We narrowed to 8,806 results for: ef1;

1. pLKO5.sgRNA.EFS.tRFP

   Plasmid

   #57823

   Purpose

   3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   tagRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PB_tre_dCas9_KRAB

   Plasmid

   #126030

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with KRAB
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLKO5.sgRNA.EFS.GFP

   Plasmid

   #57822

   Purpose

   3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PB_tre_dCas9_VP160

   Plasmid

   #126031

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with VP160
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast

   Plasmid

   #205555

   Purpose

   Express EPN24-MCP-IRES-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-MCP
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

   Plasmid

   #205550

   Purpose

   Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-MCP
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO5.sgRNA.EFS.tRFP657

   Plasmid

   #57824

   Purpose

   Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   tagRFP657
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF-XMAS-1xStop

   Plasmid

   #164411

   Purpose

   Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   T2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJAM1.21_PB-UGCG-PGK-Blast

   Plasmid

   #205566

   Purpose

   Express human UGCG

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   UGCG (UGCG Human)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAS_4xMma PylT sfGPF 150TAG

    Plasmid

    #154766

    Purpose

    plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in GFP reporter, U25C in PylT

    Promoter

    EF1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAS_4xMma PylT_FLAG-Mma PylRS AF

    Plasmid

    #140023

    Purpose

    Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y271A, Y349F

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAS_4xMx PylT_FLAG-Mx PylRS

    Plasmid

    #140011

    Purpose

    Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAS_4xMx PylT_GFP 150TAG

    Plasmid

    #140016

    Purpose

    Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG, K46E (Please see depositor comments below)

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV/Guide-mCherry

    Plasmid

    #170543

    Purpose

    Expresses mCherry and contains a cassette for U6 driven sgRNA transcription

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDH_SBP-EGFP-GPI

    Plasmid

    #65298

    Purpose

    synchronize trafficking of EGFP-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Use

    Lentiviral

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAS_4xMma PylT_FLAG-Mma PylRS

    Plasmid

    #140009

    Purpose

    Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDH-TfR-mApple

    Plasmid

    #179384

    Purpose

    Lentiviral vector for expression of Human Transferrin receptor-1 fused mApple

    Depositor

    Angus Johnston

    Article

    Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

    Insert

    Transferrin receptor 1 - mApple (TFRC Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFH2.217_PB-EPN24-PCP-IRES-eGFP-PGK-Blast

    Plasmid

    #205557

    Purpose

    Express EPN24-PCP-IRES-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-PCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HSC1-GiP

    Plasmid

    #58254

    Purpose

    This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1-alpha

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CRISPR-SP-Cas9 reporter

    Plasmid

    #62733

    Purpose

    Mammalian CRISPR-SP-Cas9 reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    CRISPR-SP-Cas9 target seq + tdTomato (out of frame)
    

    Use

    CRISPR and Lentiviral

    Tags

    CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-Alpha

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDH_Str-KDEL_neomycin

    Plasmid

    #65307

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    

    Use

    Lentiviral

    Tags

    KDEL motif and Signal Sequence

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJAM1.17_PB-STEAP3-PGK-Blast

    Plasmid

    #205562

    Purpose

    Express human STEAP3

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    STEAP3 (STEAP3 Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFH2.101_PB-EPN11-MCP-T2A-eGFP-PGK-Blast

    Plasmid

    #205546

    Purpose

    Express EPN11-MCP-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN11-MCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pWPT-/GCCACC-mEGFP-IRES-mCherry

    Plasmid

    #49235

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWPI/hPLK2WT/Neo

    Plasmid

    #35385

    Depositor

    Anurag Tandon

    Article

    Visanji et al J Biol Chem. 2011 Oct 14;286(41):35863-7

    Insert

    hPLK2 (PLK2 Human)
    

    Use

    Lentiviral

    Tags

    EMCV IRES-Neomycin cassette and V5 epitope and Po…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    April 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEF-ABEmax

    Plasmid

    #164415

    Purpose

    EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    ABEmax
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCEP4_WT_OCT4

    Plasmid

    #40629

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pJAM1.18_PB-SDC4-PGK-Blast

    Plasmid

    #205563

    Purpose

    Express human SDC4

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    SDC4 (SDC4 Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDH_SBP-EGFP-Ecadherin

    Plasmid

    #65290

    Purpose

    Ecadherin reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Lentiviral

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pWPI/hPLK2K111M/Neo

    Plasmid

    #35386

    Depositor

    Anurag Tandon

    Article

    Visanji et al J Biol Chem. 2011 Oct 14;286(41):35863-7

    Insert

    hPLK2 K111M (PLK2 Human)
    

    Use

    Lentiviral

    Tags

    EMCV IRES-Neomycin cassette and V5 epitope and Po…

    Expression

    Mammalian

    Mutation

    K111M

    Promoter

    EF1-alpha

    Available since

    April 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDH_SBP-mCherry-GPI

    Plasmid

    #65299

    Purpose

    synchronize trafficking of mCherry-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Use

    Lentiviral

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast

    Plasmid

    #205549

    Purpose

    Express EPN24-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCRISPRai

    Plasmid

    #189938

    Purpose

    Expresses CRISPRai cassette under EF1a promoter

    Depositor

    Yu-Chen Hu

    Article

    Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

    Insert

    CRISPRai cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer-rat EF1 alpha

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDH_SBP-mCherry-Ecadherin

    Plasmid

    #65291

    Purpose

    Ecadherin reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Lentiviral

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SEP-Nanoluc-cyOFP1

    Plasmid

    #141082

    Purpose

    prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEP-Nanoluc-cyOFP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of amino acids DISGG from positions 673 …

    Promoter

    EF1α

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SGL40C-H1.EFS.RFP657

    Plasmid

    #69148

    Purpose

    Advanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEF-XMAS-2xStop

    Plasmid

    #164412

    Purpose

    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCEP4_OCT4_T234A_S235A

    Plasmid

    #40630

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1_S234A_T235A (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S234A_T235A

    Promoter

    EF1-alpha

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

39. pCEP4_OCT4_T234E_S235E

    Plasmid

    #40631

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T234E_S235E

    Promoter

    EF1-alpha

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast

    Plasmid

    #205542

    Purpose

    Express EPN1-MCP-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN1-MCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pWPT-/CGA-mEGFP-IRES-mCherry

    Plasmid

    #49233

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pFH2.96_PB-EPN1-T2A-eGFP-PGK-Blast

    Plasmid

    #205541

    Purpose

    Express EPN1-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN1
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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43. pFH2.100_PB-EPN11-T2A-eGFP-PGK-Blast

    Plasmid

    #205545

    Purpose

    Express EPN11-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN11
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pAS_4xMx PylT C41CA_GFP 150TAG

    Plasmid

    #140017

    Purpose

    Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pScalps eSpCas9_Zsgreen gRNA scaffold

    Plasmid

    #188449

    Purpose

    Expression of eSpCas9 tagged with Zsgreen

    Depositor

    Ajit Chande

    Article

    Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. SGL40C.EFS.RFP657

    Plasmid

    #69147

    Purpose

    Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAS_4xhyb PylT sfGFP 150TAG

    Plasmid

    #154771

    Purpose

    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCDH-AtAFB2-mTagBFP2

    Plasmid

    #179889

    Purpose

    Expresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    AtAFB2 (AFB2 Synthetic, Mustard Weed)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pWPT-ACC/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49229

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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50. pPKm-145

    Plasmid

    #90505

    Purpose

    Empty plasmid, pSIN-EF1-alpha-IRES-puro

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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