We narrowed to 1,270 results for: MS2

1. pYPQ141-ZmUbi-RZ-Aa1.2.3

   Plasmid

   #136372

   Purpose

   Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 with MS2 at the third stem loop is used

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGMC00028 (aka pTS0057)

   Plasmid

   #200832

   Purpose

   AAVS1 guide RNA cloned into the lentiSAM puro backbone

   Depositor

   Raj Chari

   Article

   Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

   Insert

   AAVS1 guide RNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pA-CBh.TP-CNOT8-V5H6.MCh.Puro

   Plasmid

   #209947

   Purpose

   In mammalian cells expresses TP fused to a CNOT8 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

   Depositor

   Kevin Weeks

   Article

   Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

   Inserts

   CCR4-NOT transcription complex subunit 8 (CNOT8 Human)
   mCherry fluorescent protein fused to puromycin resistance marker
   

   Tags

   TP (MS2 coat protein) and V5 epitope with H6 tag

   Expression

   Mammalian

   Promoter

   CBh and CMV

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cas12f-GE ver4.1

   Plasmid

   #176544

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver4.1
   Cas12f-GE ver4.1
   

   Expression

   Mammalian

   Promoter

   U6 and chicken β-actin

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas12f-GE ver4.0

   Plasmid

   #176543

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver4.0
   Cas12f-GE ver4.0
   

   Expression

   Mammalian

   Promoter

   U6 and chicken β-actin

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas12f-GE ver3.0

   Plasmid

   #176508

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver3.0
   Cas12f-GE ver3.0
   

   Expression

   Mammalian

   Promoter

   U6 and chicken β-actin

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPLINTR GFP v1 backbone

   Plasmid

   #179777

   Purpose

   SPLINTR backbone vector

   Depositor

   Mark Dawson

   Article

   Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)

   Plasmid

   #160571

   Purpose

   Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   sgRNAscaffold 2.1 scRNA
   

   Use

   CRISPR

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pA-CBh.TP-CNOT7-V5H6.MCh.Puro

   Plasmid

   #209946

   Purpose

   In mammalian cells expresses TP fused to a CNOT7 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

   Depositor

   Kevin Weeks

   Article

   Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

   Inserts

   CCR4-NOT transcription complex subunit 7 (CNOT7 Human)
   mCherry fluorescent protein fused to puromycin resistance marker
   

   Tags

   TP (MS2 coat protein) and V5 epitope with H6 tag

   Expression

   Mammalian

   Promoter

   CBh and CMV

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pA-CBh.TP-DCP2-V5H6.MCh.Puro

    Plasmid

    #209948

    Purpose

    In mammalian cells expresses TP fused to a DCP2 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

    Depositor

    Kevin Weeks

    Article

    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

    Inserts

    Decapping protein 2 (DCP2 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    

    Tags

    TP (MS2 coat protein) and V5 epitope with H6 tag

    Expression

    Mammalian

    Promoter

    CBh and CMV

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pA-CBh.TP-(inactive)CNOT6-V5H6.MCh.Puro

    Plasmid

    #209943

    Purpose

    In mammalian cells expresses TP fused to an inactive CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

    Depositor

    Kevin Weeks

    Article

    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

    Inserts

    CCR4-NOT transcription complex subunit 6 (enzymatically inactive) (CNOT6 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    

    Tags

    TP (MS2 coat protein) and V5 epitope with H6 tag

    Expression

    Mammalian

    Promoter

    CBh and CMV

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pA-CBh.TP-CNOT6-V5H6.MCh.Puro

    Plasmid

    #209944

    Purpose

    In mammalian cells expresses TP fused to a CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

    Depositor

    Kevin Weeks

    Article

    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

    Inserts

    CCR4-NOT transcription complex subunit 6 (CNOT6 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    

    Tags

    TP (MS2 coat protein) and V5 epitope with H6 tag

    Expression

    Mammalian

    Promoter

    CBh and CMV

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pA-CBh.TP-CNOT6L-V5H6.MCh.Puro

    Plasmid

    #209945

    Purpose

    In mammalian cells expresses TP fused to a CNOT6L with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

    Depositor

    Kevin Weeks

    Article

    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

    Inserts

    CNOT6L (CNOT6L Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    

    Tags

    TP (MS2 coat protein) and V5 epitope with H6 tag

    Expression

    Mammalian

    Promoter

    CBh and CMV

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. (attB) tdMCP-tagged BRaf

    Plasmid

    #226165

    Purpose

    Used to integrate 3xFlag-tdMCP-BRaf without MS2-circRNA barcdes into landing pad cell lines containing a single promoter.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    3xFlag-tdMCP-BRaf (BRAF Human)
    

    Use

    Bxb1 attb, no promoter

    Tags

    3xFlag-tdMCP and IRES-mCherry-P2A-PuroR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUPD2 sgRNA sCRNA 2.0 (GB2461)

    Plasmid

    #160593

    Purpose

    Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNA sCRNA 2.0
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ269E2

    Plasmid

    #164721

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-zCas9(D10A)-UGI-T2A-MS2-UGI
    

    Use

    CRISPR; Gateway compatible a3a/y130f-cbe_v04 entr…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-iSAM

    Plasmid

    #211495

    Purpose

    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

    Depositor

    Lesley Forrester, Antonella Fidanza

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-mCherry Tag

    Expression

    Mammalian

    Promoter

    EF1a, NA, Splicing acceptor, and TREGV promoter

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVD1 M1_1-pTRNA-scf 2.1 (GB2603)

    Plasmid

    #160568

    Purpose

    tRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    M1_1-pTRNA-scf 2.1
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

    Plasmid

    #160621

    Purpose

    Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    U6-26:gRNA 4 (pNos): 2.1 aptamer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ269C2

    Plasmid

    #164716

    Purpose

    Gateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    hAID-zCas9(D10A)-UGI-T2A-MS2-UGI
    

    Use

    CRISPR; Gateway compatible haid-cbe_v04 entry clo…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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