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Showing: 941 - 960 of 24604 results

  4. FUCas9GFP

    Plasmid
    #85555
    Purpose
    Expresses Cas9 with green fluorescent reporter
    Depositor
    Marco Herold
    Insert
    humanised S.pyogenes Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    hUBC
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85555-plasmid-map-sequence-id-169910

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  8. pEDA5_GFPmut3_Y66H

    Plasmid
    #80085
    Purpose
    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.
    Depositor
    Timothy Whitehead
    Article
    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do
    Insert
    GFPmut3_Y66H
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    changed Tyrosine 66 to Histidine
    Promoter
    proB
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    80085-plasmid-map-sequence-id-197645

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  11. LCV2_EGFP

    Plasmid
    #155098
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155098-plasmid-map-sequence-id-307983

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  15. pCAGGS_3E5E_eGFP

    Plasmid
    #103054
    Purpose
    Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter
    Depositor
    Elke Mühlberger
    Article
    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do
    Insert
    Ebola virus (ebolavirus Zaire, Mayinga) minigenome
    Use
    Tags
    3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    103054-plasmid-map-sequence-id-197128

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  20. sgRNA.SFFV.GFP

    Plasmid
    #169938
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and GFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.
    Depositor
    Benjamin Ebert
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    169938-plasmid-map-sequence-id-338362

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Showing: 941 - 960 of 24604 results