

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  46. pJG01

    Plasmid
    #213505
    Purpose
    expresses TdTomato in Capsaspora owczarzaki
    Depositor
    Joseph Gerdt
    Insert
    TdTomato
    Promoter
    EF1
    Available Since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    213505-plasmid-map-sequence-id-431905

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  47. pLV_Zika_NS3_Flag

    Plasmid
    #79635
    Purpose
    Third generation Self-inactivating lentivector expressing NS3 from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS3
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79635-plasmid-map-sequence-id-152697

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  48. pLV_Zika_NS5_Flag

    Plasmid
    #79639
    Purpose
    Third generation Self-inactivating lentivector expressing NS5 from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS5
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    79639-plasmid-map-sequence-id-148807

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  49. pLenti-EGFP-P4M-SidMx2

    Plasmid
    #136997
    Purpose
    Lentiviral EGFP lipid sensor for PI(4)P
    Depositor
    Ken-Ichi Takemaru
    Insert
    EGFP-P4M-SidMx2
    Use
    Lentiviral
    Tags
    EGFP
    Promoter
    EF1 alpha
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    136997-plasmid-map-sequence-id-291840

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  50. pLV_Zika_NS2B_Flag

    Plasmid
    #79637
    Purpose
    Third generation Self-inactivating lentivector expressing NS2B from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS2B
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79637-plasmid-map-sequence-id-151178

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Showing: 2351 - 2400 of 9088 results