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We narrowed to 7,450 results for: ef1a

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Showing: 941 - 960 of 7450 results

  3. pEf1a-hifiDn29-dCas9-P2A-GFP

    Plasmid
    #247158
    Purpose
    Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Article
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Insert
    hifiDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…
    Promoter
    Ef1a
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247158-plasmid-map-sequence-id-491688

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  4. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

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  5. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

    Plasmid
    #192664
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB-MeCP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192664-plasmid-map-sequence-id-379868

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  14. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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  15. pEf1a-goldDn29-dCas9-P2A-GFP

    Plasmid
    #247157
    Purpose
    Mammalian expression of a human codon optimized engineered goldDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Article
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Insert
    goldDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N
    Promoter
    Ef1a
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247157-plasmid-map-sequence-id-491683

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  17. LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode

    Plasmid
    #170228
    Purpose
    Barcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    Map2k7-MAPK9 fusion (MAPK9, Map2k7 Mouse, Human)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    Fusion
    Promoter
    EF1a
    Available Since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170228-plasmid-map-sequence-id-334409

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Showing: 941 - 960 of 7450 results